Microbiology Lab – Stains Exam Questions and
Answers 100% Pass
Simple stain - ✔✔-Add color to cells for viewing
-Highlight entire organism's shape and basic structures
-Includes:
Methylene blue
Safranin
Crystal violet
Carbolfuchsin;Negative stain - ✔✔-Enhances viewing of morphology, capsules
-Bacteria are live
-No heat fixing! (no denaturing)
-Nigrosin ink
-View under oil
-Anionic (acidic) simple stain;Gram stain - ✔✔-Determines cell wall type (gram positive or negative)
-Differential stain;Gram stain procedure - ✔✔1. Crystal violet
2. Iodine (mordant)
3. Alcohol (decolorizer)
4. Safranin (counterstain)
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