DNA sequencing and DNA profiling Exam Questions and Answers Already Passed

DNA sequencing and DNA profiling Exam Questions and Answers Already Passed Restriction enzyme - Answers Scissors Cuts the DNA at a specific sequence probes - Answers Small pieces of DNA that are radioactive and will glow Helps identify DNA Used to find silicates between two DNA strands Polymerase chain reaction - Answers A method to quickly copy a small DNA sample DNA profiling can be used for what - Answers Crime Paternity test Disease Gel Electrophoresis - Answers Separates DNA fragments by size Paternity test requires an exact match or half and half - Answers Half and half Crime requires an exact match or half and half - Answers Exact Disease requires an exact match or half and half - Answers Exact Recognition site - Answers Where the retraction enzyme cuts Recombinant DNA - Answers DNA from two or more different organism is combined Steps of Recombinant DNA - Answers Treat DNA from both sources with restriction enzyme The enzyme will cut both molecules so they have complementary sticky ends Two pieces of DNA will be linked by DNA ligase Recombinant DNA is made Which part of bacteria is used in recombinant DNA - Answers Plasmid a.k.a the circular DNA How is bacteria used in recombinant DNA - Answers The plasmid of bacteria is usually used since the bacteria don't really need it and since testing on bacteria is better than testing on animals Recombinant DNA uses - Answers Making blood proteins, hormones, vaccines, pharmaceuticals Cloning genes Gene therapy DNA profiling - Answers DNA is removed and cut with the restriction enzyme DNA is mixed with radioactive probes DNA is put on a well of the gel The gel is put in an electrophoresis chamber with the wells at a negative end Electricity is run through the gel and the DNA moves towards the positive end (DNA is negative and opposites attract) Probed pieces glow on the gel The _______ the molecule the farther down it will move because it's ________ - Answers Smaller faster What is used to find the sequence of nucleotides in a nuclei acid - Answers DNA profiling DNA profiling uses - Answers Diagnosis and treatment of disease Gene therapy Agriculture (GMOs) Restriction enzyme - Answers Scissors Cuts the DNA at a specific sequence probes - Answers Small pieces of DNA that are radioactive and will glow Helps identify DNA Used to find silicates between two DNA strands Polymerase chain reaction - Answers A method to quickly copy a small DNA sample DNA profiling can be used for what - Answers Crime Paternity test Disease Gel Electrophoresis - Answers Separates DNA fragments by size Paternity test requires an exact match or half and half - Answers Half and half Crime requires an exact match or half and half - Answers Exact Disease requires an exact match or half and half - Answers Exact Recognition site - Answers Where the retraction enzyme cuts Recombinant DNA - Answers DNA from two or more different organism is combined Steps of Recombinant DNA - Answers Treat DNA from both sources with restriction enzyme The enzyme will cut both molecules so they have complementary sticky ends Two pieces of DNA will be linked by DNA ligase Recombinant DNA is made Which part of bacteria is used in recombinant DNA - Answers Plasmid a.k.a the circular DNA How is bacteria used in recombinant DNA - Answers The plasmid of bacteria is usually used since the bacteria don't really need it and since testing on bacteria is better than testing on animals