GMS 6552 Exam 2 questions with 100%
correct answers
MODULE 5 - correct answer ✔✔
An oncogene is defined as - correct answer ✔✔a gene that causes or contributes to the development of
cancer
What are the two types of evidence for oncogenic behavior? - correct answer ✔✔In vivo
In vitro
What are the 4 criteria for In vivo oncogenic behavior - correct answer ✔✔Cause cancer when activated
in a transgenic animal
Render nontumorigenic cells tumorigenic
Knock down or knock out in tumorigenic cells renders the cells nontumorigenic
Activation/overexpression of the oncogene is strongly correlated with human cancer
What are the 3 criteria for In vitro oncogenic behavior - correct answer ✔✔Causes anchorage-
independent growth in soft agar (protection from anoikis)
Causes focus formation (loss of contact inhibition)
Renders cells growth factor independent
What is an example of a GTPase that plays a biochemical role in oncogenes. - correct answer ✔✔Ras
What is an example of a Protein Kinases that plays a biochemical role in oncogenes. - correct answer
✔✔Raf, Akt, BCR-ABL, Cdk4, Src
What is an example of a Lipid Kinases that plays a biochemical role in oncogenes. - correct answer
✔✔PI3-Kinase
, What is an example of a Transcription factors that plays a biochemical role in oncogenes. - correct
answer ✔✔Myc, Fos, Jun, E2F1, ....
What is an example of a Binding and inactivating apoptotic proteins that plays a biochemical role in
oncogenes. - correct answer ✔✔BCL2
What is an example of a Protein Kinase Regulatory Subunits that plays a biochemical role in oncogenes. -
correct answer ✔✔Cyclins A, E, and D
What is an example of a Transcriptional coactivators that plays a biochemical role in oncogenes. - correct
answer ✔✔ß-Catenin, YAP, TAZ
Five Ways to Activate Oncogenes - correct answer ✔✔1. Mutation of genes to make it overactive.
2. Amplification of a normal gene
3. Chromosomal Rearrangement
4. Promoter/enhancer insertion
5. Hypomethylation of oncogene
What are some examples of Chromosome Rearrangement? (2) - correct answer ✔✔Philadelphia
chromosome. balanced reciprocal translocation t(9:22) in myeloid cells. Fuses 5' end of BCR gene (chr.22)
to the coding region of oncogene ABL
Burkitt's lymphoma t(8;14) juxtaposes Ig heavy chain locus promoter/enhancer region and MYC
oncogene. Results in high levels of MYC transcription in B cells. The protein is normal but overexpressed.
What therapy choices do we have to treat HER2 breast cancer? - correct answer ✔✔Herceptin,
Pertuzumab, and Lapatinib
If the philedelphia chromosome is present and we have a lessened amount of BCR-ABL tyr kinases, how
do we treat it? - correct answer ✔✔Gleevec
correct answers
MODULE 5 - correct answer ✔✔
An oncogene is defined as - correct answer ✔✔a gene that causes or contributes to the development of
cancer
What are the two types of evidence for oncogenic behavior? - correct answer ✔✔In vivo
In vitro
What are the 4 criteria for In vivo oncogenic behavior - correct answer ✔✔Cause cancer when activated
in a transgenic animal
Render nontumorigenic cells tumorigenic
Knock down or knock out in tumorigenic cells renders the cells nontumorigenic
Activation/overexpression of the oncogene is strongly correlated with human cancer
What are the 3 criteria for In vitro oncogenic behavior - correct answer ✔✔Causes anchorage-
independent growth in soft agar (protection from anoikis)
Causes focus formation (loss of contact inhibition)
Renders cells growth factor independent
What is an example of a GTPase that plays a biochemical role in oncogenes. - correct answer ✔✔Ras
What is an example of a Protein Kinases that plays a biochemical role in oncogenes. - correct answer
✔✔Raf, Akt, BCR-ABL, Cdk4, Src
What is an example of a Lipid Kinases that plays a biochemical role in oncogenes. - correct answer
✔✔PI3-Kinase
, What is an example of a Transcription factors that plays a biochemical role in oncogenes. - correct
answer ✔✔Myc, Fos, Jun, E2F1, ....
What is an example of a Binding and inactivating apoptotic proteins that plays a biochemical role in
oncogenes. - correct answer ✔✔BCL2
What is an example of a Protein Kinase Regulatory Subunits that plays a biochemical role in oncogenes. -
correct answer ✔✔Cyclins A, E, and D
What is an example of a Transcriptional coactivators that plays a biochemical role in oncogenes. - correct
answer ✔✔ß-Catenin, YAP, TAZ
Five Ways to Activate Oncogenes - correct answer ✔✔1. Mutation of genes to make it overactive.
2. Amplification of a normal gene
3. Chromosomal Rearrangement
4. Promoter/enhancer insertion
5. Hypomethylation of oncogene
What are some examples of Chromosome Rearrangement? (2) - correct answer ✔✔Philadelphia
chromosome. balanced reciprocal translocation t(9:22) in myeloid cells. Fuses 5' end of BCR gene (chr.22)
to the coding region of oncogene ABL
Burkitt's lymphoma t(8;14) juxtaposes Ig heavy chain locus promoter/enhancer region and MYC
oncogene. Results in high levels of MYC transcription in B cells. The protein is normal but overexpressed.
What therapy choices do we have to treat HER2 breast cancer? - correct answer ✔✔Herceptin,
Pertuzumab, and Lapatinib
If the philedelphia chromosome is present and we have a lessened amount of BCR-ABL tyr kinases, how
do we treat it? - correct answer ✔✔Gleevec
