QBM exam| Questions Solved with Verified
Correct Answers | Latest Update 2024-
2025
Genome walking uses ___________, and shotgun sequencing uses ___________.
nested PCR; helicase-dependent PCR
anchored PCR; TA cloning
asymmetric PCR; multiplex PCR
inverse PCR; RAPD PCR
qPCR; RT-PCR - ANSWER anchored PCR; TA cloning
Which type of next generation DNA sequencing involves adding one base at a time
and detecting the release of hydrogens from DNA synthesis? - ANSWER Ion
semiconductor sequencing.
Based on the restriction enzyme buffer table below, which of the following is TRUE?
A gel extraction must be performed before digesting DNA with HpaI if EcoRI in Buffer 1 was
used.
BamHI and HpaI can efficiently cut DNA together in Buffer 4.
EcoRI and XhoI should ideally be used together in Buffer 1 or Buffer 2.
XbaI and EcoRI can't be used to digest DNA at the same time in Buffer 1.
XbaI and BamHI should ideally be used together in Buffer 2. - ANSWER XbaI and BamHI should
ideally be used together in Buffer 2.
,You wish to use the plasmid below to generate a construct. Your goal is to insert a gene into
this plasmid, grow the cells on ampicillin, and select colonies with blue/white screening.
Which restriction enzymes could you use?
Nco1 and Sal1.
Not1 and Nco1.
EcoR1 and Xho1.
Pst1 and Xho1.
EcoR1 only. - ANSWER Nco1 and Sal1.
Patient-specific chemotherapy, such as aggressive or non-aggressive treatment, can be best
decided by
a Southern blot.
VNTR data.
RT-PCR data.
RFLP data.
microarray data. - ANSWER microarray data.
What primers would you design to amplify the following sequence of DNA?
5'-AGCAGTTGAGCAGTTGAGTACTTAGGCCTGCAGTGTACTTAG-3'
Forward: 5'-AGCAG-3' Reverse: 5'-CTAAG-3'
Forward: 5'-CGTCT-3' Reverse: 5'-GAATC-3'
Forward: 5'-GACGA-3' Reverse: 5'-CTTAG-3'
Forward: 5'-CTGCT-3' Reverse: 5'-CTAAG-3'
,Forward: 5'-AGCAG-3' Reverse: 5'-CTTAG-3' - ANSWER Forward: 5'-AGCAG-3' Reverse: 5'-
CTAAG-3'
EDTA does NOT inhibit _____ activity - ANSWER RNase activity
Which lane below represents a digestion with PstI and XbaI?
Lane 2
Lane 3
Lane 1
Lane 4
Lane 5 - ANSWER LANE 5
The gel below shows a paternity test using VNTRs via Southern blotting. Which DNA sample is
from the child's true father?
B
E
D
C
A - ANSWER D
Which of the following is usually performed as part of a Southern blot?
protein transfer to a membrane.
Probe hybridization to double-stranded DNA.
Sample digestion by restriction enzymes.
Cross-hybridization of DNA-based antibodies.
All answer choices are correct. - ANSWER Sample digestion by restriction enzymes.
, In the future, DNA sequencing may be performed via
All answer choices are correct.
mass spectrometry.
DNA chips.
DNA microarrays. - ANSWER all answers
In the melt curve below,
qpcr.jpg
the qPCR sample is sufficiently pure.
a cycle threshold line is shown.
the qPCR sample is contaminated with nonspecific DNA.
absorbance is measured at 280 nm.
the qPCR sample is contaminated with various proteins. - ANSWER the qPCR sample
is contaminated with nonspecific DNA.
You use the following primer in a PCR reaction: A G G C T T T C G G A G T What
annealing temperature should you use in a thermocycler for this primer?
50 °C
45 °C
35 °C
55 °C
40 °C - ANSWER 35
Correct Answers | Latest Update 2024-
2025
Genome walking uses ___________, and shotgun sequencing uses ___________.
nested PCR; helicase-dependent PCR
anchored PCR; TA cloning
asymmetric PCR; multiplex PCR
inverse PCR; RAPD PCR
qPCR; RT-PCR - ANSWER anchored PCR; TA cloning
Which type of next generation DNA sequencing involves adding one base at a time
and detecting the release of hydrogens from DNA synthesis? - ANSWER Ion
semiconductor sequencing.
Based on the restriction enzyme buffer table below, which of the following is TRUE?
A gel extraction must be performed before digesting DNA with HpaI if EcoRI in Buffer 1 was
used.
BamHI and HpaI can efficiently cut DNA together in Buffer 4.
EcoRI and XhoI should ideally be used together in Buffer 1 or Buffer 2.
XbaI and EcoRI can't be used to digest DNA at the same time in Buffer 1.
XbaI and BamHI should ideally be used together in Buffer 2. - ANSWER XbaI and BamHI should
ideally be used together in Buffer 2.
,You wish to use the plasmid below to generate a construct. Your goal is to insert a gene into
this plasmid, grow the cells on ampicillin, and select colonies with blue/white screening.
Which restriction enzymes could you use?
Nco1 and Sal1.
Not1 and Nco1.
EcoR1 and Xho1.
Pst1 and Xho1.
EcoR1 only. - ANSWER Nco1 and Sal1.
Patient-specific chemotherapy, such as aggressive or non-aggressive treatment, can be best
decided by
a Southern blot.
VNTR data.
RT-PCR data.
RFLP data.
microarray data. - ANSWER microarray data.
What primers would you design to amplify the following sequence of DNA?
5'-AGCAGTTGAGCAGTTGAGTACTTAGGCCTGCAGTGTACTTAG-3'
Forward: 5'-AGCAG-3' Reverse: 5'-CTAAG-3'
Forward: 5'-CGTCT-3' Reverse: 5'-GAATC-3'
Forward: 5'-GACGA-3' Reverse: 5'-CTTAG-3'
Forward: 5'-CTGCT-3' Reverse: 5'-CTAAG-3'
,Forward: 5'-AGCAG-3' Reverse: 5'-CTTAG-3' - ANSWER Forward: 5'-AGCAG-3' Reverse: 5'-
CTAAG-3'
EDTA does NOT inhibit _____ activity - ANSWER RNase activity
Which lane below represents a digestion with PstI and XbaI?
Lane 2
Lane 3
Lane 1
Lane 4
Lane 5 - ANSWER LANE 5
The gel below shows a paternity test using VNTRs via Southern blotting. Which DNA sample is
from the child's true father?
B
E
D
C
A - ANSWER D
Which of the following is usually performed as part of a Southern blot?
protein transfer to a membrane.
Probe hybridization to double-stranded DNA.
Sample digestion by restriction enzymes.
Cross-hybridization of DNA-based antibodies.
All answer choices are correct. - ANSWER Sample digestion by restriction enzymes.
, In the future, DNA sequencing may be performed via
All answer choices are correct.
mass spectrometry.
DNA chips.
DNA microarrays. - ANSWER all answers
In the melt curve below,
qpcr.jpg
the qPCR sample is sufficiently pure.
a cycle threshold line is shown.
the qPCR sample is contaminated with nonspecific DNA.
absorbance is measured at 280 nm.
the qPCR sample is contaminated with various proteins. - ANSWER the qPCR sample
is contaminated with nonspecific DNA.
You use the following primer in a PCR reaction: A G G C T T T C G G A G T What
annealing temperature should you use in a thermocycler for this primer?
50 °C
45 °C
35 °C
55 °C
40 °C - ANSWER 35
