Pearson Edexcel Advanced Level GCE In Biology B
9BI0)Paper 03: General and Practical Principles in
Biology
Pearson Edexcel Advanced Leve
GCE In Biology B (9BI0)Paper 03
General and Practical Principles i
Biology
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Paper
B
■ ■
Advanced
PAPER 3: General and Practical Principles in Biology
Marks
Scientific calculator, HB pencil, ruler
Instructions
• Use black ink or ball‑point pen.
centre number and candidate number.
Answer the questions in the spaces provided –
there may be more space than you need.
Information
• The total mark for this paper is 120.
– use this as a guide as to how much time to spend on each question.
In question(s) marked with an asterisk (*), marks will be awarded for your ability to
structure your answer logically, showing how the points that you make are related or
follow on from each other where appropriate
Advice
• Read each question carefully before you start to answer it.
• Check your answers if you have time at the end.
Turn over
, Answer ALL questions.
1 A student made a root tip squash to observe the stages of mitosis.
(a) Name the region of the root tip where cells are actively dividing.
(1)
. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . .. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . . .. . . . .. . . . .. . . .. . . . .. . .. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . .. . . . . . .. . . .. . . . .. . . . .. ......................
(b) Give one reason for each of the following:
(i) Treating the root tip with strong acid
(1)
. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . .. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . . .. . . . .. . . . .. . . .. . . . .. . .. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . .. . . . . . .. . . .. . . . .. . . . .. ......................
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(ii) Adding a stain to the root tip
(1)
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(iii) Pressing lightly on the coverslip
(1)
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2
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, (c) The diagram shows cells from a root tip squash. The
total number of cells is 39.
Calculate the mitotic index for this root tip squash. Use
the formula
number of cells in mitosis
Mitotic index = × 100
total number of cells
Give your answer to one decimal place.
(2)
Answer ..............................................................
(Total for Question 1 = 6 marks)
3
■■■■ Turn over
9BI0)Paper 03: General and Practical Principles in
Biology
Pearson Edexcel Advanced Leve
GCE In Biology B (9BI0)Paper 03
General and Practical Principles i
Biology
[Type the company name]
[Type the company address]
[Type the phone number]
, Candidate surname Other names
Centre Number Candidate Number
Paper
B
■ ■
Advanced
PAPER 3: General and Practical Principles in Biology
Marks
Scientific calculator, HB pencil, ruler
Instructions
• Use black ink or ball‑point pen.
centre number and candidate number.
Answer the questions in the spaces provided –
there may be more space than you need.
Information
• The total mark for this paper is 120.
– use this as a guide as to how much time to spend on each question.
In question(s) marked with an asterisk (*), marks will be awarded for your ability to
structure your answer logically, showing how the points that you make are related or
follow on from each other where appropriate
Advice
• Read each question carefully before you start to answer it.
• Check your answers if you have time at the end.
Turn over
, Answer ALL questions.
1 A student made a root tip squash to observe the stages of mitosis.
(a) Name the region of the root tip where cells are actively dividing.
(1)
. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . .. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . . .. . . . .. . . . .. . . .. . . . .. . .. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . .. . . . . . .. . . .. . . . .. . . . .. ......................
(b) Give one reason for each of the following:
(i) Treating the root tip with strong acid
(1)
. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . .. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . . .. . . . .. . . . .. . . .. . . . .. . .. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . .. . . . . . .. . . .. . . . .. . . . .. ......................
. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . .. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . . .. . . . .. . . . .. . . .. . . . .. . .. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . .. . . . . . .. . . .. . . . .. . . . .. ......................
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(ii) Adding a stain to the root tip
(1)
. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . .. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . . .. . . . .. . . . .. . . .. . . . .. . .. . . . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . .. . . . .. . . . .. . . . .. . . .. . .. . . . . . .. . . .. . . . .. . . . .. ......................
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(iii) Pressing lightly on the coverslip
(1)
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2
■■■■
, (c) The diagram shows cells from a root tip squash. The
total number of cells is 39.
Calculate the mitotic index for this root tip squash. Use
the formula
number of cells in mitosis
Mitotic index = × 100
total number of cells
Give your answer to one decimal place.
(2)
Answer ..............................................................
(Total for Question 1 = 6 marks)
3
■■■■ Turn over
