UNLV BIO 351 LAB FINAL PRACTICAL STUDY
EXAM
Gram-positive - ANSWER Thick peptidoglycan (20-80 nm)
Gram-negative - ANSWER Thin peptidoglycan (2-7 nm). The outer membrane
of lipopolysaccharides
Complex/undefined - ANSWER The exact composition and amounts of
carbon and nitrogen in media are unknown. Ex TSA or TSB
Defined/synthetic - ANSWER The exact composition and quantities of all
ingredients of media are known. Ex. Simmons Citrate Agar
All-purpose media - ANSWER Designed to support growth of a wide range of
organisms. Ex. TSA
Selective - ANSWER Inhibit growth of certain micro organisms and allows
growth of others. Ex. Mannitol Salt Agar
Differential - ANSWER Uses indicator such as pH. Ex. ManConkey Agar
Autoclaving - ANSWER High pressure and temperature to sterilize.
Conditions are 121C and 15psi for 15-20 minutes
Dry-Heat Sterilization - ANSWER Sterilization of glass wear. 160-170C for 2
hours.
Ultra Violet Radiation - ANSWER Surface sterilization
Chemical Sterilization - ANSWER Used for heat sensitive materials. Ex.
Ethylene oxide
Streak Plate Method of Isolation - ANSWER Produces a dilution gradient
which allows growth of colony forming units (CFU)
, Colony - ANSWER A visible mass of cells, all of which are genetically
identical
Limit of Resolution - ANSWER Te actual measurement of how far apart two
points must be for the microscope to view them as separate. 0.2 um
Numerical Aperature - ANSWER Measure of a ens's ability to capture light
coming from the specimen and use it to make the image.
Parfocal lens System - ANSWER System that allows an object to stay in focus
when the objective lens is changed
Nostoc Demo - ANSWER Cyanobacteria containing heterocyst for nitrogen
fixatoin
Basic Stain - ANSWER Positively charged and therefore adhere to the
bacterial membrane which is negatively charged. Ex. crystal violet,
carbolfuchsin, safranin, and methylene blue.
Acid Stain - ANSWER Negatively charged and will be repelled by bacterial
membrane, thus only the background will be stained. Ex. nigrosin.
Alternate to Gram Stain - ANSWER 3%KOH
Endospore - ANSWER a dormant and highly resistant form of a bacterium that
can survive harsh and stressful conditions. Contain keratin.
Vegetative Cell - ANSWER A cell lacking an endospore
Sporangium - ANSWER The part of the cell surrounding the mature
endospore
Free Spores - ANSWER Cell lyses to release the endospore
Nutrient Sporulations Medium (NSM) - ANSWER Contains Ca2+ Mn2+and
Mg2+. Induce sporulation after 5 days of being in medium
EXAM
Gram-positive - ANSWER Thick peptidoglycan (20-80 nm)
Gram-negative - ANSWER Thin peptidoglycan (2-7 nm). The outer membrane
of lipopolysaccharides
Complex/undefined - ANSWER The exact composition and amounts of
carbon and nitrogen in media are unknown. Ex TSA or TSB
Defined/synthetic - ANSWER The exact composition and quantities of all
ingredients of media are known. Ex. Simmons Citrate Agar
All-purpose media - ANSWER Designed to support growth of a wide range of
organisms. Ex. TSA
Selective - ANSWER Inhibit growth of certain micro organisms and allows
growth of others. Ex. Mannitol Salt Agar
Differential - ANSWER Uses indicator such as pH. Ex. ManConkey Agar
Autoclaving - ANSWER High pressure and temperature to sterilize.
Conditions are 121C and 15psi for 15-20 minutes
Dry-Heat Sterilization - ANSWER Sterilization of glass wear. 160-170C for 2
hours.
Ultra Violet Radiation - ANSWER Surface sterilization
Chemical Sterilization - ANSWER Used for heat sensitive materials. Ex.
Ethylene oxide
Streak Plate Method of Isolation - ANSWER Produces a dilution gradient
which allows growth of colony forming units (CFU)
, Colony - ANSWER A visible mass of cells, all of which are genetically
identical
Limit of Resolution - ANSWER Te actual measurement of how far apart two
points must be for the microscope to view them as separate. 0.2 um
Numerical Aperature - ANSWER Measure of a ens's ability to capture light
coming from the specimen and use it to make the image.
Parfocal lens System - ANSWER System that allows an object to stay in focus
when the objective lens is changed
Nostoc Demo - ANSWER Cyanobacteria containing heterocyst for nitrogen
fixatoin
Basic Stain - ANSWER Positively charged and therefore adhere to the
bacterial membrane which is negatively charged. Ex. crystal violet,
carbolfuchsin, safranin, and methylene blue.
Acid Stain - ANSWER Negatively charged and will be repelled by bacterial
membrane, thus only the background will be stained. Ex. nigrosin.
Alternate to Gram Stain - ANSWER 3%KOH
Endospore - ANSWER a dormant and highly resistant form of a bacterium that
can survive harsh and stressful conditions. Contain keratin.
Vegetative Cell - ANSWER A cell lacking an endospore
Sporangium - ANSWER The part of the cell surrounding the mature
endospore
Free Spores - ANSWER Cell lyses to release the endospore
Nutrient Sporulations Medium (NSM) - ANSWER Contains Ca2+ Mn2+and
Mg2+. Induce sporulation after 5 days of being in medium
