BIO 351 EXAM 1 QUESTIONS AND VERIFIED
ANSWERS
The following is a diagram from Ensembl of the MC1R gene that regulates red
hair color. Where is the translational start site?
A is the transcriptional start site
Diagram of a protein coding gene
The following is a diagram from Ensembl of the MC1R gene that regulates red
hair color. Which of the following represents the 5' UTR?
Diagram of a protein coding gene
(untranslated regions)
What is the genomic signal that tells the cell to start transcription?
the promoter sequences are required to initiate transcription
, Enhancers
they are the sites of transcription factor binding.
Have Flexibility in Their Orientation and Localization
Tissue Specific and Modular
In individuals with the Duffy mutation that increases resistance to malaria, what
level of expression to you expect for the Duffy protein in the endothelial and
Purkinje (brain) cells?
normal; enhancers are tissue-specific. That means that the effects on the
transcription of that gene are specific to the tissue controlled by that enhancer
element. Mutation of the blood cell enhancer does not affect other tissues.
Key signals for a protein coding gene
Promoter region and PolyA addition site
In the diagram below, what are the nucleotides shown in the boxes?
Binding sites for the spliceosome factors
What does myostatin do?
inhibits myogenesis
You want to study the process of development in a relatively unstudied species,
the green anole lizard. The following is the best method for quantifying
expression of as many genes as possible in specific parts of the embryo?
RNA-Seq, assays all genes in genome
ANSWERS
The following is a diagram from Ensembl of the MC1R gene that regulates red
hair color. Where is the translational start site?
A is the transcriptional start site
Diagram of a protein coding gene
The following is a diagram from Ensembl of the MC1R gene that regulates red
hair color. Which of the following represents the 5' UTR?
Diagram of a protein coding gene
(untranslated regions)
What is the genomic signal that tells the cell to start transcription?
the promoter sequences are required to initiate transcription
, Enhancers
they are the sites of transcription factor binding.
Have Flexibility in Their Orientation and Localization
Tissue Specific and Modular
In individuals with the Duffy mutation that increases resistance to malaria, what
level of expression to you expect for the Duffy protein in the endothelial and
Purkinje (brain) cells?
normal; enhancers are tissue-specific. That means that the effects on the
transcription of that gene are specific to the tissue controlled by that enhancer
element. Mutation of the blood cell enhancer does not affect other tissues.
Key signals for a protein coding gene
Promoter region and PolyA addition site
In the diagram below, what are the nucleotides shown in the boxes?
Binding sites for the spliceosome factors
What does myostatin do?
inhibits myogenesis
You want to study the process of development in a relatively unstudied species,
the green anole lizard. The following is the best method for quantifying
expression of as many genes as possible in specific parts of the embryo?
RNA-Seq, assays all genes in genome
