ArianaNicoleMendota 2256731
15
october 2nd 2024 selected themixculturefrom our TA I pickedthe
tube 15 andtheninoculated aseptically tothe nutrientagarplate I used
asterileloop passed it throughthe flame and transferred it usingthequadrant
method Theplatewas labeled anddividedinto sections thefinalquadrantwas
streakedmoreseparate inordertoobtained theisolated coloniesfromboth bacteria
Platewasinoculated at 30C andwillbeanalytednextlab
October7th2024 plate in the
Today we will observethemorphologyofthecolor oneswere
stereoscope itwaseasy toseetheircircularshapeandopaque
darkerthan others both were elevated and had a smoothtexture After I
identify the bacteria Placedtheslides in the
preformed the gram stain test to
identifyingthepinkone
tense until
observe in 404objective
microscopeand a nutrientagarplate streaking
Then I subcultured the gram negative unto separate
in quadrantmethodandincubatingagain in 30 C for 48hours
October 9th 2024 atthismoment I'mgoingtoconfirm I havea gramnegativeso
newsubculture
i'll proceed torepeatthe steps and confirmit I'll betest onemy
testing
beforeany biochemicaltests I didthe gram stain
moretime
shaped and I used
this time allmybacteria was pink in rodof
immersion oil toappreciate the shape all colonywas roundand
dispersedwell
, proceed to dothe gramstain
procedure
October 14th2024 I
resultswere contaminated in someareas
onemoretime Howevermy
I saw purplebacteria ontopof pinkspots I needed toinoculatefrommy
specificcolony I didthetestaroundthree
plateagainbeing cautius grabbingthe
took me a lot of triesuntil I
timesuntil I didnothaveanycontamination Itbiochemical tests for48hours in
proceeded to inoculate it on the
was and
sure
pictures to comparethecontrols in the
future
a temperature of 30 C I took
october16th2024 Today Ihadbacktheresults ofmy biochemical tests so
isgram
so I inoculated thesubculture thatVoges
isolatedthen I used a wireloop
andasepticallyproceed to dothe
Proskaeur testandmy methylred
ositivewhichwas animmediate change Then
test myresultforMRwasPegative my
for VDtestwas productAfter analyzingmy results
my organism does
myresult
not haveacetoin as anend
es indole
a Mastskitsfor
that
withha
81hm in andpheed
will be incubated for48hours at 30C
Hasformation and motility They
the unknown bacteria I'll proceed towork
the tubes with
Afterinoculating
handout 8
onmylab
October 23rd 2024 lastday I'll
workon the unknownproject I'll
and sulfur
Indole negative
record theresultswhichwas ureanegative lactoseand
Ithink bacteria is providencia alcalifaciens sincethe
formation negative my
glucosewere K whichmeansithaddegradationofpeptone
my first subculture was streakedcorrectly but my second one
was not completely I couldn't
see too muchIsolated colonies
worked with the first one and did
in my second one so I to
one I needed use three plates to have
a third and identify them better
a better observation did not
have had happen if I accidently
contamination may have grabbed to much bacteria
I may
inoculate them correctlyI needed to try several times I was
which wasin athereason
rest of laboratory techniques
as mantaining
successful
everything aseptically
15
october 2nd 2024 selected themixculturefrom our TA I pickedthe
tube 15 andtheninoculated aseptically tothe nutrientagarplate I used
asterileloop passed it throughthe flame and transferred it usingthequadrant
method Theplatewas labeled anddividedinto sections thefinalquadrantwas
streakedmoreseparate inordertoobtained theisolated coloniesfromboth bacteria
Platewasinoculated at 30C andwillbeanalytednextlab
October7th2024 plate in the
Today we will observethemorphologyofthecolor oneswere
stereoscope itwaseasy toseetheircircularshapeandopaque
darkerthan others both were elevated and had a smoothtexture After I
identify the bacteria Placedtheslides in the
preformed the gram stain test to
identifyingthepinkone
tense until
observe in 404objective
microscopeand a nutrientagarplate streaking
Then I subcultured the gram negative unto separate
in quadrantmethodandincubatingagain in 30 C for 48hours
October 9th 2024 atthismoment I'mgoingtoconfirm I havea gramnegativeso
newsubculture
i'll proceed torepeatthe steps and confirmit I'll betest onemy
testing
beforeany biochemicaltests I didthe gram stain
moretime
shaped and I used
this time allmybacteria was pink in rodof
immersion oil toappreciate the shape all colonywas roundand
dispersedwell
, proceed to dothe gramstain
procedure
October 14th2024 I
resultswere contaminated in someareas
onemoretime Howevermy
I saw purplebacteria ontopof pinkspots I needed toinoculatefrommy
specificcolony I didthetestaroundthree
plateagainbeing cautius grabbingthe
took me a lot of triesuntil I
timesuntil I didnothaveanycontamination Itbiochemical tests for48hours in
proceeded to inoculate it on the
was and
sure
pictures to comparethecontrols in the
future
a temperature of 30 C I took
october16th2024 Today Ihadbacktheresults ofmy biochemical tests so
isgram
so I inoculated thesubculture thatVoges
isolatedthen I used a wireloop
andasepticallyproceed to dothe
Proskaeur testandmy methylred
ositivewhichwas animmediate change Then
test myresultforMRwasPegative my
for VDtestwas productAfter analyzingmy results
my organism does
myresult
not haveacetoin as anend
es indole
a Mastskitsfor
that
withha
81hm in andpheed
will be incubated for48hours at 30C
Hasformation and motility They
the unknown bacteria I'll proceed towork
the tubes with
Afterinoculating
handout 8
onmylab
October 23rd 2024 lastday I'll
workon the unknownproject I'll
and sulfur
Indole negative
record theresultswhichwas ureanegative lactoseand
Ithink bacteria is providencia alcalifaciens sincethe
formation negative my
glucosewere K whichmeansithaddegradationofpeptone
my first subculture was streakedcorrectly but my second one
was not completely I couldn't
see too muchIsolated colonies
worked with the first one and did
in my second one so I to
one I needed use three plates to have
a third and identify them better
a better observation did not
have had happen if I accidently
contamination may have grabbed to much bacteria
I may
inoculate them correctlyI needed to try several times I was
which wasin athereason
rest of laboratory techniques
as mantaining
successful
everything aseptically
