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Microbiology Lab Final Exam Questions and
Answers 100% Pass
All UTA microbiology labs are considered what biosafety level? - ✔✔BSL-2
What are four things that all BSL-2 labs must include? - ✔✔1. PPE
2. Sinks and eye wash stations
3. Restricted access
4. autoclaves (or alternative decontamination methods)
What is the plural for media? - ✔✔medium
media can come in a wide variety of formulations and can exist in what three states? - ✔✔solid, semi-
solid, and liquid
Catalase is found in both - ✔✔prokaryotes and eukaryotes
What is often considered as a virulence factor for many pathogens? - ✔✔coagulase
What large amino acid polymer is found in the vertebrate connective tissues? - ✔✔gelatin
What large amino acid polymer is found in mammalian milk? - ✔✔Casein
A phenol red test has a pH indicator that is what color? - ✔✔red
In gelatin hydrolysis tests media contains what besides gelatin? - ✔✔peptone and beef extract nutrients
Urea is a nitrogen containing organic molecule and principal end product of - ✔✔protein catabolism
Durham tube in phenol red test is used to - ✔✔capture gas produced by the microbe
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
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Amylase enzymes are able to cleave starch molecules by breaking what? - ✔✔glycosidic bonds between
glucose monomers
What is the term for water molecules breaking complex molecules into simpler forms? - ✔✔hydrolysis
E. coli and S. faecalis are considered good sewage indicators because they are not normally - ✔✔present
in water or soil
Are coliforms gram-negative or gram-positive coccus? - ✔✔gram-negative
Microorganisms capable of producing citrate permease will be able to utilize citrate as their - ✔✔sole
carbon source
What is the enzyme that some microorganisms produce that help it grow on Simmons citrate medium? -
✔✔citrate permease
How do you know if a microorganism is is gelatinase positive? - ✔✔Liquify gelatin
Kovac's reagent reacts with what to produce a red color in SIM test? - ✔✔indole
What percentage of evaporated milk is added to tryptic soy agar for the casein hydrolysis test? - ✔✔3%
What type of media is Mitis salivarious agar? - ✔✔both selective and differential
Bile Esculin agar media is selective because what compound inhibits growth of gram negative bacilli and
most gram positives? - ✔✔sodium azide
If a microorganism is coagulase positive, clotting of plasma cells can be observed - ✔✔within one minute
The MR-VP test is performed to determine a microorganism's ability to - ✔✔ferment glucose and produce
by-products
Are anaerobic microbes capable of making catalase? - ✔✔no
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
Microbiology Lab Final Exam Questions and
Answers 100% Pass
All UTA microbiology labs are considered what biosafety level? - ✔✔BSL-2
What are four things that all BSL-2 labs must include? - ✔✔1. PPE
2. Sinks and eye wash stations
3. Restricted access
4. autoclaves (or alternative decontamination methods)
What is the plural for media? - ✔✔medium
media can come in a wide variety of formulations and can exist in what three states? - ✔✔solid, semi-
solid, and liquid
Catalase is found in both - ✔✔prokaryotes and eukaryotes
What is often considered as a virulence factor for many pathogens? - ✔✔coagulase
What large amino acid polymer is found in the vertebrate connective tissues? - ✔✔gelatin
What large amino acid polymer is found in mammalian milk? - ✔✔Casein
A phenol red test has a pH indicator that is what color? - ✔✔red
In gelatin hydrolysis tests media contains what besides gelatin? - ✔✔peptone and beef extract nutrients
Urea is a nitrogen containing organic molecule and principal end product of - ✔✔protein catabolism
Durham tube in phenol red test is used to - ✔✔capture gas produced by the microbe
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
, 2|Page
Amylase enzymes are able to cleave starch molecules by breaking what? - ✔✔glycosidic bonds between
glucose monomers
What is the term for water molecules breaking complex molecules into simpler forms? - ✔✔hydrolysis
E. coli and S. faecalis are considered good sewage indicators because they are not normally - ✔✔present
in water or soil
Are coliforms gram-negative or gram-positive coccus? - ✔✔gram-negative
Microorganisms capable of producing citrate permease will be able to utilize citrate as their - ✔✔sole
carbon source
What is the enzyme that some microorganisms produce that help it grow on Simmons citrate medium? -
✔✔citrate permease
How do you know if a microorganism is is gelatinase positive? - ✔✔Liquify gelatin
Kovac's reagent reacts with what to produce a red color in SIM test? - ✔✔indole
What percentage of evaporated milk is added to tryptic soy agar for the casein hydrolysis test? - ✔✔3%
What type of media is Mitis salivarious agar? - ✔✔both selective and differential
Bile Esculin agar media is selective because what compound inhibits growth of gram negative bacilli and
most gram positives? - ✔✔sodium azide
If a microorganism is coagulase positive, clotting of plasma cells can be observed - ✔✔within one minute
The MR-VP test is performed to determine a microorganism's ability to - ✔✔ferment glucose and produce
by-products
Are anaerobic microbes capable of making catalase? - ✔✔no
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
