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ADVANCED BIOCHEMISTRY TEST QUESTIONS AND ANSWERS 2025

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ADVANCED BIOCHEMISTRY TEST QUESTIONS AND ANSWERS 2025...

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What are the reactants, enzymes and products involved in the first reaction of
glycolysis? - ANSWER Reactant: D-Glucose + ATP

Catalysized Enzyme: Hexokinase
(can also use D-fructose and D-Mannose) w/ Mg2+

Products: Glucose-6-phosphate (G6P) + ADP + H+

What are the reactants, enzymes and products involved in the second reaction of
glycolysis? - ANSWER Reactant: Glucose-6-phosphate (G6P)

Catalysized Enzyme: phosphoglucose isomerase (PGI)

Products: Fructose-6-phosphate (F6P)

What are the reactants, enzymes and products involved in the third reaction of
glycolysis? - ANSWER Reactant: Fructose-6-phosphate (F6P) + ATP

Catalysized Enzyme: phosphofructokinase (PFK) w/Mg2+

Products: Fructose-1,6-biphosphate (FBP) + ADP + H+

What are the reactants, enzymes and products involved in the forth reaction of
glycolysis. - ANSWER Reactant: Fructose-1,6-biphosphate (F6P)

Catalysized Enzyme: Aldolase

Products: Glyceraldehyde-3-phosphate (GAP) + Dihydroxyacetone phosphate
(DHAP)

What are the reactants, enzymes and products involved in the five reaction of
glycolysis. - ANSWER Reactant: Glyceraldehyde-3-phosphate (GAP) +
Dihydroxyacetone phosphate (DHAP)

, Catalysized Enzyme: Triose phosphate isomerase (TPI)

Products: 2x Glyceraldehyde-3-phosphate (GAP)

What are the reactants, enzymes and products involved in the six reaction of
glycolysis. - ANSWER START TO STAGE 2: Net -2 ATP currently

First oxidative-reduction reaction and
"high energy" acyl group

Reactant: 2x Glyceraldehyde-3-phosphate (GAP) + NAD+ + Pi

Catalyst Enzyme: Glyceraldehyde-3-phosphate
dehydrogenase (GAPDH)


Products: 2x 1,3-Bisphosphoglycerate (1,3-BPG) + NADH + H+

What are the reactants, enzymes and products involved in the seven reaction of
glycolysis. - ANSWER Reactant: 2x 1,3-Bisphosphoglycerate (1,3-BPG) +
ADP

Catalyst Enzyme: phosphoglycerate kinase (PGK)


Products: 2x 3-phosphoglycerate (3PG) + ATP

Net 0 ATP currently

What are the reactants, enzymes and products involved in the eighth reaction of
glycolysis. - ANSWER Reactant: 2x phosphoglycerate mutase (PGM)

Catalyst Enzyme: phosphoglycerate mutase (PGM)


Products: 2x 3-phosphoglycerate (3PG) + ATP

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