BIO 322 – 2026/2027 Revised Questions
(Graded A+) 100% Correct Answers
You think you have determined where the promoter/enhancer for Sox9 is and want to find all of the
tissues that express the Sox9 gene during embryogenesis. Which of the following would be the best
method to use to determine this?
A. Use microarray of Sox9 embryo RNAB. Carry out Sox9 specific RNA interference. Make a transgene
expressing GFP with the Sox9 promoter/enhanced. Make a knockout of the Sox9 gene using a
Cre/Lox approach - C. Make a transgene expressing GFP with the Sox9 promoter/enhancer
You want to knock out the gene Herculin in chickens, but there are no chicken ES cells. What is
another way that you could block Herculin expression?
A. Make a transgene of the cDNA for the geneB. Design an siRNA specific to the Herculin proteinC.
Do a chicken microarrayD. Design an siRNA specific to the Herculin mRNA - D. Design an siRNA
specific to the Herculin mRNA
Which of the following describes the elements that are required to construct a minimally functional
transgene? - It has a promoter, intron, protein coding sequence (reporter gene) and transcriptional
STOP
If you took skin fibroblasts and increased expression of Oct4 (Pou5tf), Myc, Klf4, and Sox2, what
would you be converting the fibroblasts into? - Induced pluripotent stem cells (IPSc)
You have made a targeting vector to knock out Gene X. What is the next step in making a knock out
mouse? - Electroporate embryonic stem cells (ES) with the linearized vector, then select for those
cells that are correctly targeted.
What will be the result of thymidine kinase gene (TK) being incorporated into an ES cell's genome? -
it will make cells sensitive to gancyclovir
In order to knock out Gene X how would the targeting vector need to integrate into the genome? - It
will recombine homologously into the gene X locus.
The 2007 Nobel Prize in Physiology and Medicine was awarded to three researchers for introducing
site-specific genetic modifications into ES cells. What was the biggest advantage of this technology
when compared to previous transgenic approaches?
(Graded A+) 100% Correct Answers
You think you have determined where the promoter/enhancer for Sox9 is and want to find all of the
tissues that express the Sox9 gene during embryogenesis. Which of the following would be the best
method to use to determine this?
A. Use microarray of Sox9 embryo RNAB. Carry out Sox9 specific RNA interference. Make a transgene
expressing GFP with the Sox9 promoter/enhanced. Make a knockout of the Sox9 gene using a
Cre/Lox approach - C. Make a transgene expressing GFP with the Sox9 promoter/enhancer
You want to knock out the gene Herculin in chickens, but there are no chicken ES cells. What is
another way that you could block Herculin expression?
A. Make a transgene of the cDNA for the geneB. Design an siRNA specific to the Herculin proteinC.
Do a chicken microarrayD. Design an siRNA specific to the Herculin mRNA - D. Design an siRNA
specific to the Herculin mRNA
Which of the following describes the elements that are required to construct a minimally functional
transgene? - It has a promoter, intron, protein coding sequence (reporter gene) and transcriptional
STOP
If you took skin fibroblasts and increased expression of Oct4 (Pou5tf), Myc, Klf4, and Sox2, what
would you be converting the fibroblasts into? - Induced pluripotent stem cells (IPSc)
You have made a targeting vector to knock out Gene X. What is the next step in making a knock out
mouse? - Electroporate embryonic stem cells (ES) with the linearized vector, then select for those
cells that are correctly targeted.
What will be the result of thymidine kinase gene (TK) being incorporated into an ES cell's genome? -
it will make cells sensitive to gancyclovir
In order to knock out Gene X how would the targeting vector need to integrate into the genome? - It
will recombine homologously into the gene X locus.
The 2007 Nobel Prize in Physiology and Medicine was awarded to three researchers for introducing
site-specific genetic modifications into ES cells. What was the biggest advantage of this technology
when compared to previous transgenic approaches?
