MB (ASCP) Exam Questions With
Correct Answers
Predominant form of DNA - AnswerRight-handed B
/.DNA and histone interaction is due to what type of bond? - AnswerIonic
/.What stains to the minor groove of DNA duplex? - AnswerSYBR Green
/.What is the most abundant type of RNA? - AnswerRibosomal RNA (rRNA)
/.What does RNA act as in a retrovirus? - AnswerGenome and mRNA
/.What increases half-life of mRNA? - Answer5' Methyl Cap
/.What kind of DNA is most likely to be transcriptionally active? - AnswerEuchromatin
/.What is the most common type of eukaryotic DNA? - AnswerSatellite DNA
/.What specimen has the most DNA according to molecular weight? - AnswerWhite
Blood Cells
/.What amino acids compose histones? - AnswerLysine and Arginine
/.Amino acid that all proteins start with: - AnswerMethionine
/.What protects the DNA strand during replication? - AnswerSingle-stranded binding
protein
/.Leading Strand - Answer3' to 5' template
/.Lagging Strand - Answer5' to 3' template
/.What DNA strand is being copied in transcription? - AnswerAntisense Strand
/.First step after newly formed mRNA is made? - AnswerAddition of poly(A) tail
/.Every time the chromosome is copied it would become shorter if not for what enzyme?
- AnswerTelomerase
/.What polymerase has repair activity? - AnswerBeta/Epsilon
/.What polymerase acts on leading strand? - AnswerDelta
,/.What polymerase acts on lagging strand? - AnswerAlpha
/.Which polymerase is used for mitochondrial replication and repair? - AnswerGamma
/.What polymerase binds the 5' end to 3' end of dsDNA? - AnswerTopoisomerase
/.Which RNA polymerase is mainly for transcription? - AnswerRNA Pol II
/.Which polymerase is involved with mRNA synthesis? - AnswerRNA Pol II
/.What inactivates RNAses? - AnswerDiethyl pyrocarbonate (DEPC) or bake glassware
for 4 to 6 hours at 400C
/.What type of restriction enzyme recognizes palindromic sites? - AnswerRestriction
Enzyme Type II
/.What type of restriction enzyme lacks methylation activity? - AnswerRestriction
Enzyme Type II
/.What is necessary to make cDNA? - AnswerReverse transcriptase
/.Number of SNPs in a human genome? - Answer11 billion (10^7)
/.How many genes (base pairs) are in a human haploid genome? - Answer3 billion base
pairs
/.The polymerization of acrylamide requires: - AnswerCatalyst (APS or TEMED) or Light
activation
/.How does agarose gel pore size relate to concentration? - AnswerInversely
proportional
/.What is denatured during a southern blot? - AnswerHydrogen bonds (inter-strand
bonds)
/.Melting temperature formula - AnswerTm = (4C x GC Pairs) + (2C x AT pairs)
/.Which is likely to interfere with fluorescent probe? - Answer5' G
/.Proper GC content of primer - Answer35-65%
/.Inhibitors of PCR - AnswerHemoglobin, Heparin, Xylene, Phenol, Cyanol, and
Bromophenol Blue
/.Order to add to a PCR reaction: - AnswerMaster mix > patient sample > positive
control > negative control
, /.Pre Area - AnswerPositive Pressure
/.Post Area - AnswerNegative Pressure
/.Which PCR requires two steps for specificity? - AnswerNested PCR
/.Which microorganism is detected by branched DNA amplification? - AnswerDNA or
RNA
/.Which technique uses RNA, antibodies, and DNA? - AnswerHybrid Capture Assay
/.Use of bisulfite treatment of DNA to determine its pattern of methylation -
AnswerBisulfite DNA
/.What tests are isothermal? - AnswerNASBA, TMA, & SDA
/.Which leukemia is associated with FLT3 gene? - AnswerAML
/.T-cell gene rearrangement is seen in what malignancy? - AnswerSezary Syndrome
/.Which translocation causes Acute Premyelocytic Leukemia? - Answert 15;17
/.Ewing Sarcoma - Answert 11;22
/.Synovial Sarcoma - Answert X;18
/.Follicular Lymphoma - Answert 14;18
/.What genes would be screened in a colorectal panel? - AnswerMSH2 & MLH1
/.What gene mutation causes a high risk of breast or ovarian cancer? - AnswerBRCA1
and BRCA2
/.What does MSI test for? - AnswerMutations in mismatch repair genes (MMR)
/.What disease is related to factor V coagulation gene (F5 1q23)? - AnswerThrombossis
/.How many copies are in a full Fragile X mutation? - Answer200-2000+ CGG repeats
/.What disease is known for numerous mutations and large deletions? -
AnswerMuscular Dystrophy (Duchenne)
/.What HPV causes cervical cancer? - Answer16 & 18
Correct Answers
Predominant form of DNA - AnswerRight-handed B
/.DNA and histone interaction is due to what type of bond? - AnswerIonic
/.What stains to the minor groove of DNA duplex? - AnswerSYBR Green
/.What is the most abundant type of RNA? - AnswerRibosomal RNA (rRNA)
/.What does RNA act as in a retrovirus? - AnswerGenome and mRNA
/.What increases half-life of mRNA? - Answer5' Methyl Cap
/.What kind of DNA is most likely to be transcriptionally active? - AnswerEuchromatin
/.What is the most common type of eukaryotic DNA? - AnswerSatellite DNA
/.What specimen has the most DNA according to molecular weight? - AnswerWhite
Blood Cells
/.What amino acids compose histones? - AnswerLysine and Arginine
/.Amino acid that all proteins start with: - AnswerMethionine
/.What protects the DNA strand during replication? - AnswerSingle-stranded binding
protein
/.Leading Strand - Answer3' to 5' template
/.Lagging Strand - Answer5' to 3' template
/.What DNA strand is being copied in transcription? - AnswerAntisense Strand
/.First step after newly formed mRNA is made? - AnswerAddition of poly(A) tail
/.Every time the chromosome is copied it would become shorter if not for what enzyme?
