Microbiology Lab Practical 1 Study
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Illuminator - ✔✔Filter for shorter λ
Condenser microscope - ✔✔Focuses light into specimen
Diaphragm (iris) - ✔✔Controls amount of light to specimen
Objective lenses at nosepiece - ✔✔Magnifies image 10, 40, or 100 times
Ocular lens - ✔✔magnifies image 10X
Course adjustment - ✔✔focuses specimen in a broad way
Fine adjustment - ✔✔fine focuses specimen
Stage - ✔✔a platform used to hold the slide containing the specimen
Arm - ✔✔serves as a handle for carrying microscope
Mechanical stage adjuster - ✔✔moves stage forward and back
Stage clip - ✔✔Holds microscope slide in position
Katelyn Whitman, All Rights Reserved © 2025 1
, Magnification - ✔✔increase in apparent size
Resolution - ✔✔clarity
Oil has the __________ refractive index as glass - ✔✔same
This is the part of the microscope that should not be
touched under high power. - ✔✔the course adjustment knob
How does the light intensity change when a student goes from a low power objective to
a high power objective? - ✔✔The field of vision narrowed
Which lens do you use oil with? - ✔✔100X lens
How does immersion oil work to enhance resolution? - ✔✔Same refractive index as
glass; prevents loss of light to air
What is the maximum resolution of your microscope (in micrometers) or how far apart
do two organisms have to be from one another in order to see them as 2 different
organisms under the scope? - ✔✔.2 um with 100X
What is the total magnification of your scope when you use each lens? - ✔✔ocular lens
magnification times the magnification of the objective lens 10X x 40X, 100X, 10X
What do you have to do to your microscope before you put it away? - ✔✔lower the
stage
Katelyn Whitman, All Rights Reserved © 2025 2
guide Questions and Answers 100%
Pass
Illuminator - ✔✔Filter for shorter λ
Condenser microscope - ✔✔Focuses light into specimen
Diaphragm (iris) - ✔✔Controls amount of light to specimen
Objective lenses at nosepiece - ✔✔Magnifies image 10, 40, or 100 times
Ocular lens - ✔✔magnifies image 10X
Course adjustment - ✔✔focuses specimen in a broad way
Fine adjustment - ✔✔fine focuses specimen
Stage - ✔✔a platform used to hold the slide containing the specimen
Arm - ✔✔serves as a handle for carrying microscope
Mechanical stage adjuster - ✔✔moves stage forward and back
Stage clip - ✔✔Holds microscope slide in position
Katelyn Whitman, All Rights Reserved © 2025 1
, Magnification - ✔✔increase in apparent size
Resolution - ✔✔clarity
Oil has the __________ refractive index as glass - ✔✔same
This is the part of the microscope that should not be
touched under high power. - ✔✔the course adjustment knob
How does the light intensity change when a student goes from a low power objective to
a high power objective? - ✔✔The field of vision narrowed
Which lens do you use oil with? - ✔✔100X lens
How does immersion oil work to enhance resolution? - ✔✔Same refractive index as
glass; prevents loss of light to air
What is the maximum resolution of your microscope (in micrometers) or how far apart
do two organisms have to be from one another in order to see them as 2 different
organisms under the scope? - ✔✔.2 um with 100X
What is the total magnification of your scope when you use each lens? - ✔✔ocular lens
magnification times the magnification of the objective lens 10X x 40X, 100X, 10X
What do you have to do to your microscope before you put it away? - ✔✔lower the
stage
Katelyn Whitman, All Rights Reserved © 2025 2
