ASCP PRACTICE (MB) EXAM QUESTION AND CORRECT
ANSWER (2024-2025) 100% COMPLETE PASS
Question 1
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base
Anti-codon
Question 2
According to Chargaff's rule of base pairing, adenine pairs with:
Thymine
Question 3
What genes would be screened in a breast cancer panel?
HER2, ERBB2, BRCA1
Question 4
Next Generation Sequencing uses:
Short sequence reads
ASCP PRACTICE (MB) EXAM
,Question 5
Purines and pyrimidines differ from each other in that:
Purines have two rings; pyrimidines have one ring
Question 6
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine
Adenine and guanine
Question 7
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs
1 in 1,000,000,000 base pairs
Question 8
The rate of DNA migration through an agarose gel during electrophoresis does not depend on which
of the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule
Nucleotide sequence of the molecule
ASCP PRACTICE (MB) EXAM
,Question 9
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau
Initiation, exponential, plateau
Question 10
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC
3'-GAC
Question 11
A patient with impaired judgment, personality changes, signs of abnormal body movements and
depression comes to the physician's office for a follow-up visit. The physician suspects a single-gene
disorder may be the cause of those clinical manifestations. A blood specimen was then sent to your
clinical laboratory for mutation screening in the Huntington gene. Testing with standard PCR indicates
that patient has Huntington Disease, HD. Which of the following would be consistent with this
diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene
85 CAG repeats in the Huntington gene
ASCP PRACTICE (MB) EXAM
, Question 12
Which two HPV types are responsible for most cases of cervical cancer?
16 and 18
31 and 59
16 and 58
44 and 59
16 and 18
Question 13
Replication forks, known as origins of DNA replication, are created by this enzyme:
Ligase
Taq Polymerase
Primase
Helicase
Helicase
Question 14
Mutation in what gene is associated with Fragile X syndrome?
FMR1
Question 15
Mantle cell lymphoma (MCL) is caused by what translocation?
t(11;14)
Question 16
This polymerase is involved in "initiation of DNA replication and has primase activity":
Pol α
ASCP PRACTICE (MB) EXAM
ANSWER (2024-2025) 100% COMPLETE PASS
Question 1
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base
Anti-codon
Question 2
According to Chargaff's rule of base pairing, adenine pairs with:
Thymine
Question 3
What genes would be screened in a breast cancer panel?
HER2, ERBB2, BRCA1
Question 4
Next Generation Sequencing uses:
Short sequence reads
ASCP PRACTICE (MB) EXAM
,Question 5
Purines and pyrimidines differ from each other in that:
Purines have two rings; pyrimidines have one ring
Question 6
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine
Adenine and guanine
Question 7
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs
1 in 1,000,000,000 base pairs
Question 8
The rate of DNA migration through an agarose gel during electrophoresis does not depend on which
of the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule
Nucleotide sequence of the molecule
ASCP PRACTICE (MB) EXAM
,Question 9
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau
Initiation, exponential, plateau
Question 10
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC
3'-GAC
Question 11
A patient with impaired judgment, personality changes, signs of abnormal body movements and
depression comes to the physician's office for a follow-up visit. The physician suspects a single-gene
disorder may be the cause of those clinical manifestations. A blood specimen was then sent to your
clinical laboratory for mutation screening in the Huntington gene. Testing with standard PCR indicates
that patient has Huntington Disease, HD. Which of the following would be consistent with this
diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene
85 CAG repeats in the Huntington gene
ASCP PRACTICE (MB) EXAM
, Question 12
Which two HPV types are responsible for most cases of cervical cancer?
16 and 18
31 and 59
16 and 58
44 and 59
16 and 18
Question 13
Replication forks, known as origins of DNA replication, are created by this enzyme:
Ligase
Taq Polymerase
Primase
Helicase
Helicase
Question 14
Mutation in what gene is associated with Fragile X syndrome?
FMR1
Question 15
Mantle cell lymphoma (MCL) is caused by what translocation?
t(11;14)
Question 16
This polymerase is involved in "initiation of DNA replication and has primase activity":
Pol α
ASCP PRACTICE (MB) EXAM
