Microbiology lab practical exam 1 Questions and Answers 100% Pass

Microbiology lab practical exam 1
Questions and Answers 100% Pass


Wet mount steps: - ✔✔1.water


2. specimen


3. Cover slip over specimen


hanging drop steps: - ✔✔1. Vaseline around cover slip


2. micro organism in center of cover slip


3. place concave slide on cover slip


Gram stain steps: - ✔✔1. Crystal Violet


2. Gram's Iodine


3. Ethyl Alcohol


4. Safranin Red


negative stain steps: - ✔✔1. nigrosin


2.heat loop




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, 3.bacteria


4. slide to spread mixture


5. air dry


acid-fast stain steps: - ✔✔1. carbolfuchsin


2. acid alcohol


3. methylene blue


4. wash & dry


simple stain steps - ✔✔1. fixed smear slide


2. dyes


3. rinse & dry


nutrient broth - ✔✔-liquid media used to grow bacteria


agar - ✔✔-used to culture microorganisms


candle jar - ✔✔-used for anaerobic organisms


Petri dish - ✔✔-Used to culture or observe bacteria growth


slant - ✔✔-a medium for which bacteria can grow on.


E.coli Gram stain - ✔✔red colored rods




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