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Chapter 13 Microbiology UTA Tholen
Questions and Correct Answers/ Latest
Update / Already Graded
Main goal to controlling microbial growth
Ans: reduce microbial load and reduce infection/contamination
Sterilization
Ans: removal and killing of ALL microbes from fomite (inanimate
object)/organism
Disinfection
Ans: inactivation/kill of microbes on fomites
Sanitization
Ans: decrease of microbial load (amt. of microbes)
Biological Safety Level
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Ans: Levels of cleanliness assigned to labs; CDC, NIH, WHO established 4
levels
BSL-1
Ans: microbes aren't known to cause disease in healthy host and pose
minimal risk to workers and the environment
-sink for handwashing, door to close off lab (ex. freshman bio labs)
-ex. non-pathogenic strains of Escherichia coli
BSL-2
Ans: microbes are typically indigenous and are associated with diseases
of varying severity; they pose moderate risk to workers and the
environment
-BSL-1 and PPE, self-closing doors, eyewash station, autoclave, or
sterilization method (ex. Micro labs)
-ex. Staphylococcus aureus
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BSL-3
Ans: microbes are indigenous/exotic and cause lethal diseases thru
respiratory transmission
-BSL-2 and respirator, bio safety cabinets, hands-free wash sink, two sets of
doors (ex. none at UTA)
-ex. Mycobacterium tuberculosis
BSL-4
Ans: - Microbes are dangerous & exotic, posing a
high risk of aerosol‐transmitted infections, which are frequently fatal w/o
treatment or vaccines. Few labs are at this level
-BSL-3 and full biohazard suit, shower on exit, lab must have own air supply
(only 13 in USA)
-ex. Ebola and Marburg viruses
Levels of Clean in the Clinic: Critical
Chapter 13 Microbiology UTA Tholen
Questions and Correct Answers/ Latest
Update / Already Graded
Main goal to controlling microbial growth
Ans: reduce microbial load and reduce infection/contamination
Sterilization
Ans: removal and killing of ALL microbes from fomite (inanimate
object)/organism
Disinfection
Ans: inactivation/kill of microbes on fomites
Sanitization
Ans: decrease of microbial load (amt. of microbes)
Biological Safety Level
,2 | Page
Ans: Levels of cleanliness assigned to labs; CDC, NIH, WHO established 4
levels
BSL-1
Ans: microbes aren't known to cause disease in healthy host and pose
minimal risk to workers and the environment
-sink for handwashing, door to close off lab (ex. freshman bio labs)
-ex. non-pathogenic strains of Escherichia coli
BSL-2
Ans: microbes are typically indigenous and are associated with diseases
of varying severity; they pose moderate risk to workers and the
environment
-BSL-1 and PPE, self-closing doors, eyewash station, autoclave, or
sterilization method (ex. Micro labs)
-ex. Staphylococcus aureus
, 3 | Page
BSL-3
Ans: microbes are indigenous/exotic and cause lethal diseases thru
respiratory transmission
-BSL-2 and respirator, bio safety cabinets, hands-free wash sink, two sets of
doors (ex. none at UTA)
-ex. Mycobacterium tuberculosis
BSL-4
Ans: - Microbes are dangerous & exotic, posing a
high risk of aerosol‐transmitted infections, which are frequently fatal w/o
treatment or vaccines. Few labs are at this level
-BSL-3 and full biohazard suit, shower on exit, lab must have own air supply
(only 13 in USA)
-ex. Ebola and Marburg viruses
Levels of Clean in the Clinic: Critical