- AnswerTelomerase
/.What polymerase has repair activity? - AnswerBeta/Epsilon
/.What polymerase acts on leading strand? - AnswerDelta
,/.What polymerase acts on lagging strand? - AnswerAlpha
/.Which polymerase is used for mitochondrial replication and repair? - AnswerGamma
/.What polymerase binds the 5' end to 3' end of dsDNA? - AnswerTopoisomerase
/.Which RNA polymerase is mainly for transcription? - AnswerRNA Pol II
/.Which polymerase is involved with mRNA synthesis? - AnswerRNA Pol II
/.What inactivates RNAses? - AnswerDiethyl pyrocarbonate (DEPC) or bake glassware
for 4 to 6 hours at 400C
/.What type of restriction enzyme recognizes palindromic sites? - AnswerRestriction
Enzyme Type II
/.What type of restriction enzyme lacks methylation activity? - AnswerRestriction
Enzyme Type II
/.What is necessary to make cDNA? - AnswerReverse transcriptase
/.Number of SNPs in a human genome? - Answer11 billion (10^7)
/.How many genes (base pairs) are in a human haploid genome? - Answer3 billion base
pairs
/.The polymerization of acrylamide requires: - AnswerCatalyst (APS or TEMED) or Light
activation
/.How does agarose gel pore size relate to concentration? - AnswerInversely
proportional
/.What is denatured during a southern blot? - AnswerHydrogen bonds (inter-strand
bonds)
/.Melting temperature formula - AnswerTm = (4C x GC Pairs) + (2C x AT pairs)
/.Which is likely to interfere with fluorescent probe? - Answer5' G
/.Proper GC content of primer - Answer35-65%
/.Inhibitors of PCR - AnswerHemoglobin, Heparin, Xylene, Phenol, Cyanol, and
Bromophenol Blue
/.Order to add to a PCR reaction: - AnswerMaster mix > patient sample > positive
control > negative control
, /.Pre Area - AnswerPositive Pressure
/.Post Area - AnswerNegative Pressure
/.Which PCR requires two steps for specificity? - AnswerNested PCR
/.Which microorganism is detected by branched DNA amplification? - AnswerDNA or
RNA
/.Which technique uses RNA, antibodies, and DNA? - AnswerHybrid Capture Assay
/.Use of bisulfite treatment of DNA to determine its pattern of methylation -
AnswerBisulfite DNA
/.What tests are isothermal? - AnswerNASBA, TMA, & SDA
/.Which leukemia is associated with FLT3 gene? - AnswerAML
/.T-cell gene rearrangement is seen in what malignancy? - AnswerSezary Syndrome
/.Which translocation causes Acute Premyelocytic Leukemia? - Answert 15;17
/.Ewing Sarcoma - Answert 11;22
/.Synovial Sarcoma - Answert X;18
/.Follicular Lymphoma - Answert 14;18
/.What genes would be screened in a colorectal panel? - AnswerMSH2 & MLH1
/.What gene mutation causes a high risk of breast or ovarian cancer? - AnswerBRCA1
and BRCA2
/.What does MSI test for? - AnswerMutations in mismatch repair genes (MMR)
/.What disease is related to factor V coagulation gene (F5 1q23)? - AnswerThrombossis
/.How many copies are in a full Fragile X mutation? - Answer200-2000+ CGG repeats
/.What disease is known for numerous mutations and large deletions? -
AnswerMuscular Dystrophy (Duchenne)
/.What HPV causes cervical cancer? - Answer16 & 18
