MB (ASCP) EXAM 2024
ANSWER ALL QUESTIONS IN THIS SECTION
QUESTION 1
Where does the signal come from in pyrosequencing? - ANSWERS-Luciferase
conversion of luciferin to oxyluciferin
QUESTION 2
Primers are usually 18-30 bases, which PCR product should use a shorter primer? -
ANSWERS-Previously amplified product
QUESTION 3
What technique can use RNA or DNA as the starting substrate? - ANSWERS-
bDNA
QUESTION 4
What effect does methylation have on DNA? - ANSWERS-Inhibits transcription
END OF
PAGE 1
, MB (ASCP) EXAM 2024
QUESTION 5
How do you increase the yield of shorter DNA products in PCR? - ANSWERS-
Increase KCl concentration, decrease annealing temperature gradually
QUESTION 6
What is P53? - ANSWERS-Tumor suppressor
QUESTION 7
Which bonds hold together the bases in ssDNA? - ANSWERS-Phosphodiester
bonds
QUESTION 8
Which bonds hold together the nucleotides in dsDNA? - ANSWERS-Hydrogen
bonds
ATG CAG GTG (M Q V)
ATG CAG GTT (M Q V) - ANSWERS-Silent
END OF
PAGE 2
, MB (ASCP) EXAM 2024
ATG CAG GTG (M Q V)
ATG CCG GTG (M P V) - ANSWERS-Missense
ATG CAG GTG (M Q V)
ATG CCG Stop (M Q) - ANSWERS-Nonsense
Protects the sequence of nucleotides, during DNA synthesis this function gives the
enzyme the capacity to proofread. - ANSWERS-Exonuclease
QUESTION 9
Forms phosphodiester bonds between existing DNA strands. - ANSWERS-Ligase
QUESTION 10
Cut and reclose the DNA sugar-phosphate backbone without tangling -
ANSWERS-Helicase
END OF
PAGE 3
, MB (ASCP) EXAM 2024
QUESTION 11
Interconvert topological isomers or relax super twisted DNA - ANSWERS-
Topoisomerase
QUESTION 12
Untangle DNA through double-strand breaks - ANSWERS-Gyrase
QUESTION 13
How many chromosomes are found in interphase? - ANSWERS-46 chromosomes
QUESTION 14
How does higher formamide concentration affect stringency? - ANSWERS-
Increases stringency
QUESTION 15
How does high salt concentration affect stringency? - ANSWERS-Decreases
stringency
END OF
PAGE 4
ANSWER ALL QUESTIONS IN THIS SECTION
QUESTION 1
Where does the signal come from in pyrosequencing? - ANSWERS-Luciferase
conversion of luciferin to oxyluciferin
QUESTION 2
Primers are usually 18-30 bases, which PCR product should use a shorter primer? -
ANSWERS-Previously amplified product
QUESTION 3
What technique can use RNA or DNA as the starting substrate? - ANSWERS-
bDNA
QUESTION 4
What effect does methylation have on DNA? - ANSWERS-Inhibits transcription
END OF
PAGE 1
, MB (ASCP) EXAM 2024
QUESTION 5
How do you increase the yield of shorter DNA products in PCR? - ANSWERS-
Increase KCl concentration, decrease annealing temperature gradually
QUESTION 6
What is P53? - ANSWERS-Tumor suppressor
QUESTION 7
Which bonds hold together the bases in ssDNA? - ANSWERS-Phosphodiester
bonds
QUESTION 8
Which bonds hold together the nucleotides in dsDNA? - ANSWERS-Hydrogen
bonds
ATG CAG GTG (M Q V)
ATG CAG GTT (M Q V) - ANSWERS-Silent
END OF
PAGE 2
, MB (ASCP) EXAM 2024
ATG CAG GTG (M Q V)
ATG CCG GTG (M P V) - ANSWERS-Missense
ATG CAG GTG (M Q V)
ATG CCG Stop (M Q) - ANSWERS-Nonsense
Protects the sequence of nucleotides, during DNA synthesis this function gives the
enzyme the capacity to proofread. - ANSWERS-Exonuclease
QUESTION 9
Forms phosphodiester bonds between existing DNA strands. - ANSWERS-Ligase
QUESTION 10
Cut and reclose the DNA sugar-phosphate backbone without tangling -
ANSWERS-Helicase
END OF
PAGE 3
, MB (ASCP) EXAM 2024
QUESTION 11
Interconvert topological isomers or relax super twisted DNA - ANSWERS-
Topoisomerase
QUESTION 12
Untangle DNA through double-strand breaks - ANSWERS-Gyrase
QUESTION 13
How many chromosomes are found in interphase? - ANSWERS-46 chromosomes
QUESTION 14
How does higher formamide concentration affect stringency? - ANSWERS-
Increases stringency
QUESTION 15
How does high salt concentration affect stringency? - ANSWERS-Decreases
stringency
END OF
PAGE 4
