Microbiology Lab Exam 1 Note Cards
Exam Questions and Answers 100% Pass
when viewing a smear with the oil immersion lens the final magnification is -
✔✔1000x;10x ocular x 100X objective
what is the purpose of heat fixing - ✔✔kill the bacteria, adhere to glass anddenature the
proteins of bacteria
name oneof the stains or regents used in the gram stain procedure - ✔✔gram crystal
violet, gram iodine, gram sfaranin, 95% ethanol
why are aseptic techniques used in the lab? - ✔✔to ensure that we do not contaminate
what we are working with as as to ensue that we do not acciently inoculate ouselves.
example of aseptic techniques are flaming the loop/needle sterilizing it, breifly flaming
the test tube before and after obtaining the sample.
the gram reaction is due to differences in the structure of wat part of the microbe -
✔✔its cell wall, specifically peptidoglycan, gram positive bacteria have thicker
peptidoglycan layer compared to GN bacteria.
Name 2 inoculating tools you will be using in this lab - ✔✔loop, needle
Katelyn Whitman, All Rights Reserved © 2025 1
, what color is a gram positive microbe - ✔✔purple
waht is the function of the ethanol in the gram stain procedure - ✔✔decolorize
name one of the strains in the acid fast stain - ✔✔cold kinyoun, carbol fuschsin,
methylen blue
name one of the stains in the endospore stain - ✔✔malachite green, safranin
what is the purpose of the acid-fast stain - ✔✔the purpose of the acid-fast stain is to
differentiate between the myobactriace family of bacteria from all other bacteria
what color does na acid-fast microbe stain with acd-fast stain - ✔✔red
what is the function of agarin the media - ✔✔agar allows media to remain solid at room
temperature
what is the difference between broth and agar based media - ✔✔broth is liquid media at
room temperature, agar media is solid at room temp.
what is the funciton of media - ✔✔media is used to provide nutrients to a microbe as
well as a growth medium
what is the name of the plate inoculation technique - ✔✔quadrant steak plate
Katelyn Whitman, All Rights Reserved © 2025 2
Exam Questions and Answers 100% Pass
when viewing a smear with the oil immersion lens the final magnification is -
✔✔1000x;10x ocular x 100X objective
what is the purpose of heat fixing - ✔✔kill the bacteria, adhere to glass anddenature the
proteins of bacteria
name oneof the stains or regents used in the gram stain procedure - ✔✔gram crystal
violet, gram iodine, gram sfaranin, 95% ethanol
why are aseptic techniques used in the lab? - ✔✔to ensure that we do not contaminate
what we are working with as as to ensue that we do not acciently inoculate ouselves.
example of aseptic techniques are flaming the loop/needle sterilizing it, breifly flaming
the test tube before and after obtaining the sample.
the gram reaction is due to differences in the structure of wat part of the microbe -
✔✔its cell wall, specifically peptidoglycan, gram positive bacteria have thicker
peptidoglycan layer compared to GN bacteria.
Name 2 inoculating tools you will be using in this lab - ✔✔loop, needle
Katelyn Whitman, All Rights Reserved © 2025 1
, what color is a gram positive microbe - ✔✔purple
waht is the function of the ethanol in the gram stain procedure - ✔✔decolorize
name one of the strains in the acid fast stain - ✔✔cold kinyoun, carbol fuschsin,
methylen blue
name one of the stains in the endospore stain - ✔✔malachite green, safranin
what is the purpose of the acid-fast stain - ✔✔the purpose of the acid-fast stain is to
differentiate between the myobactriace family of bacteria from all other bacteria
what color does na acid-fast microbe stain with acd-fast stain - ✔✔red
what is the function of agarin the media - ✔✔agar allows media to remain solid at room
temperature
what is the difference between broth and agar based media - ✔✔broth is liquid media at
room temperature, agar media is solid at room temp.
what is the funciton of media - ✔✔media is used to provide nutrients to a microbe as
well as a growth medium
what is the name of the plate inoculation technique - ✔✔quadrant steak plate
Katelyn Whitman, All Rights Reserved © 2025 2
