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Microbiology Lab – Stains Exam Questions and Answers 100% Pass

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Microbiology Lab – Stains Exam Questions and Answers 100% Pass Simple stain - -Add color to cells for viewing -Highlight entire organism's shape and basic structures -Includes: Methylene blue Safranin Crystal violet Carbolfuchsin Negative stain - -Enhances viewing of morphology, capsules -Bacteria are live -No heat fixing! (no denaturing) -Nigrosin ink -View under oil 2Katelyn Whitman, All Rights Reserved © 2025 -Anionic (acidic) simple stain Gram stain - -Determines cell wall type (gram positive or negative) -Differential stain Gram stain procedure - 1. Crystal violet 2. Iodine (mordant) 3. Alcohol (decolorizer) 4. Safranin (counterstain) 5. Observe under oil Mordant - gram stain - -Increases intensity of crystal violet -Iodine enters cell forms crystal violet-iodine com

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Microbiology Lab

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Microbiology Lab

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Institution: Microbiology Lab
Course: Microbiology Lab

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Uploaded on: January 17, 2025
Number of pages: 4
Written in: 2024/2025
Type: Exam (elaborations)
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microbiology lab stains exam questions and answe

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Microbiology Lab – Stains Exam
Questions and Answers 100% Pass

Simple stain - ✔✔-Add color to cells for viewing

-Highlight entire organism's shape and basic structures

-Includes:

Methylene blue

Safranin

Crystal violet

Carbolfuchsin

Negative stain - ✔✔-Enhances viewing of morphology, capsules

-Bacteria are live

-No heat fixing! (no denaturing)

-Nigrosin ink

-View under oil

Katelyn Whitman, All Rights Reserved © 2025 1

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Microbiology Lab – Stains Exam Questions and Answers 100% Pass

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