Exam Questions and Answers Advanced Blood Bank 100% Pass

Exam Questions and Answers Advanced Blood Bank 100% Pass DAT - Answers What antiglobulin test is a one step test to detect red cells sensitized with antibody or complement in vivo (in the body)? Indirect (test manipulated environment) - Answers What antiglobulin test is a two step method to determine antibody reacts with antigen in vitro? EDTA - Answers What specimen tube would you use to perform an antiglobulin test? chelates calcium and stops complement - Answers Why would you use an EDTA tube to perform an antiglobulin test? Coombs Control check cells - Answers What reagent helps tell you that AHG was added and it was active and the washing was adequate in the antiglobulin test? LISS - Answers What potentiator reduces ionic strength so agglutination between antigen and antibody uptakes at a faster rate? PEG - Answers What potentiator removes water molecules in the test environment to allow for antigen/antibody attraction? Rh, Kidd, Lewis, P1, I - Answers Proteolytic enzymes enhances these antigens? Duffy, MNS, Xga - Answers Proteolytic enzymes destroys these enzymes? Monoclonal-Polyclonal Blend - Answers What D reagent is used more often that is a hybridoma produced with IgM blended with human polyclonal IgG? High Protein Anti-D - Answers What D reagent is highly viscous and enhances agglutination and must use a Rh negative control with it? ensures test was not false negative (test was valid) - Answers What does check cells do for us? A1 - Answers What red blood cell antigen does the lectin Dolichos biflorus bind to? H - Answers What red blood cell antigen does the lectin Ulex europaeus bind to? N - Answers What red blood cell antigen does the lectin Vicia graminea bind to? M - Answers What red blood cell antigen does the lectin Iberis amara bind to? contamination, expired, storage conditions - Answers The quality control for Anti-D reagent has started to decrease in potency, what could cause this to happen? IAT (ab screen, ab id, xm, antigen typing) - Answers What antiglobulin test is the most versatile? Monospecific - Answers What reagent uses complement or IgG singly and is used for multiple tests? Polyspecific - Answers What reagent contains IgG and complement and is used for DAT? No agglutination (indicating that the test isn't valid and something was forgotten) - Answers The tech was interrupted in the AHG test when the phone rang. He forgot to add the AHG reagent and when he added the check cells what would happen? negative - Answers You have added IgG sensitized red cells to the negative IAT test result, you observe agglutination in the tube. Is the screen positive or negative? Lectins - Answers Reagent that is derived from plants used to distinguish group A1 from group A2 red cells? ABO antisera - Answers Reagent used to determine the ABO antigenic composition of a patient's red cells? screen cells - Answers Reagent to detect the presence of red cell antibodies? Panel cells - Answers Reagent to identify the specificity of a red cell antibody? A1/B cells - Answers Reagent used in the identification of the ABO antibodies? hemolysis - Answers What can happen to a PRBC unit if it goes below the temperature of 1 degree Cel? bacteria growth - Answers What can happen to a PRBC unit if it goes above 4 deg Cel? Proficiency test - Answers The CAP survey is an example of this? Audit - Answers Systematic evaluations to determine whether procedures are being followed? QC - Answers Testing to determine the accuracy and precision of reagents and equipment? calibration - Answers Process of standardizing an instrument against a known value? Recall - Answers Removal of products from the market that might compromise the safety of the recipient? accuracy - Answers Degree of measurement represents the true value? validation - Answers Establishing that a specific process produces an expected result? competency (assessment) - Answers Evaluation of an employee's ability to perform a specific skill? Root cause (analysis) - Answers Investigation and identification of the factors that contributed to an error? preventive maintenance - Answers Maximizes the duration of equipment and increases the reliability of the equipment? change control - Answers The System to plan and implement changes to prevent problems? A3 - Answers What subgroup has a mixed field pattern with Anti-A from group O or B donors? Ax and Ael - Answers What 2 subgroup has weakened reactivity with one being the weakest? Cis AB (phenotype) - Answers What is the term is a rare chromosome encodes for an enzyme with A and B transferees on the same gene? Cis AB (phenotype) - Answers What discrepancy could be considered if you have a AB mother with an AB baby, but the father is O? A or B antigens weak or m/f - Answers What can happen to reverse type of patients with Leukemia? Chimera - Answers What is the term for mixed field reactivity that can happen from transfusion or transplant, in-utero exchange, or dispermy? Acquired B - Answers What is the discrepancy of a A1 person that can acquire a B-like antigen that is presumed to be caused by action of gram-negative bacteria? Acquired B - Answers A patient forward types as a AB and their reverse type makes an Anti-B, what could be the subgroup discrepancy? B(A) (phenomenon) - Answers What is the discrepancy when cells react weakly to anti-A1, but strongly with anti-B? hyaluronidase (from chemistry dept) - Answers What would you use to disperse Wharton's jelly in contaminated cord blood specimens? rouleaux - Answers What could be the problem from extra reactivity due to elevated protein levels from fibrinogen? same (+o) - Answers What type of alleles does homozygous have? different (++) - Answers What type of alleles does heterozygous have? stronger homozygous - Answers Anti M can show dosage which means it can show what kind of agglutination? not hetero or homo (they are built) - Answers Why does Lewis antibodies don't express dosage? lack both A and B antigens (won't react with naturally occurring antibodies) - Answers Why does ABO type antibody screen and antibody panel cells use type O?