MB (ASCP) Exam NEWEST 2024 WITH COMPLETE
QUESTIONS AND CORRECT VERIFIED ANSWERS
(DETAILED ANSWERS) ALREADY GRADED A+ 100%
GUARANTEED TO PASS CONCEPTS!!!
Predominant form of DNA - ✔✔ANSW✔✔..Right-handed B
DNA and histone interaction is due to what type of bond? - ✔✔ANSW✔✔..Ionic
DNA methyltransferases - ✔✔ANSW✔✔..Prokaryotic DNA: mostly methylated or
hemimethylated. Asa means to differentiate host DNA from non-host and to provide
resistance to restriction enzymes. Eukaryotic DNA: is methylated in specific regions.
DNA- binding proteins may limit accessibility or guide methyltransferases to specific
regions of the DNA
DNA methytransferases catalyze the addition of methyl groups to nitrogen bases,
usually adenines and cytosines in DNA strands.
How does genetic information move in the conjugation process? - ✔✔ANSW✔✔..From
F+ to F- bacteria. F+ has a fertility factor that not only carried the information from one
cell to another but also was responsible for establishing hte physical connection
between the mating bacteria.
F factor (fertility factor) - ✔✔ANSW✔✔..extrachromosomal circle of double-stranded
DNA or plasmid carrying the genes coding for construction of the mating bridge. One
copy of the F factor remains in the F+ Bacteria and the other is sent into the F- Bacteria.
Protease. and Ribonuclease - ✔✔ANSW✔✔..degrades protein and RNA
Different physical states of the plasmid DNA CAN BE RESOLVED BY distinct _____
characteristcs during gel electrophoresis - ✔✔ANSW✔✔..migration
,What was the first indication of the episomal (plasmid) nature of the resistance factor? -
✔✔ANSW✔✔..Multiple drug resistance in bacteria can be eliminated by treatment with
acridine dyes
RTF or R factors - ✔✔ANSW✔✔..In resistant bacteria, plasmids carrying the genes for
inactivation or circumvention of antiobiotic action were called resistance factors.
Gene Expression - ✔✔ANSW✔✔..Inorder for DNA info to be utilized, it must be
transcribed and then translated into protein
Which RNA carries the info in DNA to hte ribosomes where it is translated into a
protein? - ✔✔ANSW✔✔..mRNA
Transcription - ✔✔ANSW✔✔..the copying of one strand of DNA into RNA by a process
similar to that of DNA replication
Transcription is catalyzed by what? - ✔✔ANSW✔✔..RNA Polymerase, mostly in
interphase
RNA Pol. I and III - ✔✔ANSW✔✔..synthesize non-coding RNA
RNA Pol. II - ✔✔ANSW✔✔..responsible for synthesis of mRNA
Transcription Initiation - ✔✔ANSW✔✔..RNA Pol. and its supporting accessory proteins
assemble on DNA at a specific site called the promoter.
What stains to the minor groove of DNA duplex? - ✔✔ANSW✔✔..SYBR Green
What is the most abundant type of RNA? - ✔✔ANSW✔✔..Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - ✔✔ANSW✔✔..Genome and mRNA
What increases half-life of mRNA? - ✔✔ANSW✔✔..5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? -
✔✔ANSW✔✔..Euchromatin
What is the most common type of eukaryotic DNA? - ✔✔ANSW✔✔..Satellite DNA
What specimen has the most DNA according to molecular weight? -
✔✔ANSW✔✔..White Blood Cells
, What amino acids compose histones? - ✔✔ANSW✔✔..Lysine and Arginine
Amino acid that all proteins start with: - ✔✔ANSW✔✔..Methionine
What protects the DNA strand during replication? - ✔✔ANSW✔✔..Single-stranded
binding protein
Leading Strand - ✔✔ANSW✔✔..3' to 5' template
Lagging Strand - ✔✔ANSW✔✔..5' to 3' template
What DNA strand is being copied in transcription? - ✔✔ANSW✔✔..Antisense Strand
First step after newly formed mRNA is made? - ✔✔ANSW✔✔..Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? -
✔✔ANSW✔✔..Telomerase
What polymerase has repair activity? - ✔✔ANSW✔✔..Beta/Epsilon
What polymerase acts on leading strand? - ✔✔ANSW✔✔..Delta
What polymerase acts on lagging strand? - ✔✔ANSW✔✔..Alpha
Which polymerase is used for mitochondrial replication and repair? -
✔✔ANSW✔✔..Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - ✔✔ANSW✔✔..Topoisomerase
Which RNA polymerase is mainly for transcription? - ✔✔ANSW✔✔..RNA Pol II
Which polymerase is involved with mRNA synthesis? - ✔✔ANSW✔✔..RNA Pol II
What inactivates RNAses? - ✔✔ANSW✔✔..Diethyl pyrocarbonate (DEPC) or bake
glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? -
✔✔ANSW✔✔..Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity? - ✔✔ANSW✔✔..Restriction
Enzyme Type II
What is necessary to make cDNA? - ✔✔ANSW✔✔..Reverse transcriptase
QUESTIONS AND CORRECT VERIFIED ANSWERS
(DETAILED ANSWERS) ALREADY GRADED A+ 100%
GUARANTEED TO PASS CONCEPTS!!!
