Test Bank for Biochemistry
The Molecular Basis of Life
7th Edi�on By Trudy McKee,
James McKee
All Chapters Arranged
Reverse: Chapter 19 to 1.
, CHAPTER NINETEEN
PROTEIN SYNTHESIS
1)The Shine-Dalgarno sequence is a
A) Prokaryotic mRNA sequence that binds IF-3
B) Prokaryotic mRNA sequence that binds 16S rRNA of the small ribosomal subunit
C) Prokaryotic mRNA sequence that binds the 16S RNA of the large ribosomal
subunit
D) Eukaryotic rRNA sequence that binds the AUG codon of mRNA
E) None of the above are correct
Ans B
2) The initiation phase of prokaryotic translation ends as the GTP molecule bound to
________ is hydrolyzed to GDP and Pi
A) IF-1
B) IF-2
C) IF-3
D) IF-4
E) None of the above is correct
Ans B
3) During the elongation phase of prokaryotic translation the aminoacyl-tRNA must
first bind _________ before it can be positioned in the A site.
A) EF-Ts
B) EF-Tu
C) EF-Ts-GDP
D) EF-Ts-GTP
E) EF-Tu-GTP
Ans E
4)________ is an enzymatic activity that hydrolyses the bond linking the completed
polypeptide chain and the P site tRNA
A) RF-1
B) EF-G
C) Peptidyl transferase
D) Ribosomal esterase
E) Signal peptidase
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,Ans C
5) Prominent prokaryotic posttranslational modifications include all of the following
except __________.
A) Glycosylation
B) Methylation
C) Proteolytic processing
D) Prenylation
E) Phosphorylation
Ans D
6) In prokaryotes most of the control of protein synthesis occurs at the level of
______.
A) Translation
B) Transcription
C) Replication
D) Reverse transcription
E) Posttranslational modification
Ans B
7) The enzymatic activities coded by the lac operon include all of the following
except:
A) Thiogalactoside transacylase
B) Lactose transferase
Χ) -Galactosidase
D) Galactose permease
E) None of the above is correct
Ans B
8) Translocation in eukaryotes requires _____, which is a GTP binding protein.
A) eEF-1
B) eEF-2
C) eEF-3
D) eEF-4
E) None of the above is correct
Ans B
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, 9) In eukaryotic translation the 40S complex binds the 60S subunit to form the
_______ initiation complex.
A) 80S
B) 88S
C) 90S
D) 100S
E) 108S
Ans A
10) ________ provides the energy needed to move the ribosome along an mRNA.
A) ATP
B) GTP
C) UTP
D) TTP
E) All of the above are correct
Ans B
11) Polypeptides containing signal peptides are called ________.
A) Zymogens
B) Preproproteins
C) Proproteins
D) All of the above are correct
E) None of the above is correct
Ans B
12) Phosphorylated dolichol, a polyisoprenoid found in ER membrane, is involved in
A) Polypeptide synthesis
B) Proteolytic cleavage reactions
C) Lipophilic modification reactions
D) Hydroxylation reactions
E) Glycosylation reactions
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The Molecular Basis of Life
7th Edi�on By Trudy McKee,
James McKee
All Chapters Arranged
Reverse: Chapter 19 to 1.
, CHAPTER NINETEEN
PROTEIN SYNTHESIS
1)The Shine-Dalgarno sequence is a
A) Prokaryotic mRNA sequence that binds IF-3
B) Prokaryotic mRNA sequence that binds 16S rRNA of the small ribosomal subunit
C) Prokaryotic mRNA sequence that binds the 16S RNA of the large ribosomal
subunit
D) Eukaryotic rRNA sequence that binds the AUG codon of mRNA
E) None of the above are correct
Ans B
2) The initiation phase of prokaryotic translation ends as the GTP molecule bound to
________ is hydrolyzed to GDP and Pi
A) IF-1
B) IF-2
C) IF-3
D) IF-4
E) None of the above is correct
Ans B
3) During the elongation phase of prokaryotic translation the aminoacyl-tRNA must
first bind _________ before it can be positioned in the A site.
A) EF-Ts
B) EF-Tu
C) EF-Ts-GDP
D) EF-Ts-GTP
E) EF-Tu-GTP
Ans E
4)________ is an enzymatic activity that hydrolyses the bond linking the completed
polypeptide chain and the P site tRNA
A) RF-1
B) EF-G
C) Peptidyl transferase
D) Ribosomal esterase
E) Signal peptidase
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,Ans C
5) Prominent prokaryotic posttranslational modifications include all of the following
except __________.
A) Glycosylation
B) Methylation
C) Proteolytic processing
D) Prenylation
E) Phosphorylation
Ans D
6) In prokaryotes most of the control of protein synthesis occurs at the level of
______.
A) Translation
B) Transcription
C) Replication
D) Reverse transcription
E) Posttranslational modification
Ans B
7) The enzymatic activities coded by the lac operon include all of the following
except:
A) Thiogalactoside transacylase
B) Lactose transferase
Χ) -Galactosidase
D) Galactose permease
E) None of the above is correct
Ans B
8) Translocation in eukaryotes requires _____, which is a GTP binding protein.
A) eEF-1
B) eEF-2
C) eEF-3
D) eEF-4
E) None of the above is correct
Ans B
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, 9) In eukaryotic translation the 40S complex binds the 60S subunit to form the
_______ initiation complex.
A) 80S
B) 88S
C) 90S
D) 100S
E) 108S
Ans A
10) ________ provides the energy needed to move the ribosome along an mRNA.
A) ATP
B) GTP
C) UTP
D) TTP
E) All of the above are correct
Ans B
11) Polypeptides containing signal peptides are called ________.
A) Zymogens
B) Preproproteins
C) Proproteins
D) All of the above are correct
E) None of the above is correct
Ans B
12) Phosphorylated dolichol, a polyisoprenoid found in ER membrane, is involved in
A) Polypeptide synthesis
B) Proteolytic cleavage reactions
C) Lipophilic modification reactions
D) Hydroxylation reactions
E) Glycosylation reactions
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