MOLECULAR DIAGNOSTICS PRACTICE
EXAM 1 WITH COMPLETE SOLUTIONS
A virus envelope is comprised of a ______ - ANSWER-phospholipid bilayer
The first __ days of a viral infection are considered the acute phase. - ANSWER-5
Serologic assays detect ____ to viruses. - ANSWER-circulating Ab
In a serologic assay, antibody levels do not correlate with ____. - ANSWER-Level of
infection
What is the gold standard of viral isolation? - ANSWER-cell culture
One costly but sensitive method of virus detection is ____; - ANSWER-Nucleic Acid
Based Detection
What are the 4 families of double stranded DNA viruses? - ANSWER--Adenoviridae
-Herpesviridae
-Papillomaviridae
-Poxviridae
Family of naked ds-DNA viruses that are very stable outside the body - ANSWER-
Adenoviridae
Family of viruses that are associated with the military, viral pink eye, and inadequately
chlorinated pools. - ANSWER-Adenoviridae
What does family Adenoviridae look like in a cell culture? - ANSWER-Swollen grape-like
clusters
What are the 8 species of Herpesviridae? - ANSWER--Herpes Simplex viruses (HSV-1
and HSV-2)
-Varicella-Zoster virus (VZV)
-Epstein-Barr virus (EBV)
-Cytomegalovirus (CMV)
-Human Herpesvirus 6 (HHV-6)
-Human Herpesvirus 7 (HHV-7)
-Human Herpesvirus 8 (HHV-8)
Infections with ____ double the risk of HIV. - ANSWER-Genital Herpes
What is the mortality rate for neonatal herpes? - ANSWER-About 60%
, HSV encephalitis has a ____ mortality rate and is usually caused by ____ in neonates. -
ANSWER-High (70%) HSV-2
What are 5 risks for the recurrence of a herpes family virus? - ANSWER--Sunburn
-Systematic infection
-Immune impairment
-Stress
-Menstruation
How is HSV-1 ad HSV-2 diagnosed in the lab? - ANSWER-Immunofluorescence of
specimens from lesions.
What are the 2 manifestations of Varicella? - ANSWER-Chicken Pox and Shingles
Cells have a distinctive ____appearance in a Tzanck smear. - ANSWER-giant and
multinucleated
What does EBV cause? - ANSWER-Infectious mononucleosis
What virus is Hodgkin lymphoma associated with? - ANSWER-EBV
What viral family is comprised of the smallest ss-DNA viruses? - ANSWER-Parvoviridae
What virus is associated with a "slapped cheek" appearance? - ANSWER-Parvovirus
B19
A fever occurs __-__ days before a rash occurs with Smallpox whereas fever occurs
___ with Chicken Pox. - ANSWER-2-4 at the time of rash
Chicken Pox affects the ___ area of the body whereas Smallpox affects the ___ of the
body first. - ANSWER-Trunk
Limbs
With Smallpox the rash is in the ____ stage(s). - ANSWER-Same
With Chicken Pox the rash occurs in ___ stage(s). - ANSWER-Different
Development of the Smallpox rash is ___and development of the Chicken Pox rash is
____. - ANSWER-Slow
Fast
In otherwise healthy patients ____ results in a transient anemia. - ANSWER-Parvo B19
Parvovirus B19 causes transient aplastic crisis in patients with ____. - ANSWER-Sickle
Cell
EXAM 1 WITH COMPLETE SOLUTIONS
A virus envelope is comprised of a ______ - ANSWER-phospholipid bilayer
The first __ days of a viral infection are considered the acute phase. - ANSWER-5
Serologic assays detect ____ to viruses. - ANSWER-circulating Ab
In a serologic assay, antibody levels do not correlate with ____. - ANSWER-Level of
infection
What is the gold standard of viral isolation? - ANSWER-cell culture
One costly but sensitive method of virus detection is ____; - ANSWER-Nucleic Acid
Based Detection
What are the 4 families of double stranded DNA viruses? - ANSWER--Adenoviridae
-Herpesviridae
-Papillomaviridae
-Poxviridae
Family of naked ds-DNA viruses that are very stable outside the body - ANSWER-
Adenoviridae
Family of viruses that are associated with the military, viral pink eye, and inadequately
chlorinated pools. - ANSWER-Adenoviridae
What does family Adenoviridae look like in a cell culture? - ANSWER-Swollen grape-like
clusters
What are the 8 species of Herpesviridae? - ANSWER--Herpes Simplex viruses (HSV-1
and HSV-2)
-Varicella-Zoster virus (VZV)
-Epstein-Barr virus (EBV)
-Cytomegalovirus (CMV)
-Human Herpesvirus 6 (HHV-6)
-Human Herpesvirus 7 (HHV-7)
-Human Herpesvirus 8 (HHV-8)
Infections with ____ double the risk of HIV. - ANSWER-Genital Herpes
What is the mortality rate for neonatal herpes? - ANSWER-About 60%
, HSV encephalitis has a ____ mortality rate and is usually caused by ____ in neonates. -
ANSWER-High (70%) HSV-2
What are 5 risks for the recurrence of a herpes family virus? - ANSWER--Sunburn
-Systematic infection
-Immune impairment
-Stress
-Menstruation
How is HSV-1 ad HSV-2 diagnosed in the lab? - ANSWER-Immunofluorescence of
specimens from lesions.
What are the 2 manifestations of Varicella? - ANSWER-Chicken Pox and Shingles
Cells have a distinctive ____appearance in a Tzanck smear. - ANSWER-giant and
multinucleated
What does EBV cause? - ANSWER-Infectious mononucleosis
What virus is Hodgkin lymphoma associated with? - ANSWER-EBV
What viral family is comprised of the smallest ss-DNA viruses? - ANSWER-Parvoviridae
What virus is associated with a "slapped cheek" appearance? - ANSWER-Parvovirus
B19
A fever occurs __-__ days before a rash occurs with Smallpox whereas fever occurs
___ with Chicken Pox. - ANSWER-2-4 at the time of rash
Chicken Pox affects the ___ area of the body whereas Smallpox affects the ___ of the
body first. - ANSWER-Trunk
Limbs
With Smallpox the rash is in the ____ stage(s). - ANSWER-Same
With Chicken Pox the rash occurs in ___ stage(s). - ANSWER-Different
Development of the Smallpox rash is ___and development of the Chicken Pox rash is
____. - ANSWER-Slow
Fast
In otherwise healthy patients ____ results in a transient anemia. - ANSWER-Parvo B19
Parvovirus B19 causes transient aplastic crisis in patients with ____. - ANSWER-Sickle
Cell