Predominant form of DNA - ✔✔ANSW✔✔..Right-handed B
DNA and histone interaction is due to what type of bond? - ✔✔ANSW✔✔..Ionic
DNA methyltransferases - ✔✔ANSW✔✔..Prokaryotic DNA: mostly methylated or
hemimethylated. Asa means to differentiate host DNA from non-host and to provide
resistance to restriction enzymes. Eukaryotic DNA: is methylated in specific regions.
DNA- binding proteins may limit accessibility or guide methyltransferases to specific
regions of the DNA
DNA methytransferases catalyze the addition of methyl groups to nitrogen bases,
usually adenines and cytosines in DNA strands.
How does genetic information move in the conjugation process? - ✔✔ANSW✔✔..From
F+ to F- bacteria. F+ has a fertility factor that not only carried the information from one
cell to another but also was responsible for establishing hte physical connection
between the mating bacteria.
F factor (fertility factor) - ✔✔ANSW✔✔..extrachromosomal circle of double-stranded
DNA or plasmid carrying the genes coding for construction of the mating bridge. One
copy of the F factor remains in the F+ Bacteria and the other is sent into the F- Bacteria.
Protease. and Ribonuclease - ✔✔ANSW✔✔..degrades protein and RNA
Different physical states of the plasmid DNA CAN BE RESOLVED BY distinct _____
characteristcs during gel electrophoresis - ✔✔ANSW✔✔..migration
,What was the first indication of the episomal (plasmid) nature of the resistance factor? -
✔✔ANSW✔✔..Multiple drug resistance in bacteria can be eliminated by treatment with
acridine dyes
RTF or R factors - ✔✔ANSW✔✔..In resistant bacteria, plasmids carrying the genes for
inactivation or circumvention of antiobiotic action were called resistance factors.
Gene Expression - ✔✔ANSW✔✔..Inorder for DNA info to be utilized, it must be
transcribed and then translated into protein
Which RNA carries the info in DNA to hte ribosomes where it is translated into a
protein? - ✔✔ANSW✔✔..mRNA
Transcription - ✔✔ANSW✔✔..the copying of one strand of DNA into RNA by a process
similar to that of DNA replication
Transcription is catalyzed by what? - ✔✔ANSW✔✔..RNA Polymerase, mostly in
interphase
RNA Pol. I and III - ✔✔ANSW✔✔..synthesize non-coding RNA
RNA Pol. II - ✔✔ANSW✔✔..responsible for synthesis of mRNA
Transcription Initiation - ✔✔ANSW✔✔..RNA Pol. and its supporting accessory proteins
assemble on DNA at a specific site called the promoter.
What stains to the minor groove of DNA duplex? - ✔✔ANSW✔✔..SYBR Green
What is the most abundant type of RNA? - ✔✔ANSW✔✔..Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - ✔✔ANSW✔✔..Genome and mRNA
What increases half-life of mRNA? - ✔✔ANSW✔✔..5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? -
✔✔ANSW✔✔..Euchromatin
What is the most common type of eukaryotic DNA? - ✔✔ANSW✔✔..Satellite DNA
What specimen has the most DNA according to molecular weight? -
✔✔ANSW✔✔..White Blood Cells
, What amino acids compose histones? - ✔✔ANSW✔✔..Lysine and Arginine
Amino acid that all proteins start with: - ✔✔ANSW✔✔..Methionine
What protects the DNA strand during replication? - ✔✔ANSW✔✔..Single-stranded
binding protein
Leading Strand - ✔✔ANSW✔✔..3' to 5' template
Lagging Strand - ✔✔ANSW✔✔..5' to 3' template
What DNA strand is being copied in transcription? - ✔✔ANSW✔✔..Antisense Strand
First step after newly formed mRNA is made? - ✔✔ANSW✔✔..Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? -
✔✔ANSW✔✔..Telomerase
What polymerase has repair activity? - ✔✔ANSW✔✔..Beta/Epsilon
What polymerase acts on leading strand? - ✔✔ANSW✔✔..Delta
What polymerase acts on lagging strand? - ✔✔ANSW✔✔..Alpha
Which polymerase is used for mitochondrial replication and repair? -
✔✔ANSW✔✔..Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - ✔✔ANSW✔✔..Topoisomerase
Which RNA polymerase is mainly for transcription? - ✔✔ANSW✔✔..RNA Pol II
Which polymerase is involved with mRNA synthesis? - ✔✔ANSW✔✔..RNA Pol II
What inactivates RNAses? - ✔✔ANSW✔✔..Diethyl pyrocarbonate (DEPC) or bake
glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? -
✔✔ANSW✔✔..Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity? - ✔✔ANSW✔✔..Restriction
Enzyme Type II
What is necessary to make cDNA? - ✔✔ANSW✔✔..Reverse transcriptase
