MB ASCP EXAM (MOLECULAR
BIOLOGY ASCP EXAM STUDY GUIDE)
WITH CORRECT SOLUTIONS
The enzyme that primes DNA synthesis is: - ANSWER-DNA primase
Which of the following is a common hydrogen bond acceptor?
a) A carbonyl
b) Primary amine
c) Hydroxyl
d) Tertiary amine - ANSWER-b) Primary amine
What assay is a signal amplification test and uses Alkaline Phosphatase?
a) Branched DNA
b) TMA
c) PCR
d) NASMA
e) LCR - ANSWER-a) Branched DNA
Which one of the following complementary pairings is incorrect?
a) A=T
b) G=C
c) U=T
d) A=U - ANSWER-c) U=T
This highly polymorphic loci region is crucial in assessing immune system compatibility:
- ANSWER-HLA
What does the MSI test detect? - ANSWER-Microsatellite instability
Sickle cell disease is an autosomal genetic disease due to a point mutation in the beta-
globin gene, where glutamic acid is substituted for valine at the sixth codon of the gene,
resulting in a faulty hemoglobin S (Hb S). Sickle cell disease is one of many genetic
diseases where a single gene controls the expression of many phenotypic traits is
referred to as: - ANSWER-Pleiotrophy
How many hydrogen bonds are formed between one A:T base pair? C:G base pair? -
ANSWER-2; 3
, What is the name of the molecule that is added to the 5' end of eukaryotic RNA
transcripts? - ANSWER-GTP
What gene is measured following treatment with imatinib (Gleevec)?
a) FLT3
b) BCR/Abl
c) Jak2
d) MAPK - ANSWER-b) BCR/Able
The bond between a phosphate group and the ribose sugar group in RNA is called
what? - ANSWER-Glycosidic bond
A parent has an autosomal dominant disorder. What percent chance does this parent
have to pass down this affected gene to his/her child? - ANSWER-50%
This phosphate group in a nucleotide is expected to be found on which position of the
sugar?
a) C1'
b) C2'
c) C4'
d) C5' - ANSWER-d) C5'
What genes would be screened in a lung cancer panel? - ANSWER-KRAS, EGFR, ALK
Testing for Aldehyde Dehydrogenase deficiency is important in patients with: -
ANSWER-Alcohol dependence
What is the sequence of the -10 region of prokaryotic promoters? - ANSWER-TATAAT
Deletion in the paternal chromosome 15: del(15)(q11q13) results in Prader-willi
syndrome. However, deletion in the same region in the maternal chromosome results in
a completely different condition known as Angelman syndrome. This phenomenon is an
example of: - ANSWER-Genomic imprinting
What are some characteristics of a melt curve analysis? - ANSWER--At melting point,
the probe separates from the target strand and fluorescence rapidly decreases
-The melting temperature of double stranded DNA depends on its base composition and
length
-All PCR products for a specific primer pair should have the same melting temperature
How is warfarin dosage affected in patients with VKORC1 deficiency? - ANSWER-
Patients receive a lower warfarin dose
You have sequenced a gene and observe the following:
BIOLOGY ASCP EXAM STUDY GUIDE)
WITH CORRECT SOLUTIONS
The enzyme that primes DNA synthesis is: - ANSWER-DNA primase
Which of the following is a common hydrogen bond acceptor?
a) A carbonyl
b) Primary amine
c) Hydroxyl
d) Tertiary amine - ANSWER-b) Primary amine
What assay is a signal amplification test and uses Alkaline Phosphatase?
a) Branched DNA
b) TMA
c) PCR
d) NASMA
e) LCR - ANSWER-a) Branched DNA
Which one of the following complementary pairings is incorrect?
a) A=T
b) G=C
c) U=T
d) A=U - ANSWER-c) U=T
This highly polymorphic loci region is crucial in assessing immune system compatibility:
- ANSWER-HLA
What does the MSI test detect? - ANSWER-Microsatellite instability
Sickle cell disease is an autosomal genetic disease due to a point mutation in the beta-
globin gene, where glutamic acid is substituted for valine at the sixth codon of the gene,
resulting in a faulty hemoglobin S (Hb S). Sickle cell disease is one of many genetic
diseases where a single gene controls the expression of many phenotypic traits is
referred to as: - ANSWER-Pleiotrophy
How many hydrogen bonds are formed between one A:T base pair? C:G base pair? -
ANSWER-2; 3
, What is the name of the molecule that is added to the 5' end of eukaryotic RNA
transcripts? - ANSWER-GTP
What gene is measured following treatment with imatinib (Gleevec)?
a) FLT3
b) BCR/Abl
c) Jak2
d) MAPK - ANSWER-b) BCR/Able
The bond between a phosphate group and the ribose sugar group in RNA is called
what? - ANSWER-Glycosidic bond
A parent has an autosomal dominant disorder. What percent chance does this parent
have to pass down this affected gene to his/her child? - ANSWER-50%
This phosphate group in a nucleotide is expected to be found on which position of the
sugar?
a) C1'
b) C2'
c) C4'
d) C5' - ANSWER-d) C5'
What genes would be screened in a lung cancer panel? - ANSWER-KRAS, EGFR, ALK
Testing for Aldehyde Dehydrogenase deficiency is important in patients with: -
ANSWER-Alcohol dependence
What is the sequence of the -10 region of prokaryotic promoters? - ANSWER-TATAAT
Deletion in the paternal chromosome 15: del(15)(q11q13) results in Prader-willi
syndrome. However, deletion in the same region in the maternal chromosome results in
a completely different condition known as Angelman syndrome. This phenomenon is an
example of: - ANSWER-Genomic imprinting
What are some characteristics of a melt curve analysis? - ANSWER--At melting point,
the probe separates from the target strand and fluorescence rapidly decreases
-The melting temperature of double stranded DNA depends on its base composition and
length
-All PCR products for a specific primer pair should have the same melting temperature
How is warfarin dosage affected in patients with VKORC1 deficiency? - ANSWER-
Patients receive a lower warfarin dose
You have sequenced a gene and observe the following:
