Exam 1 GMS 7133 questions well
answered already passed
What are the basics of coronavirus?
(Enveloped/Naked), ______-sense RNA viruses
The _____ genome of all RNA viruses
Unique replication strategy results in a high frequency of _____
Mature virions bud at _____ membranes
Infect many species including _____, causing a range of diseases
Since the occurrence of SARS, have become considered as '_____pathogens' - correct answer
✔✔Enveloped, positive-sense RNA viruses
The largest genome of all RNA viruses
Unique replication strategy results in a high frequency of recombination
Mature virions bud at intracellular membranes
Infect many species including humans, causing a range of diseases
Since the occurrence of SARS, have become considered as 'emerging pathogens'
The Nidoviralesorder of ____-sense RNA viruses make nested sets of mRNAs - correct answer
✔✔positive
What class is Covid-19 a part of?
A) Alpha-CoV
B) Beta-CoV
C)Gamma-CoV
D) Delta-CoV - correct answer ✔✔B)Beta-CoV
,Human coronaviruses cause ____ disease of varyings severity - correct answer ✔✔respiratory
What is the order and family of coronavirus? - correct answer ✔✔Order:
Nidovirales
Family:
Coronaviridae
Describe the basic structure of coronavirus?
N:
M:
S:
HE:
E: - correct answer ✔✔N: Nucleocapsid
M: Membrane glycoprotein
S: Spike glycoproteins
HE: Hemagglutinin-esterase glycoprotein (not all CoVs have this spike)
E: Small envelope glycoprotein
,Describe the envelope - correct answer ✔✔~80-120nm
Petal-shaped spikes = S protein, give virions crown-like morphology
Fringe of shorter spikes = HE
What shape is the nucleocapsid - correct answer ✔✔Helical
Where does budding occur? - correct answer ✔✔into intracellular vesicles
What is the function of the S glycoprotein? - correct answer ✔✔promotes receptor binding and fusion
20nm spikes HEAVILY glycosylated
In BCoV & MHV: S cleaved to S1, S2 - remain associated
T/F: In some CoV, S protein fusion activity requires proteolytic cleavage at one site - correct answer
✔✔False
In some CoV, S protein fusion activity requires proteolytic cleavage at one site or two sites
How many structural domains does an S Glycoprotein have? Name them and their functions. - correct
answer ✔✔4 domains from N to C-terminal:
S1 = globular head
S2 = more conserved sequence, fusion-promoting, external stalk
Transmembrane domain
Cytoplasmic domain
What is the function of HE protein? - correct answer ✔✔smaller spikes 5-7nm
found on some group I and II coronaviruses, NOT SARS
, frequently deleted during cell passaging = non essential
65-70kD glycosylated disulfide-linked dimer
Hemagglutination activity = binds sialic acid = involved in binding?
Esterase activity = degrades sialic acid = involved in release of virus from cells?
What is the function of M protein?
Short N-terminal domain _____of envelope, spans membrane 3 times and large C-terminal domain
INSIDE envelope
the primary component of internal ____-shell
viral membrane unusually ____, probably because the C-terminal region of M forms an extra internal
layer
MOST _____ envelope protein
_____for budding
Required for budding of virions; targeted to ____membranes - correct answer ✔✔Short N-terminal
domain OUTSIDE of envelope, spans membrane 3 times and large C-terminal domain INSIDE envelope
the primary component of internal core-shell
viral membrane unusually thick, probably because the C-terminal region of M forms an extra internal
layer
answered already passed
What are the basics of coronavirus?
(Enveloped/Naked), ______-sense RNA viruses
The _____ genome of all RNA viruses
Unique replication strategy results in a high frequency of _____
Mature virions bud at _____ membranes
Infect many species including _____, causing a range of diseases
Since the occurrence of SARS, have become considered as '_____pathogens' - correct answer
✔✔Enveloped, positive-sense RNA viruses
The largest genome of all RNA viruses
Unique replication strategy results in a high frequency of recombination
Mature virions bud at intracellular membranes
Infect many species including humans, causing a range of diseases
Since the occurrence of SARS, have become considered as 'emerging pathogens'
The Nidoviralesorder of ____-sense RNA viruses make nested sets of mRNAs - correct answer
✔✔positive
What class is Covid-19 a part of?
A) Alpha-CoV
B) Beta-CoV
C)Gamma-CoV
D) Delta-CoV - correct answer ✔✔B)Beta-CoV
,Human coronaviruses cause ____ disease of varyings severity - correct answer ✔✔respiratory
What is the order and family of coronavirus? - correct answer ✔✔Order:
Nidovirales
Family:
Coronaviridae
Describe the basic structure of coronavirus?
N:
M:
S:
HE:
E: - correct answer ✔✔N: Nucleocapsid
M: Membrane glycoprotein
S: Spike glycoproteins
HE: Hemagglutinin-esterase glycoprotein (not all CoVs have this spike)
E: Small envelope glycoprotein
,Describe the envelope - correct answer ✔✔~80-120nm
Petal-shaped spikes = S protein, give virions crown-like morphology
Fringe of shorter spikes = HE
What shape is the nucleocapsid - correct answer ✔✔Helical
Where does budding occur? - correct answer ✔✔into intracellular vesicles
What is the function of the S glycoprotein? - correct answer ✔✔promotes receptor binding and fusion
20nm spikes HEAVILY glycosylated
In BCoV & MHV: S cleaved to S1, S2 - remain associated
T/F: In some CoV, S protein fusion activity requires proteolytic cleavage at one site - correct answer
✔✔False
In some CoV, S protein fusion activity requires proteolytic cleavage at one site or two sites
How many structural domains does an S Glycoprotein have? Name them and their functions. - correct
answer ✔✔4 domains from N to C-terminal:
S1 = globular head
S2 = more conserved sequence, fusion-promoting, external stalk
Transmembrane domain
Cytoplasmic domain
What is the function of HE protein? - correct answer ✔✔smaller spikes 5-7nm
found on some group I and II coronaviruses, NOT SARS
, frequently deleted during cell passaging = non essential
65-70kD glycosylated disulfide-linked dimer
Hemagglutination activity = binds sialic acid = involved in binding?
Esterase activity = degrades sialic acid = involved in release of virus from cells?
What is the function of M protein?
Short N-terminal domain _____of envelope, spans membrane 3 times and large C-terminal domain
INSIDE envelope
the primary component of internal ____-shell
viral membrane unusually ____, probably because the C-terminal region of M forms an extra internal
layer
MOST _____ envelope protein
_____for budding
Required for budding of virions; targeted to ____membranes - correct answer ✔✔Short N-terminal
domain OUTSIDE of envelope, spans membrane 3 times and large C-terminal domain INSIDE envelope
the primary component of internal core-shell
viral membrane unusually thick, probably because the C-terminal region of M forms an extra internal
layer
