Microbiology Lab Practical 1 Gacura
Questions and Answers 100% Pass
Primary containment - ✔✔protection of the lab personnel and the lab environment
Secondary Containment - ✔✔protection of the outside lab environment from exposure
to infectious organisms
Disposal of biohazards - ✔✔Place biohazard material in marked bag or container; must
be tagged or color coded
Disposal of Glass waste - ✔✔can be washed and reused after autoclaving (Kills station)
Disposal of plastic waste - ✔✔Melt in autoclave to dispose after decontaminated
Office hours and location - ✔✔Zurn 207 Monday/Wednesday 1-2:30
Cidal - ✔✔killing
bactericidal
Static - ✔✔Halts growth
Katelyn Whitman, All Rights Reserved © 2025 1
, bacteriostatic
Resident Flora - ✔✔microorganisms that normally reside on locations (like skin)
Hard to get rid of
transient flora - ✔✔microbes that reside on the skin surface and are easily removed (not
typical residents on skin)- there from contamination
What type of agar do we use? - ✔✔Tryptic Soy agar
Colony - ✔✔visible cluster of microbes growing on solid media (1 cell asexually
reproduces until it's visible.
What temperature is an autoclave? - ✔✔121 degrees Celsius
What temp did we incubate the hand washing agar lab dish? - ✔✔37 degrees Celsius
(human body temp)
Inoculation loop - ✔✔Used to transfer microbiological live specimens under sterile
conditions
Nutrient broth - ✔✔liquid medium used to cultivate bacteria (it feeds bacteria)
What are the Eukaryotic super groups? - ✔✔Unikonta
Excavata
Katelyn Whitman, All Rights Reserved © 2025 2
Questions and Answers 100% Pass
Primary containment - ✔✔protection of the lab personnel and the lab environment
Secondary Containment - ✔✔protection of the outside lab environment from exposure
to infectious organisms
Disposal of biohazards - ✔✔Place biohazard material in marked bag or container; must
be tagged or color coded
Disposal of Glass waste - ✔✔can be washed and reused after autoclaving (Kills station)
Disposal of plastic waste - ✔✔Melt in autoclave to dispose after decontaminated
Office hours and location - ✔✔Zurn 207 Monday/Wednesday 1-2:30
Cidal - ✔✔killing
bactericidal
Static - ✔✔Halts growth
Katelyn Whitman, All Rights Reserved © 2025 1
, bacteriostatic
Resident Flora - ✔✔microorganisms that normally reside on locations (like skin)
Hard to get rid of
transient flora - ✔✔microbes that reside on the skin surface and are easily removed (not
typical residents on skin)- there from contamination
What type of agar do we use? - ✔✔Tryptic Soy agar
Colony - ✔✔visible cluster of microbes growing on solid media (1 cell asexually
reproduces until it's visible.
What temperature is an autoclave? - ✔✔121 degrees Celsius
What temp did we incubate the hand washing agar lab dish? - ✔✔37 degrees Celsius
(human body temp)
Inoculation loop - ✔✔Used to transfer microbiological live specimens under sterile
conditions
Nutrient broth - ✔✔liquid medium used to cultivate bacteria (it feeds bacteria)
What are the Eukaryotic super groups? - ✔✔Unikonta
Excavata
Katelyn Whitman, All Rights Reserved © 2025 2
