Polymerase Chain Reaction Lab Review 2024/2025 with 100% correct answers

Polymerase Chain Reaction Lab Review

We are going to use the blood sample you collected at the crime scene. What needs to happen before
the blood sample can be used for PCR? a) DNA has to be isolated from the cells b) No action is needed c)
You should shake the tube d) The blood has to be frozen. correct answersa) DNA has to be isolated from
the cells



What do you need to do each time before using pipette to collect liquid? a) Put on a used pipette tip b)
Polish the pipette c) Shake the pipette d) Put on a new, sterile pipette tip correct answersd) Put on a new,
sterile pipette tip



What is the function of primers in a PCR reaction? a) Denature DNA b) Bind specific sites on the DNA c)
Bind random sites on the DNA d) Copy DNA correct answersb) Bind specific sites on the DNA



Why is it important to change the pipette tip? a) To employ more garbage men b) To avoid cross
contamination c) To keep the lab bench clean d) To keep the lab assistant happy correct answersb) To
avoid cross contamination



For which enzyme are nucleotides the substrate? a) DNA polymerase b) Ligase c) Protease d) Ribosome
correct answersa) DNA polymerase



What is the template of the PCR reaction? a) DNA b) RNA c) Nucleotides d) Proteins correct answersa)
DNA



What does a DNA polymerase do? a) Degrades proteins b) Unfolds DNA c) Synthesizes DNA d) Cleaves
DNA correct answersc) Synthesizes DNA



At this step in the PCR process, (95°C) what happens to the DNA? a) It is kept intact b) It will be
separated into two strands c) It will be twisted into double helix d) It will be broken into many pięces
correct answersb) It will be separated into two strands

, How is the DNA separated into single strands? a) The DNA polymerase separating the two DNA strands
b) The high temperature (95 °C) c) The primers separating the two DNA strands d) The low temperature
(54 °C) correct answersb) The high temperature (95 °C)



The area where the primers bind marks which part of the PCR product? a) End b) Beginning C) Left side
d) Right side correct answersb) Beginning



The PCR products get a certain length due to whích fact? a) The DNA breaking off b) The DNA
polymerase falling off C) The heat in the PCR machine d) The placement of the primers correct answersd)
The placement of the primers



How does the DNA polymerase extend the primers into a new DNA strand? a) Adding nucleotides to the
3' and 5' ends of the primers b) Adding nucleotides to the 3' end of the primers c) Adding more primers
to the strand d) Adding nucleotides to the 5' end of the primers correct answersb) Adding nucleotides to
the 3' end of the primers



Primers are always designed to be complementary to the template DNA strand. Which of these
sequences is the complementary sequence to the template sequence 5'-GTGGTCTGATCAACGGTAA-3'? a)
3'-GTGGTCTGATCAACGGTAA-5' b) 3'-CACCAGACTAGTTGCCATT-5' c) 5'-CACCAGACTAGTTGCCATT-3' d) 5'-
GTGGTCTGATCAACGGTAA-3' correct answersb) 3'-CACCAGACTAGTTGCCATT-5'



STR analysis correct answersDifferent length may found in each individual. Since individuals might have
different numbers of repeats creating their own specific DNA profiles.



What happens to the probability of a 100% match between two different individuals when using 13 sets
of primers for the DNA profile instead of one? a) It decreases b) It results in a match c) It increases d) It is
not affected correct answersa) It decreases



How many copies of DNA are required to see bands on the electrophoresis gel? a) 1000 copies b) 10
copies c) Millions of copies d) None correct answersc) Millions of copies



Which word describes the charge of the DNA? a) Positively charged b) Not charged c) Highly charged d)
Negatively charged correct answersd) Negatively charged