NSCI 175 Final Exam Study Guide
Immunocytochemistry - Answer-assesses gene expression at the protein level
Immunohistochemistry - Answer-Assesses gene expression at the protein level. Uses antibodies to test
for certain antigens in a sample tissue.
In-situ Hybridization - Answer-mRNA level. allows for precise localization of a specific segment of
nucleic acid using an mRNA probe with the complementary strand.
Describe how CRE Technology works and how it can be used to identify the structure and function of
neurons - Answer-Cre-recombinase removes genes from area within Lox-P regions. This gene can be
inserted elsewhere OR the removal of gene can cause side effects which can be studied
Neurons or not? Olfactory cells and Gustatory Taste Receptors - Answer-olfactory cells = neurons
taste cells = NOT neurons
most of your neurons are in you: - Answer-cerebellum
, what happens to cell if you increase extracellular K+? - Answer-cell depolarizes
AMPA receptors are: - Answer-ligand-gated
NMDA receptors are - Answer-ligand and voltage-gated
What type of receptive fields to bipolar and ganglion cells have? - Answer-concentric (circular with a
center and surround_
non-thalamic targets of the optic tract and their functions - Answer-hypothalamus: biological rhythms
pretectum: controls pupil size; types of eye movement
superior colliculus: orients eyes in response to new stimuli
Inputs of striate cortex - Answer-Magno LGN (layers 1& 2) --> IVC alpha
Parvo LGN (layers 3-6) --> IVC beta
Koniocellular LGN (K1-K6) --> Layers I-III
Outputs of striate cortex - Answer-Layers II, III, IVB --> other cortical areas
Layer V --> superior colliculus and pons
Layer VI --> project back to LGN
dorsal stream - Answer-how and where pathway: visual motion analysis and visual control of action
-roles: navigation, directing eye movements, motion perception
-neurons similar to magnocellular neurons in V1
-areas: V2, V3, MT
- receptive fields selective for motion
Immunocytochemistry - Answer-assesses gene expression at the protein level
Immunohistochemistry - Answer-Assesses gene expression at the protein level. Uses antibodies to test
for certain antigens in a sample tissue.
In-situ Hybridization - Answer-mRNA level. allows for precise localization of a specific segment of
nucleic acid using an mRNA probe with the complementary strand.
Describe how CRE Technology works and how it can be used to identify the structure and function of
neurons - Answer-Cre-recombinase removes genes from area within Lox-P regions. This gene can be
inserted elsewhere OR the removal of gene can cause side effects which can be studied
Neurons or not? Olfactory cells and Gustatory Taste Receptors - Answer-olfactory cells = neurons
taste cells = NOT neurons
most of your neurons are in you: - Answer-cerebellum
, what happens to cell if you increase extracellular K+? - Answer-cell depolarizes
AMPA receptors are: - Answer-ligand-gated
NMDA receptors are - Answer-ligand and voltage-gated
What type of receptive fields to bipolar and ganglion cells have? - Answer-concentric (circular with a
center and surround_
non-thalamic targets of the optic tract and their functions - Answer-hypothalamus: biological rhythms
pretectum: controls pupil size; types of eye movement
superior colliculus: orients eyes in response to new stimuli
Inputs of striate cortex - Answer-Magno LGN (layers 1& 2) --> IVC alpha
Parvo LGN (layers 3-6) --> IVC beta
Koniocellular LGN (K1-K6) --> Layers I-III
Outputs of striate cortex - Answer-Layers II, III, IVB --> other cortical areas
Layer V --> superior colliculus and pons
Layer VI --> project back to LGN
dorsal stream - Answer-how and where pathway: visual motion analysis and visual control of action
-roles: navigation, directing eye movements, motion perception
-neurons similar to magnocellular neurons in V1
-areas: V2, V3, MT
- receptive fields selective for motion
