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UTA Microbiology Exam 3 Questions and
Correct Answers/ Latest Update /
Already Graded
4 Basic Steps of binary fission
Ans: 1. Growth of cell size
2. Replication of DNA
3. Division of cytoplasm
4. Septum formation & cell division
Z Ring Assembly
Ans: FtsZ assembles Z ring to form divisome
Generation Time
Ans: time it takes to double the population
Lag Phase
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Ans: Cells adjust to culture medium; no change in population
What factors determine the duration of the lag phase?
Ans: Genetic makeup, media composition, initial inoculum size
During which phase of the growth curve is it most appropriate for the
use of antibiotics?
Ans: Log phase
Following the death phase of the growth curve, ____ cells are
considered the surviving cells & are considered clinically important
due to their association with chronic infections, such as Tuberculosis.
Ans: Persisters
Log Phase
Ans: Binary fission occurs; cell replication > cell death
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Stationary Phase
Ans: Resources become depleted; cell respiration=cell death
Death Phase
Ans: Endospores can form; cell replication < cell death
Close System Cultures
Ans: Have finite resources
Open System Cultures
Ans: -Nutrients and air are replenished
-Dead cells and waste are removed
-Beneficial for industrial microbiology
Microscopic Cell Count
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Ans: Cells are counted under a microscope using a laser etched slide
In using the direct microscopic cell count method, you will be able
to distinguish between live vs dead cells.
Ans: False
Fluorescence Staining
Ans: Cells are counted under a microscope or flow cytometer
In fluorescent staining, the end product results in live cells staining
___________?
Ans: Green
In fluorescent staining, the end product results in dead cells staining
___________?
Ans: Red
UTA Microbiology Exam 3 Questions and
Correct Answers/ Latest Update /
Already Graded
4 Basic Steps of binary fission
Ans: 1. Growth of cell size
2. Replication of DNA
3. Division of cytoplasm
4. Septum formation & cell division
Z Ring Assembly
Ans: FtsZ assembles Z ring to form divisome
Generation Time
Ans: time it takes to double the population
Lag Phase
,2 | Page
Ans: Cells adjust to culture medium; no change in population
What factors determine the duration of the lag phase?
Ans: Genetic makeup, media composition, initial inoculum size
During which phase of the growth curve is it most appropriate for the
use of antibiotics?
Ans: Log phase
Following the death phase of the growth curve, ____ cells are
considered the surviving cells & are considered clinically important
due to their association with chronic infections, such as Tuberculosis.
Ans: Persisters
Log Phase
Ans: Binary fission occurs; cell replication > cell death
,3 | Page
Stationary Phase
Ans: Resources become depleted; cell respiration=cell death
Death Phase
Ans: Endospores can form; cell replication < cell death
Close System Cultures
Ans: Have finite resources
Open System Cultures
Ans: -Nutrients and air are replenished
-Dead cells and waste are removed
-Beneficial for industrial microbiology
Microscopic Cell Count
, 4 | Page
Ans: Cells are counted under a microscope using a laser etched slide
In using the direct microscopic cell count method, you will be able
to distinguish between live vs dead cells.
Ans: False
Fluorescence Staining
Ans: Cells are counted under a microscope or flow cytometer
In fluorescent staining, the end product results in live cells staining
___________?
Ans: Green
In fluorescent staining, the end product results in dead cells staining
___________?
Ans: Red
