ASCP CG Exam (Classic Cytogenetics)|
Questions with Answers
What are the basics of in situ hybridization? - -Pretreatment of slides with
acid, extraction of DNA with barium hydroxide, and incubation in 2xSSC to
promote denaturation.
- The following are indicators for amniocentesis except: - -A family history of
an autosomal recessive disorder.
- When a bone marrow sample cannot be delivered to the lab within 4 hrs,
the sample should: - -Be put in media and kept at room temp.
- A PB sample is clotted. What do you do? - -Break up the clot.
- All of the following information is required during the specimen set up
procedure except: - -Infectious agent.
- When should samples be logged in? - -As soon as they arrive in the
laboratory.
- How long do you wait before requesting an amniotic fluid if no cell
attachment is observed? - -10 days.
- All of the following will result in culture failure except: - -Transport at room
temp.
- Which cells would you want to use for long term serial cytogenetic studies?
- -Fibroblasts.
- The following enzymes can be used to dissociate chorionic villus samples
except for: - -RNAse.
- Which of the following is not a necessary ingredient which is added to
modified medium? - -Antibiotics.
- All of the following have buffering capability except: - -L-Glutamine.
- What type of media is necessary for the culture of Fragile X cases? - -Folic
acid deficient media.
- The most important reason why Biological Safety Caninets are used
instead of Horizontal Laminar Flow Hoods in the handling of human tissues
is: - -To protect the technologist who is processing the specimen.
, - In which of the following situations would one suspect bacterial
contamination? - -The culture media is turbid in appearance.
- Which of the following can be used for serial culture from amniotic fluid? -
-Fibroblasts.
- Which of the following chemicals is used for cryopreservation? - -Dimethyl
sulfoxide (DMSO).
- Adding the drug Colcemid to cell cultures can cause all of the following
phenomenons to occur except: - -Cell swelling.
- During slidemaking all of the following factors are important except for: - -
Slides are 1st being dipped in acetic acid.
- A good banding pattern will be expected when we observe chromosomes
before banding under the phase scope with the following appearance: - -
Dark.
- Which of the following factors are not important for chromosome
spreading? - -Mitotic index.
- The best method to process slides for G-banding on the same day that
they are dropped is: - -Incubate the slide in 90°C for 1-3 hrs before banding.
- You are analyzing a clinical case and discover a small extra marker with
satellites. What staining technique would be most useful in determining the
chromosomal origin of this marker? - -DAPI/DA.
- When using a fluorescein labeled probe during the FISH procedure, the
appropriate counter stain used should be: - -Propidium iodide.
- When doing sequential staining which of the following is used to remove oil
from slides? - -Xylene.
- What is the best banding technique to distinguish between 47,XY,+18 and
47,XYY? - -QFQ.
- When the identification of paired chromosomes is the primary goal, which
if the following banding techniques should be used? - -G-banding.
- When the addition of non homologous material to the q-arm of
chromosome 16 is suspected after G-banding, which if the following banding
techniques should be used? - -C-banding.
- Which chromosome is most different between C- and G-banding? - -9.
, - What is not a criteria for G-band identification? - -Location of the telomere.
- What do quinacrine and acridine orange dyes stain? - -AT rich DNA.
- What type of container should be used for collecting peripheral blood and
bone marrow specimens? - -Green top tube coated with sodium heparin.
- What type of container should be used for collecting solid tissue samples? -
-A sterile container containing media, supplemented with antibiotics.
- How do you avoid microbial contamination? - -By using aseptic technique,
sterile containers and antibiotics/fungicides.
- How do you avoid cellular contamination of prenatal specimens? - -Discard
the 1st 1-2 cc of amniotic fluid drawn.
- How do you avoid cellular contamination of solid tumor specimens? - -
Dissect away normal parenchyma and fatty tissue.
- How much specimen is required for the successful analysis of a peripheral
blood specimen? - -5-10 ml for adults and 3-5 ml for children.
- How much specimen is required for the successful analysis of a bone
marrow specimen? - -1-3 ml.
- How much specimen is required for the successful analysis of a prenatal
specimen? - -15-30 ml of amniotic fluid collected in two separate containers.
- How much specimen is required for the successful analysis of solid tissues?
- -5-25 mg of CVS, 1 mm^3 of skin biopsy and 1cm^3 of solid tumor.
- What should the label of each collection container always have? - -Patient
name and identification number.
- What does penicillin/streptomycin treat? - -Bacterial contamination.
- When do you use a kanamycin/gentamycin combination instead of other
antibiotics? - -When an organism is resistant to penicillin/streptomycin. This
combination is more broad-acting, but also a more toxic antibiotic.
- What do Amphotericin B, Fungizone and Mycostatin (Nystatin) treat? - -
Fungal contamination.
- True/False: Specimens should be shipped in direct contact with ice or dry
ice. - -False.
