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Chapter 3 - Microbiology Lab Exam Questions and
Answers 100% Pass
Name the parts of the Compound Light Microscope: Eye piece, Objective, Stage, Light Control, Base,
Focus - ✔✔
Dark-field Microscope - ✔✔used when the microbes are not visible with the use of the light microscope;
when microbes cannot be stained;
when microbes would be distorted by staining
Phase-Contrast Microscope - ✔✔allows detailed visualization of the internal structures in living microbes
Fluorescence Microscope - ✔✔Immunofluorescence;
detecting bacteria and other pathogens within cells and tissues;
Dx of rabies and Syphilis
Electron Microscope - ✔✔ability to visualize viruses, the internal structure of cells, and other objects
smaller than 0.2 um;
uses a beam of electrons
Transmission electron microscope/TEM - ✔✔utilizes a focused beam of electrons that are emitted from
an electron gun and pass thru a very thin section of the specimen
Scanning electron microscope/SEM - ✔✔section of specimen does NOT have to be thinly sliced like with
the TEM;
3D views are obtained
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
Chapter 3 - Microbiology Lab Exam Questions and
Answers 100% Pass
Name the parts of the Compound Light Microscope: Eye piece, Objective, Stage, Light Control, Base,
Focus - ✔✔
Dark-field Microscope - ✔✔used when the microbes are not visible with the use of the light microscope;
when microbes cannot be stained;
when microbes would be distorted by staining
Phase-Contrast Microscope - ✔✔allows detailed visualization of the internal structures in living microbes
Fluorescence Microscope - ✔✔Immunofluorescence;
detecting bacteria and other pathogens within cells and tissues;
Dx of rabies and Syphilis
Electron Microscope - ✔✔ability to visualize viruses, the internal structure of cells, and other objects
smaller than 0.2 um;
uses a beam of electrons
Transmission electron microscope/TEM - ✔✔utilizes a focused beam of electrons that are emitted from
an electron gun and pass thru a very thin section of the specimen
Scanning electron microscope/SEM - ✔✔section of specimen does NOT have to be thinly sliced like with
the TEM;
3D views are obtained
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
