Micro Lab Exam 2 transduction questions and
Answers 100% Pass
Why do we do plate-streaking? - ✔✔to create countable colonies, and identify them
What are the two materials used in an Ab susceptibility test, and how do you inoculate the plate? -
✔✔alcohol wipes, and forceps; bacterial lawn
What two things does the zone of inhibition depend on? - ✔✔diffusion of the molecule, and susceptibility
What are the effects the zone of inhibition has on bacteria? - ✔✔bactericidal or bacteriostatic
What kind of test are enterotubes considered? - ✔✔biochemical tests/enzymes/color changes
What does a color change in an enterotube signify? - ✔✔enzyme broke down whatever was in the
medium
What was a technique used in the enterotubes? - ✔✔aerobic holes
What do enterotubes identify? - ✔✔enteric gram negative bacilli
In a UV light test, specific damage is done to the bacteria with or without the lid? - ✔✔without
How is bacteria damaged in UV light tests? - ✔✔damages DNA via thymine diamers
Which bacteria seemed to repair the UV damage better, and why? - ✔✔B. subtilis; E coli isn't exposed to
UV because its always in the intestine, whereas B subtilis lives in the soil, so its adapted
When did you see the most damage? - ✔✔15-20 minutes
What makes electrophoresis sink? - ✔✔sucrose
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