Questions with Answers
What are the basics of in situ hybridization? - -Pretreatment of slides with
acid, extraction of DNA with barium hydroxide, and incubation in 2xSSC to
promote denaturation.
- The following are indicators for amniocentesis except: - -A family history of
an autosomal recessive disorder.
- When a bone marrow sample cannot be delivered to the lab within 4 hrs,
the sample should: - -Be put in media and kept at room temp.
- A PB sample is clotted. What do you do? - -Break up the clot.
- All of the following information is required during the specimen set up
procedure except: - -Infectious agent.
- When should samples be logged in? - -As soon as they arrive in the
laboratory.
- How long do you wait before requesting an amniotic fluid if no cell
attachment is observed? - -10 days.
- All of the following will result in culture failure except: - -Transport at room
temp.
- Which cells would you want to use for long term serial cytogenetic studies?
- -Fibroblasts.
- The following enzymes can be used to dissociate chorionic villus samples
except for: - -RNAse.
- Which of the following is not a necessary ingredient which is added to
modified medium? - -Antibiotics.
- All of the following have buffering capability except: - -L-Glutamine.
- What type of media is necessary for the culture of Fragile X cases? - -Folic
acid deficient media.
- The most important reason why Biological Safety Caninets are used
instead of Horizontal Laminar Flow Hoods in the handling of human tissues
is: - -To protect the technologist who is processing the specimen.
, - In which of the following situations would one suspect bacterial
contamination? - -The culture media is turbid in appearance.
- Which of the following can be used for serial culture from amniotic fluid? -
-Fibroblasts.
- Which of the following chemicals is used for cryopreservation? - -Dimethyl
sulfoxide (DMSO).
- Adding the drug Colcemid to cell cultures can cause all of the following
phenomenons to occur except: - -Cell swelling.
- During slidemaking all of the following factors are important except for: - -
Slides are 1st being dipped in acetic acid.
- A good banding pattern will be expected when we observe chromosomes
before banding under the phase scope with the following appearance: - -
Dark.
- Which of the following factors are not important for chromosome
spreading? - -Mitotic index.
- The best method to process slides for G-banding on the same day that
they are dropped is: - -Incubate the slide in 90°C for 1-3 hrs before banding.
- You are analyzing a clinical case and discover a small extra marker with
satellites. What staining technique would be most useful in determining the
chromosomal origin of this marker? - -DAPI/DA.
- When using a fluorescein labeled probe during the FISH procedure, the
appropriate counter stain used should be: - -Propidium iodide.
- When doing sequential staining which of the following is used to remove oil
from slides? - -Xylene.
- What is the best banding technique to distinguish between 47,XY,+18 and
47,XYY? - -QFQ.
- When the identification of paired chromosomes is the primary goal, which
if the following banding techniques should be used? - -G-banding.
- When the addition of non homologous material to the q-arm of
chromosome 16 is suspected after G-banding, which if the following banding
techniques should be used? - -C-banding.
- Which chromosome is most different between C- and G-banding? - -9.
, - What is not a criteria for G-band identification? - -Location of the telomere.
- What do quinacrine and acridine orange dyes stain? - -AT rich DNA.
- What type of container should be used for collecting peripheral blood and
bone marrow specimens? - -Green top tube coated with sodium heparin.
- What type of container should be used for collecting solid tissue samples? -
-A sterile container containing media, supplemented with antibiotics.
- How do you avoid microbial contamination? - -By using aseptic technique,
sterile containers and antibiotics/fungicides.
- How do you avoid cellular contamination of prenatal specimens? - -Discard
the 1st 1-2 cc of amniotic fluid drawn.
- How do you avoid cellular contamination of solid tumor specimens? - -
Dissect away normal parenchyma and fatty tissue.
- How much specimen is required for the successful analysis of a peripheral
blood specimen? - -5-10 ml for adults and 3-5 ml for children.
- How much specimen is required for the successful analysis of a bone
marrow specimen? - -1-3 ml.
- How much specimen is required for the successful analysis of a prenatal
specimen? - -15-30 ml of amniotic fluid collected in two separate containers.
- How much specimen is required for the successful analysis of solid tissues?
- -5-25 mg of CVS, 1 mm^3 of skin biopsy and 1cm^3 of solid tumor.
- What should the label of each collection container always have? - -Patient
name and identification number.
- What does penicillin/streptomycin treat? - -Bacterial contamination.
- When do you use a kanamycin/gentamycin combination instead of other
antibiotics? - -When an organism is resistant to penicillin/streptomycin. This
combination is more broad-acting, but also a more toxic antibiotic.
- What do Amphotericin B, Fungizone and Mycostatin (Nystatin) treat? - -
Fungal contamination.
- True/False: Specimens should be shipped in direct contact with ice or dry
ice. - -False.
