MB (ASCP) (2024-2025) EXAM QUESTIONS WITH
ANSWERS
Which microorganism is detected by branched DNA
amplification? - <<<Answers>>>DNA or RNA
Which technique uses RNA, antibodies, and DNA? -
<<<Answers>>>Hybrid Capture Assay
Use of bisulfite treatment of DNA to determine its pattern of
methylation - <<<Answers>>>Bisulfite DNA
Multiplex PCR: short PCR products are weak -
<<<Answers>>>Decrease annealing time
Decrease annealing temperature
Decrease extension time
Decrease extension temperature
Increase KCl concentration
Decrease denaturing time
Multiplex PCR: longer pcr products are weak -
<<<Answers>>>Increase annealing time
Decrease annealing temperature
Increase extension time
Increase extension temperature
Decrease KCl concentration
Increase denaturing time
, MB (ASCP) (2024-2025) EXAM QUESTIONS WITH
ANSWERS
Metacentric - <<<Answers>>>
Submetacentric - <<<Answers>>>
Acrocentric - <<<Answers>>>
Telocentric - <<<Answers>>>
How many chromosomes during M Phase (end of mitosis)? -
<<<Answers>>>46 chromosomes
How many chromosomes during G1 Phase? -
<<<Answers>>>46 chromosomes
What happens during S Phase? - <<<Answers>>>DNA is
replicated
How many chromosomes during G2 Phase? -
<<<Answers>>>92 chromosomes
rRNA small subunit (prokaryotes) - <<<Answers>>>16s
rRNA large subunit (prokaryotes) - <<<Answers>>>23s & 5s
, MB (ASCP) (2024-2025) EXAM QUESTIONS WITH
ANSWERS
rRNA small subunit (eukaryotes) - <<<Answers>>>18s
rRNA large subunit (eukaryotes) - <<<Answers>>>5.8s &
28s
Long stretches of noncoding DNA in newly transcribed
mRNA - <<<Answers>>>Introns
Newly transcribed mRNA that contains introns which are
removed by splicing - <<<Answers>>>Heteronuclear RNA
(hnRNA)
Remaining sequences of mRNA that code for protein products
- <<<Answers>>>Exons
Phenotype will be present in individuals w/ one or both
chromosomes mutated - <<<Answers>>>Autosomal-
dominant
Likelihood of the disease phenotype in offspring is 25%
indicating the presence of the recessive mutation in at least
one chromosome in each of the parents -
<<<Answers>>>Autosomal-recessive
ANSWERS
Which microorganism is detected by branched DNA
amplification? - <<<Answers>>>DNA or RNA
Which technique uses RNA, antibodies, and DNA? -
<<<Answers>>>Hybrid Capture Assay
Use of bisulfite treatment of DNA to determine its pattern of
methylation - <<<Answers>>>Bisulfite DNA
Multiplex PCR: short PCR products are weak -
<<<Answers>>>Decrease annealing time
Decrease annealing temperature
Decrease extension time
Decrease extension temperature
Increase KCl concentration
Decrease denaturing time
Multiplex PCR: longer pcr products are weak -
<<<Answers>>>Increase annealing time
Decrease annealing temperature
Increase extension time
Increase extension temperature
Decrease KCl concentration
Increase denaturing time
, MB (ASCP) (2024-2025) EXAM QUESTIONS WITH
ANSWERS
Metacentric - <<<Answers>>>
Submetacentric - <<<Answers>>>
Acrocentric - <<<Answers>>>
Telocentric - <<<Answers>>>
How many chromosomes during M Phase (end of mitosis)? -
<<<Answers>>>46 chromosomes
How many chromosomes during G1 Phase? -
<<<Answers>>>46 chromosomes
What happens during S Phase? - <<<Answers>>>DNA is
replicated
How many chromosomes during G2 Phase? -
<<<Answers>>>92 chromosomes
rRNA small subunit (prokaryotes) - <<<Answers>>>16s
rRNA large subunit (prokaryotes) - <<<Answers>>>23s & 5s
, MB (ASCP) (2024-2025) EXAM QUESTIONS WITH
ANSWERS
rRNA small subunit (eukaryotes) - <<<Answers>>>18s
rRNA large subunit (eukaryotes) - <<<Answers>>>5.8s &
28s
Long stretches of noncoding DNA in newly transcribed
mRNA - <<<Answers>>>Introns
Newly transcribed mRNA that contains introns which are
removed by splicing - <<<Answers>>>Heteronuclear RNA
(hnRNA)
Remaining sequences of mRNA that code for protein products
- <<<Answers>>>Exons
Phenotype will be present in individuals w/ one or both
chromosomes mutated - <<<Answers>>>Autosomal-
dominant
Likelihood of the disease phenotype in offspring is 25%
indicating the presence of the recessive mutation in at least
one chromosome in each of the parents -
<<<Answers>>>Autosomal-recessive
