Microbiology Lab Quiz 1 Questions
and Answers 100% Pass
Proper use of the microscope - ✔✔all specimens must be viewed under cover glass,
focus with scanning objectives first, use course adjustment knob, once in focus, use the
fine focusing knob (when viewing with low or high power objectives)
Depth of Field - ✔✔refers to the thickness of the plane of focus
Field of View - ✔✔area that is visible when using the microscope
Why use a microscope? - ✔✔To view and study the microscopic world of organisms
and objects less than 5mm in size that cohabitate with us on our globe.
Ocular lens (location and job) - ✔✔Area you place your eyes to see in scope (top of the
tube) - magnifies the image formed by the objective lens
Objective lenses (location and job) - ✔✔the lenses right above where the sample is
placed to be viewed. Can be changed to different magnifications (scanning, low, high). -
magnifies the object
Specimen holder (location and job) - ✔✔located right under the objective lenses - holds
specimen in place
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, Mechanical Stage (location and job) - ✔✔platform that you place your slides on - holds
them in place with help of specimen holders and can be be moved up and down or left
to right
Light intensity control (location and job) - ✔✔usually by or with the main power
switch. Controls the intensity of the light
x-y axis control knob (location and job) - ✔✔attached to the stage (mechanical) - moves
the stage along the x/y axis. One knob for back/front, one knob for left/right
Course focusing knob (location and job) - ✔✔located on side of microscope usually with
fine focus knob - rough focus knob only used with high power objective
Fine focusing knob (location and job) - ✔✔located on side of microscope usually with
coarse focus knob - focus knob used to fine tune the focus on the specimen
Iris diaphragm (location and job) - ✔✔under the condenser and above the light source -
controls the amount of light that enters the condenser.
Condenser (location and job) - ✔✔lens mounted below the stage and above iris
diaphram - used to focus the light on a small area above the stage
Light source (location and job) - ✔✔mounted under the condenser (maybe in/under the
base) - the light source for the microscope
Katelyn Whitman, All Rights Reserved © 2025 2
and Answers 100% Pass
Proper use of the microscope - ✔✔all specimens must be viewed under cover glass,
focus with scanning objectives first, use course adjustment knob, once in focus, use the
fine focusing knob (when viewing with low or high power objectives)
Depth of Field - ✔✔refers to the thickness of the plane of focus
Field of View - ✔✔area that is visible when using the microscope
Why use a microscope? - ✔✔To view and study the microscopic world of organisms
and objects less than 5mm in size that cohabitate with us on our globe.
Ocular lens (location and job) - ✔✔Area you place your eyes to see in scope (top of the
tube) - magnifies the image formed by the objective lens
Objective lenses (location and job) - ✔✔the lenses right above where the sample is
placed to be viewed. Can be changed to different magnifications (scanning, low, high). -
magnifies the object
Specimen holder (location and job) - ✔✔located right under the objective lenses - holds
specimen in place
Katelyn Whitman, All Rights Reserved © 2025 1
, Mechanical Stage (location and job) - ✔✔platform that you place your slides on - holds
them in place with help of specimen holders and can be be moved up and down or left
to right
Light intensity control (location and job) - ✔✔usually by or with the main power
switch. Controls the intensity of the light
x-y axis control knob (location and job) - ✔✔attached to the stage (mechanical) - moves
the stage along the x/y axis. One knob for back/front, one knob for left/right
Course focusing knob (location and job) - ✔✔located on side of microscope usually with
fine focus knob - rough focus knob only used with high power objective
Fine focusing knob (location and job) - ✔✔located on side of microscope usually with
coarse focus knob - focus knob used to fine tune the focus on the specimen
Iris diaphragm (location and job) - ✔✔under the condenser and above the light source -
controls the amount of light that enters the condenser.
Condenser (location and job) - ✔✔lens mounted below the stage and above iris
diaphram - used to focus the light on a small area above the stage
Light source (location and job) - ✔✔mounted under the condenser (maybe in/under the
base) - the light source for the microscope
Katelyn Whitman, All Rights Reserved © 2025 2
