Blood Bank - Serologic and Molecular Testing Exam Questions and Answers 100%
A patient with multiple myeloma has the following reactions in the ABO typing:
Anti-A= w+
Anti-B = w+
Anti-A,B = w+
Auto control = w+
A1 Cells = 4+
B cells = 4+
What is probably causing these results?
A. Rouleaux
B. Subgroup of A
C. Patient has hypogammaglobulinemia
D. Patient has selective IgA deficiency - Answers A
An antibody screen utilizing polyethylene glycol (PEG) was performed. Reaction readings of this antibody
screen are analyzed after immediate spin and again at 37°C incubation with subsequent centrifugation.
How will the centrifugation of the tubes affect the patient's results?
A. Reactions will be enhanced
B. No effect will be seen
C. False positives may be seen
D. False negatives may be seen - Answers C
What must be true for the antiglobulin phase of the serologic crossmatch to be omitted (i.e., immediate
spin crossmatch is done)?
A. The antibody screen must be negative and there is not history of detection of unexpected antibodies.
B. The patient has not been transfused within the past 24 hours.
,C. The blood is needed for surgery.
D. There is a history of detection of unexpected antibodies but the current antibody screen is negative. -
Answers A
Which of the following accurately represents reverse typing?
A. Reverse typing is performed using known reagent antisera to detect ABO antigens on the patient's
red blood cells.
B. Reverse typing is performed using known reagent red blood cells to detect ABO antibodies in the
patient's serum or plasma.
C. Reverse typing is performed using known reagent screening cells to detect clinically significant
antibodies in the patient's serum or plasma.
D. Reverse typing is performed using known reagent antisera to phenotype donor red blood cells for
clinically significant antigens. - Answers B
A former patient had an anti-E four years ago, but her antibody panel is now negative. Since she now
needs blood for surgery, what should the blood bank do?
A. Get autologous blood from relatives.
B. Crossmatch E-negative units.
C. Give group O negative whole blood.
D. Give random compatible units. - Answers B
What is the maximum interval during which a recipient sample may be used for compatibility testing if
the patient has recently been transfused or was pregnant within the past 3 months?
A. 24 hours
B. 3 days
C. One week
D. Two weeks - Answers B
A patient transfused with two units of packed cells spiked a fever of 99.5oF and complained of chills five
days after transfusion. The direct antiglobulin test (DAT) was positive with anti-IgG, but negative with
, anti-C3d. Compatibility testing was performed on the pre- and post-transfusion specimens. The post-
transfusion specimen was incompatible with one of the donor units transfused. An antibody screen was
done on both the pre- and post-transfusion specimens. An antibody was detected in the post-
transfusion specimen only and identified by panel studies as anti-Jka. This transfusion reaction is most:
A. Post-transfusion purpura
B. An anaphylactic response
C. An acute hemolytic transfusion reaction
D. A delayed hemolytic transfusion reaction - Answers D
You perform a gel ABO/Rh test on a 45-year-old male patient and you obtain the following results.
Anti-A - 4+
Anti-B - 4+
Anti-D - 4+
D control - negative
A1 cells - 2+
B cells - 0
What could be the Type/Rh of this patient?
a. AB POS
b. A POS
c. B POS
d. A2B POS - Answers D
When performing an anti-human globulin (AHG) test, it is important to completely wash the red cells
because:
A patient with multiple myeloma has the following reactions in the ABO typing:
Anti-A= w+
Anti-B = w+
Anti-A,B = w+
Auto control = w+
A1 Cells = 4+
B cells = 4+
What is probably causing these results?
A. Rouleaux
B. Subgroup of A
C. Patient has hypogammaglobulinemia
D. Patient has selective IgA deficiency - Answers A
An antibody screen utilizing polyethylene glycol (PEG) was performed. Reaction readings of this antibody
screen are analyzed after immediate spin and again at 37°C incubation with subsequent centrifugation.
How will the centrifugation of the tubes affect the patient's results?
A. Reactions will be enhanced
B. No effect will be seen
C. False positives may be seen
D. False negatives may be seen - Answers C
What must be true for the antiglobulin phase of the serologic crossmatch to be omitted (i.e., immediate
spin crossmatch is done)?
A. The antibody screen must be negative and there is not history of detection of unexpected antibodies.
B. The patient has not been transfused within the past 24 hours.
,C. The blood is needed for surgery.
D. There is a history of detection of unexpected antibodies but the current antibody screen is negative. -
Answers A
Which of the following accurately represents reverse typing?
A. Reverse typing is performed using known reagent antisera to detect ABO antigens on the patient's
red blood cells.
B. Reverse typing is performed using known reagent red blood cells to detect ABO antibodies in the
patient's serum or plasma.
C. Reverse typing is performed using known reagent screening cells to detect clinically significant
antibodies in the patient's serum or plasma.
D. Reverse typing is performed using known reagent antisera to phenotype donor red blood cells for
clinically significant antigens. - Answers B
A former patient had an anti-E four years ago, but her antibody panel is now negative. Since she now
needs blood for surgery, what should the blood bank do?
A. Get autologous blood from relatives.
B. Crossmatch E-negative units.
C. Give group O negative whole blood.
D. Give random compatible units. - Answers B
What is the maximum interval during which a recipient sample may be used for compatibility testing if
the patient has recently been transfused or was pregnant within the past 3 months?
A. 24 hours
B. 3 days
C. One week
D. Two weeks - Answers B
A patient transfused with two units of packed cells spiked a fever of 99.5oF and complained of chills five
days after transfusion. The direct antiglobulin test (DAT) was positive with anti-IgG, but negative with
, anti-C3d. Compatibility testing was performed on the pre- and post-transfusion specimens. The post-
transfusion specimen was incompatible with one of the donor units transfused. An antibody screen was
done on both the pre- and post-transfusion specimens. An antibody was detected in the post-
transfusion specimen only and identified by panel studies as anti-Jka. This transfusion reaction is most:
A. Post-transfusion purpura
B. An anaphylactic response
C. An acute hemolytic transfusion reaction
D. A delayed hemolytic transfusion reaction - Answers D
You perform a gel ABO/Rh test on a 45-year-old male patient and you obtain the following results.
Anti-A - 4+
Anti-B - 4+
Anti-D - 4+
D control - negative
A1 cells - 2+
B cells - 0
What could be the Type/Rh of this patient?
a. AB POS
b. A POS
c. B POS
d. A2B POS - Answers D
When performing an anti-human globulin (AHG) test, it is important to completely wash the red cells
because:
