BIO1610 GENETICS EXAM NEWEST ACTUAL EXAM WITH
COMPLETE QUESTIONS AND CORRECT VERIFIED
ANSWERS(DETAILED ANSWERS) ALREADY GRADED A+ 100%
GUARANTEED TO PASS CONCEPTS!!!
True or false? The Taq enzyme is a type of DNA polymerase that allows
researchers to separate the DNA strands during the annealing step of the PCR
cycle without destroying the polymerase. - CORRECT ANSWER-False.
Taq polymerase is derived from a species of bacteria living in hot springs, which
makes it stable at the high temperature required for the denaturation step of
PCR.
How many DNA molecules would there be after four rounds of PCR if the initial
reaction mixture contained two molecules? - CORRECT ANSWER-32
During which step in the PCR cycle are nucleotides used?
-annealing
-extension
-denaturation
-all of the above - CORRECT ANSWER-Extension
,During which step in the PCR cycle do primers form bonds with a single-stranded
template?
-annealing
-extension
-denaturation
-all of the above - CORRECT ANSWER-annealing
Archaeologists unearthed a human skull with a small dried fragment of the scalp
still attached. They extracted a tiny amount of DNA from the scalp tissue. How
could they obtain sufficient DNA for an analysis of the ancient human's genes? -
CORRECT ANSWER-PCR
A paleontologist has recovered a bit of tissue from the 400-year-old preserved
skin of an extinct dodo (a bird). To compare a specific region of the DNADNA from
the sample with DNADNA from living birds, which of the following would be most
useful for increasing the amount of dodo DNADNA available for testing? -
CORRECT ANSWER-PCR
Which of the following reasons best explains why sequencing an entire genome,
such as that of C. elegans, a nematode, is important for genetic research? -
CORRECT ANSWER-A sequence that is found to have a particular function in the
nematode is likely to have a closely related function in vertebrates.
What information can not be obtained from the sequence of a gene?
-Relationship between two species
,-if gene is methylated
-effects of mutation on gene function
-amino acid sequence of the protein - CORRECT ANSWER-if the gene is
methylated
What is the polymerase chain reaction (PCR)? - CORRECT ANSWER-A method to
amplify a fragment of DNA
True or false? Comparison of the sequences of the same gene across species can
give some insight into the existence of a common ancestor with that gene. -
CORRECT ANSWER-True
Pax-6 is a gene involved in eye formation that has homologues in invertebrates
and vertebrates. The fact that expressing the mouse form of Pax-6 in a fruit fly
leads to a compound fly eye (not a single lens mouse eye) suggests which of the
following characteristics of this gene? - CORRECT ANSWER-Pax-6 gene is highly
conserved and shows shared evolutionary ancestry.
PCR and cellular DNA replication share which of the following characteristics?
-requires DNA primer to begin extension
-require helicase to separate DNA template strands
-form Okazaki fragments
-extends new strand in 5' to 3' direction - CORRECT ANSWER-extend new strand
in 5 to 3 direction
, Submit
What information is critical to the success of polymerase chain reaction (PCR)?
-The sequence of restriction-enzyme recognition sites in the DNA to be amplified
and in the plasmid where the amplified DNA fragment will be cloned must be
known.
-The complete DNA sequence of the DNA to be amplified must be known.
-The sequence of restriction-enzyme recognition sites in the DNA to be amplified
must be known.
-The DNA sequence of the ends of the DNA to be amplified must be known. -
CORRECT ANSWER-The DNA sequence of the ends of the DNA to be amplified
must be known.
cDNA is synthesized from what molecule, and using an enzyme known as what? -
CORRECT ANSWER-mRNA, reverse transcriptase
Which of the following statements about CRISPR-Cas9 is NOT correct? -
CORRECT ANSWER-CRISPR-Cas9 can be used to silence, or knock out, the
expression of a gene by destroying its mRNA transcripts or preventing their
translation.
