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Micro Lab Exam 2 transduction questions and Answers 100% Pass

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Micro Lab Exam 2 transduction questions and Answers 100% Pass Why do we do plate-streaking? - to create countable colonies, and identify them What are the two materials used in an Ab susceptibility test, and how do you inoculate the plate? - alcohol wipes, and forceps; bacterial lawn What two things does the zone of inhibition depend on? - diffusion of the molecule, and susceptibility What are the effects the zone of inhibition has on bacteria? - bactericidal or bacteriostatic What kind of test are enterotubes considered? - biochemical tests/enzymes/color changes What does a color change in an enterotube signify? - enzyme broke down whatever was in the medium What was a technique used in the enterotubes? - aerobic holes What do enterotubes identify? - enteric gram negative bacilli In a UV light test, specific damage is done to the bacteria with or without the lid? - without How is bacteria damaged in UV light tests? - damages DNA via thymine diamers Which bacteria seemed to repair the UV damage better, and why? - B. subtilis; E coli isn't exposed to UV because its always in the intestine, whereas B subtilis lives in the soil, so its adapted When did you see the most damage? - 15-20 minutes What makes electrophoresis sink? - sucrose

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Microbiology Lab
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Microbiology Lab

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Micro Lab Exam 2 transduction questions and
Answers 100% Pass


Why do we do plate-streaking? - ✔✔to create countable colonies, and identify them


What are the two materials used in an Ab susceptibility test, and how do you inoculate the plate? -

✔✔alcohol wipes, and forceps; bacterial lawn


What two things does the zone of inhibition depend on? - ✔✔diffusion of the molecule, and susceptibility


What are the effects the zone of inhibition has on bacteria? - ✔✔bactericidal or bacteriostatic


What kind of test are enterotubes considered? - ✔✔biochemical tests/enzymes/color changes


What does a color change in an enterotube signify? - ✔✔enzyme broke down whatever was in the

medium


What was a technique used in the enterotubes? - ✔✔aerobic holes


What do enterotubes identify? - ✔✔enteric gram negative bacilli


In a UV light test, specific damage is done to the bacteria with or without the lid? - ✔✔without


How is bacteria damaged in UV light tests? - ✔✔damages DNA via thymine diamers


Which bacteria seemed to repair the UV damage better, and why? - ✔✔B. subtilis; E coli isn't exposed to

UV because its always in the intestine, whereas B subtilis lives in the soil, so its adapted


When did you see the most damage? - ✔✔15-20 minutes


What makes electrophoresis sink? - ✔✔sucrose




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