Microbiology lab practical exam 1 Questions and Answers 100% Pass

Microbiology lab practical exam 1 Questions and Answers 100% Pass Wet mount steps: - 2. specimen 3. Cover slip over specimen hanging drop steps: - 1. Vaseline around cover slip 2. micro organism in center of cover slip 3. place concave slide on cover slip Gram stain steps: - 1. Crystal Violet 2. Gram's Iodine 3. Ethyl Alcohol 4. Safranin Red negative stain steps: - 1. nigrosin loop ria 4. slide to spread mixture 5. air dry acid-fast stain steps: - 1. carbolfuchsin 2 | P a g e Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE 2. acid alcohol 3. methylene blue 4. wash & dry simple stain steps - 1. fixed smear slide 2. dyes 3. rinse & dry nutrient broth - -liquid media used to grow bacteria agar - -used to culture microorganisms candle jar - -used for anaerobic organisms Petri dish - -Used to culture or observe bacteria growth slant - -a medium for which bacteria can grow on. E.coli Gram stain - red colored rods S. epidermidis Gram stain - purple colored grapes B. subtilis Gram stain - purple colored rods list information gained from a particular slide based on staining techniques (cell morphology, arrangement, cell wall structure, Gram stain reaction or other staining reaction). - ... show motility on a hanging drop - movement because of flagella show motility on a Brownian movement. - bacteria moves randomly, due to the energy of a system (vibration) 3 | P a g e Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE why does motility occur in bacteria ? - they have a flagellum that propels them around. why does motility occur in protozoans? - 1. Flagella 2. Cilia 3. Amoeba aseptic technique steps - 1. flame loop 2. flame tube 3. inoculate bacteria 4. flame loop 5. flame tube solid culture set up - 1- water