Which of the following outcomes could be achieved using RNAi methodology? -
CORRECT ANSWER-reduction in protein produced from the gene of interest
COMPLETE QUESTIONS AND CORRECT VERIFIED
ANSWERS(DETAILED ANSWERS) ALREADY GRADED A+ 100%
GUARANTEED TO PASS CONCEPTS!!!
True or false? The Taq enzyme is a type of DNA polymerase that allows
researchers to separate the DNA strands during the annealing step of the PCR
cycle without destroying the polymerase. - CORRECT ANSWER-False.
Taq polymerase is derived from a species of bacteria living in hot springs, which
makes it stable at the high temperature required for the denaturation step of
PCR.
How many DNA molecules would there be after four rounds of PCR if the initial
reaction mixture contained two molecules? - CORRECT ANSWER-32
During which step in the PCR cycle are nucleotides used?
-annealing
-extension
-denaturation
-all of the above - CORRECT ANSWER-Extension
,During which step in the PCR cycle do primers form bonds with a single-stranded
template?
-annealing
-extension
-denaturation
-all of the above - CORRECT ANSWER-annealing
Archaeologists unearthed a human skull with a small dried fragment of the scalp
still attached. They extracted a tiny amount of DNA from the scalp tissue. How
could they obtain sufficient DNA for an analysis of the ancient human's genes? -
CORRECT ANSWER-PCR
A paleontologist has recovered a bit of tissue from the 400-year-old preserved
skin of an extinct dodo (a bird). To compare a specific region of the DNADNA from
the sample with DNADNA from living birds, which of the following would be most
useful for increasing the amount of dodo DNADNA available for testing? -
CORRECT ANSWER-PCR
Which of the following reasons best explains why sequencing an entire genome,
such as that of C. elegans, a nematode, is important for genetic research? -
CORRECT ANSWER-A sequence that is found to have a particular function in the
nematode is likely to have a closely related function in vertebrates.
What information can not be obtained from the sequence of a gene?
-Relationship between two species
,-if gene is methylated
-effects of mutation on gene function
-amino acid sequence of the protein - CORRECT ANSWER-if the gene is
methylated
What is the polymerase chain reaction (PCR)? - CORRECT ANSWER-A method to
amplify a fragment of DNA
True or false? Comparison of the sequences of the same gene across species can
give some insight into the existence of a common ancestor with that gene. -
CORRECT ANSWER-True
Pax-6 is a gene involved in eye formation that has homologues in invertebrates
and vertebrates. The fact that expressing the mouse form of Pax-6 in a fruit fly
leads to a compound fly eye (not a single lens mouse eye) suggests which of the
following characteristics of this gene? - CORRECT ANSWER-Pax-6 gene is highly
conserved and shows shared evolutionary ancestry.
PCR and cellular DNA replication share which of the following characteristics?
-requires DNA primer to begin extension
-require helicase to separate DNA template strands
-form Okazaki fragments
-extends new strand in 5' to 3' direction - CORRECT ANSWER-extend new strand
in 5 to 3 direction
, Submit
What information is critical to the success of polymerase chain reaction (PCR)?
-The sequence of restriction-enzyme recognition sites in the DNA to be amplified
and in the plasmid where the amplified DNA fragment will be cloned must be
known.
-The complete DNA sequence of the DNA to be amplified must be known.
-The sequence of restriction-enzyme recognition sites in the DNA to be amplified
must be known.
-The DNA sequence of the ends of the DNA to be amplified must be known. -
CORRECT ANSWER-The DNA sequence of the ends of the DNA to be amplified
must be known.
cDNA is synthesized from what molecule, and using an enzyme known as what? -
CORRECT ANSWER-mRNA, reverse transcriptase
Which of the following statements about CRISPR-Cas9 is NOT correct? -
CORRECT ANSWER-CRISPR-Cas9 can be used to silence, or knock out, the
expression of a gene by destroying its mRNA transcripts or preventing their
translation.
Which of the following outcomes could be achieved using RNAi methodology? -
CORRECT ANSWER-reduction in protein produced from the gene of interest
