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Workshop 2-BIOC 3021 Exam Questions
And Answers (Guaranteed A+)
Why does a plot of V vs S taper off and eventually reach a plateau at higher S levels? -
answer✔- When S levels are high, the M-M plot actually approaches a limiting value of Vmax.
- At high (S), where V approximates Vmax, virtually all of the enzyme active sites are occupied
by substrate.
- The addition of more S would not significantly increase the rate of reaction, because the rate
of reaction is limited by the total active sites available; once all the sites are filled, the reaction
can go no faster.
What is the Michaelis-Menten Equation? - answer✔V = Vmax[S]/ [S] +km
The M-M Equation uses the terms (V), (Vmax), (S) and (Km). What do each of these terms
mean? - answer✔V= observable velocity of the reaction. Tells us how rapidly product (p) is
being formed by the reaction.
Vmax = fastest reaction rate possible under the given assay conditions. Has dimensions of
change in product concentration per unit time.
S = substrate concentration
Km = Michaelis Constant. Has units of concentration
What does the Km term tell us about an enzyme? - answer✔The Km tells us how efficient
enzymes are at low substrate levels
What does it signify if an enzyme has a low Km or a high Km? - answer✔- The Km tells us how
efficient enzymes are at low substrate levels?
- At low Km, the enzyme is very efficient at low substrate concentration. At high Km, enzymes
are inefficient at low substrate concentration.
What is the enzyme rate when the concentration of S = Km? - answer✔1/2 Vmax.
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ALL RIGHTS RESERVED.
Workshop 2-BIOC 3021 Exam Questions
And Answers (Guaranteed A+)
Why does a plot of V vs S taper off and eventually reach a plateau at higher S levels? -
answer✔- When S levels are high, the M-M plot actually approaches a limiting value of Vmax.
- At high (S), where V approximates Vmax, virtually all of the enzyme active sites are occupied
by substrate.
- The addition of more S would not significantly increase the rate of reaction, because the rate
of reaction is limited by the total active sites available; once all the sites are filled, the reaction
can go no faster.
What is the Michaelis-Menten Equation? - answer✔V = Vmax[S]/ [S] +km
The M-M Equation uses the terms (V), (Vmax), (S) and (Km). What do each of these terms
mean? - answer✔V= observable velocity of the reaction. Tells us how rapidly product (p) is
being formed by the reaction.
Vmax = fastest reaction rate possible under the given assay conditions. Has dimensions of
change in product concentration per unit time.
S = substrate concentration
Km = Michaelis Constant. Has units of concentration
What does the Km term tell us about an enzyme? - answer✔The Km tells us how efficient
enzymes are at low substrate levels
What does it signify if an enzyme has a low Km or a high Km? - answer✔- The Km tells us how
efficient enzymes are at low substrate levels?
- At low Km, the enzyme is very efficient at low substrate concentration. At high Km, enzymes
are inefficient at low substrate concentration.
What is the enzyme rate when the concentration of S = Km? - answer✔1/2 Vmax.
1|Page
