MB(ASCP)connect Exam Questions and
Answers
1. Which PCR method has the highest specificity? - Answers -Nested PCR
2. After an amplification procedure followed by gel electrophoresis, you took a photo of
your gel. You notice consistent bands in all your gel lanes. However, you also see what
appears to be a faint but noticeable band in your reagent blank lane. What is your best
explanation for the reagent blank band? - Answers -There was obvious contamination in
this PCR reaction
3. A technologist uses the spectrophotometer to quantify the amount of DNA extracted
from a blood specimen diluted 1:30. The absorbance reading at 260 nm was found to
be 2.545. The concentration of DNA in this extract is: - Answers -3817.5 micrograms/mL
4. This translocation creates a fusion protein between the Abl1 gene on one
chromosome and the BCR gene on the other chromosome, resulting in chronic
myelogenous leukemia (CML): - Answers -t(9;22)
5. The sugar in DNA is: - Answers -Deoxyribose
6. Primer dimers are due to what complimentary issue? - Answers -3' to 3'
complementarity of primer pairs
7. What is the sequence recognized by poly (A) polymerase? - Answers -AAUAAA
8. In what part of a qPCR Amplification Plot do you take your measurement? - Answers
-Exponential phase
9. In CML, what fusion protein is created? - Answers -BCR-Abl1
10. What assay amplifies the probe signal and the probe RNA concentration increases if
the target to be detected is present? - Answers -Qβ replicase
11. Purines and pyrimidines differ from each other in that: - Answers -Purines have two
rings; pyrimidines have one ring
12. The mother of a 3 year old boy took him to the doctor's office for concerns of an
underlying genetic condition. A karyotype order was sent to the Molecular Diagnostic
lab. Karyotype results show 47, XY,+21. Based on these results, the boy has: - Answers
-Down Syndrome
, 13. In a retrovirus the RNA acts as: - Answers -Genome and mRNA
14. The enzyme that primes DNA synthesis is: - Answers -DNA primase
15. What are characteristics of the melt curve analysis? (hint: more than one answer) -
Answers -At the melting point, the probe separates from the target strand and
fluorescence rapidly decreases. The melting temperature of double stranded DNA
depends on its base composition and length. All PCR products for a specific primer pair
should have the same melting temperature.
16. What enzyme is crucial in the making RNA from DNA? - Answers -RNA Polymerase
17. How many polymorphisms can be found in the human genome? - Answers -
Approximately 10 million
18. A DNA specimen was sent to your Molecular Diagnostics Laboratory for DNA
sequencing, in order to determine whether patient A has a particular mutation in gene X.
The gene sequence is known. However, the gene mutation itself is not known.
Therefore, DNA sequencing cannot be performed on this DNA specimen. (True or
False) - Answers -True
19. Mutation in this gene (Xp21) is associated with Duchenne Muscular Dystrophy: -
Answers -Dystrophin
20. Which of the following storage options is optimal for storing isolated DNA for a
period greater than seven years? - Answers --70ºC
22. What is the advantage of Next Generation Sequencing (NGS) over traditional
Sanger Sequencing? - Answers -Ability to sequence multiple mutation variants of a
single gene at a single time
23. Why is it critical to maintain workflow from "clean" to "dirty" while working in a
molecular lab? - Answers -To avoid contamination of specimens with amplified product
which create false positive results
24. What assay amplifies the target using reverse transcriptase? - Answers -TMA
26. A technologist uses the spectrophotometer to quantify the amount of DNA extracted
from a blood specimen diluted 1:30. The absorbance reading at 260 nm was found to
be 2.545. If the absorbance at 280 nm gave a reading of 1.406, and if the the DNA
extract was re-suspended in 0.800 mL of EDTA solution, the DNA yield is: - Answers -
3054 micrograms
27. This restriction enzyme "digests and adds a methyl group from Adenine": - Answers
-Type III
Answers
1. Which PCR method has the highest specificity? - Answers -Nested PCR
2. After an amplification procedure followed by gel electrophoresis, you took a photo of
your gel. You notice consistent bands in all your gel lanes. However, you also see what
appears to be a faint but noticeable band in your reagent blank lane. What is your best
explanation for the reagent blank band? - Answers -There was obvious contamination in
this PCR reaction
3. A technologist uses the spectrophotometer to quantify the amount of DNA extracted
from a blood specimen diluted 1:30. The absorbance reading at 260 nm was found to
be 2.545. The concentration of DNA in this extract is: - Answers -3817.5 micrograms/mL
4. This translocation creates a fusion protein between the Abl1 gene on one
chromosome and the BCR gene on the other chromosome, resulting in chronic
myelogenous leukemia (CML): - Answers -t(9;22)
5. The sugar in DNA is: - Answers -Deoxyribose
6. Primer dimers are due to what complimentary issue? - Answers -3' to 3'
complementarity of primer pairs
7. What is the sequence recognized by poly (A) polymerase? - Answers -AAUAAA
8. In what part of a qPCR Amplification Plot do you take your measurement? - Answers
-Exponential phase
9. In CML, what fusion protein is created? - Answers -BCR-Abl1
10. What assay amplifies the probe signal and the probe RNA concentration increases if
the target to be detected is present? - Answers -Qβ replicase
11. Purines and pyrimidines differ from each other in that: - Answers -Purines have two
rings; pyrimidines have one ring
12. The mother of a 3 year old boy took him to the doctor's office for concerns of an
underlying genetic condition. A karyotype order was sent to the Molecular Diagnostic
lab. Karyotype results show 47, XY,+21. Based on these results, the boy has: - Answers
-Down Syndrome
, 13. In a retrovirus the RNA acts as: - Answers -Genome and mRNA
14. The enzyme that primes DNA synthesis is: - Answers -DNA primase
15. What are characteristics of the melt curve analysis? (hint: more than one answer) -
Answers -At the melting point, the probe separates from the target strand and
fluorescence rapidly decreases. The melting temperature of double stranded DNA
depends on its base composition and length. All PCR products for a specific primer pair
should have the same melting temperature.
16. What enzyme is crucial in the making RNA from DNA? - Answers -RNA Polymerase
17. How many polymorphisms can be found in the human genome? - Answers -
Approximately 10 million
18. A DNA specimen was sent to your Molecular Diagnostics Laboratory for DNA
sequencing, in order to determine whether patient A has a particular mutation in gene X.
The gene sequence is known. However, the gene mutation itself is not known.
Therefore, DNA sequencing cannot be performed on this DNA specimen. (True or
False) - Answers -True
19. Mutation in this gene (Xp21) is associated with Duchenne Muscular Dystrophy: -
Answers -Dystrophin
20. Which of the following storage options is optimal for storing isolated DNA for a
period greater than seven years? - Answers --70ºC
22. What is the advantage of Next Generation Sequencing (NGS) over traditional
Sanger Sequencing? - Answers -Ability to sequence multiple mutation variants of a
single gene at a single time
23. Why is it critical to maintain workflow from "clean" to "dirty" while working in a
molecular lab? - Answers -To avoid contamination of specimens with amplified product
which create false positive results
24. What assay amplifies the target using reverse transcriptase? - Answers -TMA
26. A technologist uses the spectrophotometer to quantify the amount of DNA extracted
from a blood specimen diluted 1:30. The absorbance reading at 260 nm was found to
be 2.545. If the absorbance at 280 nm gave a reading of 1.406, and if the the DNA
extract was re-suspended in 0.800 mL of EDTA solution, the DNA yield is: - Answers -
3054 micrograms
27. This restriction enzyme "digests and adds a methyl group from Adenine": - Answers
-Type III
