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Microbiology Lab Practical – Final Exam
Questions and Answers 100% Pass
Staph epidermidis - ✔✔This is MSA and the side labelled "2" is red. Which Staph does this indicate?
Staph aureus - ✔✔This is MSA and the side labelled "1" has turned yellow. Which Staph does this
indicate?
Lactose Negative - ✔✔This is an EMB plate. What does the test result of "1" indicate?
Lactose Positive - ✔✔This is an EMB plate. What does the test result of "2" indicate?
E. Coli - ✔✔This is an EMB plate. What is indicated by "3?"
Starch was broken down - ✔✔This is a starch plate. What does the clear boundary around this colony
indicate?
acid was produced - ✔✔Why did this Fermentation Tube turn yellow?
gas was produced - ✔✔What is indicated by the bubble in this Durham Tube inside a Fermentation Tube?
Protein was not broken down - ✔✔This is a Gelatin Tube Test. In Test Tube 2, it is solid. What does this
mean?
Catalase Test - ✔✔What is the name of this test?
Antibiotic Disk Diffusion (Antibiotic Susceptibility) - ✔✔What is the name of this test?
Mannitol Salt Agar - ✔✔What is the name of this media?
Urea Broth
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,2|Page
Urease Enzyme
Tube 1: Positive
Tube 2: Negative - ✔✔What is it testing for?
What does pink indicate?
What does the pale yellow indicate?
Nitrate Reduction - ✔✔What is the name of this test?
neutrophil - ✔✔
lymphocyte - ✔✔
monocyte - ✔✔
eosinophil - ✔✔
basophil - ✔✔
What method is used for the antimicrobial susceptibility test? - ✔✔Kirby-Bauer
What technique is used for the Kirby-Bauer test? - ✔✔swab and make a bacterial lawn
What is the principle of technique for the Kirby-Bauer? - ✔✔disk diffusion
What observations are made in lab with Kirby-Bauer? - ✔✔zone of inhibition
What is reported out to Physicians from the Kirby-Bauer? - ✔✔susceptibility or resistance
What is produced when UV radiation is absorbs by DNA? - ✔✔pyrimidine dimers - which interferes with
DNA replication and transcription
Which bacteria has mechanisms to repair damage from UV radiation? - ✔✔Escherichia coli
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
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What is a major limitation to UV radiation? - ✔✔It cannot pass through solid surfaces
Why did we use an index card or leave the lid on during the experiment? - ✔✔To act as a control - we
wanted to see growth of bacteria without UV light interaction
What technique was used for UV test? - ✔✔swab and make a bacterial lawn
What kind of radiation is UV? - ✔✔non-ionizing radiation
What type of agar is used for Bacterial Transformation lab? - ✔✔LB - Luria Bertani
What is transformation? - ✔✔When bacteria can pick up DNA from the environment and make use of the
genes it carries
What is GFP? - ✔✔green fluorescent protein - responsible for bioluminescence in jellyfish
Why are we using GFP? - ✔✔to show visual evidence that the DNA transformed with successful gene
expression
What is ARA? - ✔✔arabinose (promoter that activates GFP and Amp resistance genes)
What is the vector used in the transformation lab? - ✔✔pGLO plasmid
What does the amp resistance gene release? - ✔✔Beta lactamase
What are we doing to the E.coli bacteria in the transformation experiment? - ✔✔We are making the
bacteria competent by making the (-) bacteria accept DNA which is (-)
What two items did we use in lab to make E.coli competent? - ✔✔-transforming solution (CaCl2)
-heat shock
What will negate the charge of DNA in the transformation lab? - ✔✔transforming solution
What will allow the DNA (plasmid) to enter the E.coli? - ✔✔heat shock (opens pores)
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
Microbiology Lab Practical – Final Exam
Questions and Answers 100% Pass
Staph epidermidis - ✔✔This is MSA and the side labelled "2" is red. Which Staph does this indicate?
Staph aureus - ✔✔This is MSA and the side labelled "1" has turned yellow. Which Staph does this
indicate?
Lactose Negative - ✔✔This is an EMB plate. What does the test result of "1" indicate?
Lactose Positive - ✔✔This is an EMB plate. What does the test result of "2" indicate?
E. Coli - ✔✔This is an EMB plate. What is indicated by "3?"
Starch was broken down - ✔✔This is a starch plate. What does the clear boundary around this colony
indicate?
acid was produced - ✔✔Why did this Fermentation Tube turn yellow?
gas was produced - ✔✔What is indicated by the bubble in this Durham Tube inside a Fermentation Tube?
Protein was not broken down - ✔✔This is a Gelatin Tube Test. In Test Tube 2, it is solid. What does this
mean?
Catalase Test - ✔✔What is the name of this test?
Antibiotic Disk Diffusion (Antibiotic Susceptibility) - ✔✔What is the name of this test?
Mannitol Salt Agar - ✔✔What is the name of this media?
Urea Broth
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
,2|Page
Urease Enzyme
Tube 1: Positive
Tube 2: Negative - ✔✔What is it testing for?
What does pink indicate?
What does the pale yellow indicate?
Nitrate Reduction - ✔✔What is the name of this test?
neutrophil - ✔✔
lymphocyte - ✔✔
monocyte - ✔✔
eosinophil - ✔✔
basophil - ✔✔
What method is used for the antimicrobial susceptibility test? - ✔✔Kirby-Bauer
What technique is used for the Kirby-Bauer test? - ✔✔swab and make a bacterial lawn
What is the principle of technique for the Kirby-Bauer? - ✔✔disk diffusion
What observations are made in lab with Kirby-Bauer? - ✔✔zone of inhibition
What is reported out to Physicians from the Kirby-Bauer? - ✔✔susceptibility or resistance
What is produced when UV radiation is absorbs by DNA? - ✔✔pyrimidine dimers - which interferes with
DNA replication and transcription
Which bacteria has mechanisms to repair damage from UV radiation? - ✔✔Escherichia coli
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
, 3|Page
What is a major limitation to UV radiation? - ✔✔It cannot pass through solid surfaces
Why did we use an index card or leave the lid on during the experiment? - ✔✔To act as a control - we
wanted to see growth of bacteria without UV light interaction
What technique was used for UV test? - ✔✔swab and make a bacterial lawn
What kind of radiation is UV? - ✔✔non-ionizing radiation
What type of agar is used for Bacterial Transformation lab? - ✔✔LB - Luria Bertani
What is transformation? - ✔✔When bacteria can pick up DNA from the environment and make use of the
genes it carries
What is GFP? - ✔✔green fluorescent protein - responsible for bioluminescence in jellyfish
Why are we using GFP? - ✔✔to show visual evidence that the DNA transformed with successful gene
expression
What is ARA? - ✔✔arabinose (promoter that activates GFP and Amp resistance genes)
What is the vector used in the transformation lab? - ✔✔pGLO plasmid
What does the amp resistance gene release? - ✔✔Beta lactamase
What are we doing to the E.coli bacteria in the transformation experiment? - ✔✔We are making the
bacteria competent by making the (-) bacteria accept DNA which is (-)
What two items did we use in lab to make E.coli competent? - ✔✔-transforming solution (CaCl2)
-heat shock
What will negate the charge of DNA in the transformation lab? - ✔✔transforming solution
What will allow the DNA (plasmid) to enter the E.coli? - ✔✔heat shock (opens pores)
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
