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QBM Exam 1 Practice Test questions with verified answers.

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QBM Exam 1 Practice Test questions with
verified answers
Oswald Avery showed that Ans✓✓-DNA is responsible for bacterial
transformations.


Which of the following refers to manipulating a piece of DNA and putting it into a
plasmid for the purposes of gene studies, protein expression, and gene therapy?
Ans✓✓-Recombinant DNA technology


Employers must inform employees and train them in the safe handling potentially
harmful chemicals. This is required by Ans✓✓-OSHA


Research labs that handle pathogenic organisms for which there is no vaccine that
can cause diseases which are generally untreable and fatal are assigned a rating of
Ans✓✓-BSL-4


Where should you open bottles that contain volatile chemicals such as HCl or
SDS? Ans✓✓-In the fume hood.


Which type of gloves offer the least amount of protection from chemical/
biological hazards? Ans✓✓-Plastic


A device used to clean glassware and sterilize media using heat and pressure is
a(n) Ans✓✓-autoclave

, The least accurate way to transfer a specific volume would be with a Ans✓✓-
Pasteur (transfer) pipette


A Mohr pipette has a thinner tip, which is beneficial when using the pipette to
Ans✓✓-dispense specific volumes several times from a single aspiration


Some tips have cotton swabs in them to Ans✓✓-prevent contamination of the
pipettes and samples


You have a 1.5 ml tube of frozen enzyme. Upon removing it from the freezer you
should thaw it and spin it down briefly in a centrifuge. What is the purpose of
this? Ans✓✓-To remove any liquid from inside the cap


Why are most centrifuges refrigerated? Ans✓✓-To prevent protein degradation


A type of centrifugation used in the lab to rapidly collect samples in 1.5 ml tubes
is Ans✓✓-small bench top centrifugation.


A protocol you are following says to spin a sample in a Sorvall SA-600 at 10,000
rpm. You are using a Sorvall SS-34 rotor, which is much smaller. What must you
do? Ans✓✓-Calculate the relative centrifugal field of the same SA-600 to
determine what rpm to use in the SS-34.


When using a pipette, the greatest accuracy is achieved by Ans✓✓-holding the
pipette perfectly vertical


with regards to potential destructive ability, which type of centrifuge is most
dangerous and must be balanced within 0.1g? Ans✓✓-Ultracentrifuge

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