BIO 111 LAB TAMU EXAM 1 WITH
QUESTIONS AND WELL VERIFIED ANSWERS
[ALREADY GRADED A+} REAL EXAM !!!
Molar weight - ANS✔✔---a measure of mass. By definition, a mole is 6x1023 objects
Standard curve - ANS✔✔---a graph in which properties of known samples are
measured and plotted. The direct proportionality of these properties can then be used to
determine the same information from unknown samples. In this lab, a standard curve of
Absorbance versus protein concentration will be used to determine the concentration of
protein in an unknown sample.
Concentration - ANS✔✔---C1V1=C2V2
Enzyme - ANS✔✔---molecules which catalyze biochemical reaction and bind specific
reactants, referred to as substrates.
Enzyme activity - ANS✔✔---is affected by the concentration of both enzyme and
substrates, temp, and pH.
, β-Galactosidase (β-Gal) - ANS✔✔---an enzyme produced by Escherichia coli (E. coli)
that catalyzes the hydrolysis (breakdown of a molecule by using up a water molecule) of the
disaccharide lactose to the monosaccharides galactose and glucose
ONPG and galactose - ANS✔✔---are colorless
Ortho-nitrophenol - ANS✔✔---is bright yellow with an absorption wavelength of 420 nm.
Because of the colored product, we can measure enzyme activity by monitoring ortho-
nitrophenol production using a spectrophotometer.
Cellular respiration - ANS✔✔---all the processes a living organism uses to produce ATP
(adenosine triphosphate). These processes include: glycolysis, fermentation, oxidation of
pyruvate, and the Krebs cycle
Cellular respiration 2 - ANS✔✔---cellular respiration will use O2 as a final electron
acceptor. This aerobic respiration results in a consumption of O2 as the cells produce ATP.
Anaerobic respiration (fermentation) - ANS✔✔---generally produces fewer ATP
molecules (through glycolysis), but is useful when O2 is not available as the final electron
acceptor. Instead, an organic molecule (or sometimes an inorganic compound) is used as
the final electron acceptor, resulting in oxidation of NADH back to NAD+ at the end of the
reactions. In the absence of O2, the NAD+ must be continually regenerated through these
reactions in order to continue to produce ATP through the glycolytic pathway
Accuracy - ANS✔✔---a measure of how close a result matches a correct or standard
value
QUESTIONS AND WELL VERIFIED ANSWERS
[ALREADY GRADED A+} REAL EXAM !!!
Molar weight - ANS✔✔---a measure of mass. By definition, a mole is 6x1023 objects
Standard curve - ANS✔✔---a graph in which properties of known samples are
measured and plotted. The direct proportionality of these properties can then be used to
determine the same information from unknown samples. In this lab, a standard curve of
Absorbance versus protein concentration will be used to determine the concentration of
protein in an unknown sample.
Concentration - ANS✔✔---C1V1=C2V2
Enzyme - ANS✔✔---molecules which catalyze biochemical reaction and bind specific
reactants, referred to as substrates.
Enzyme activity - ANS✔✔---is affected by the concentration of both enzyme and
substrates, temp, and pH.
, β-Galactosidase (β-Gal) - ANS✔✔---an enzyme produced by Escherichia coli (E. coli)
that catalyzes the hydrolysis (breakdown of a molecule by using up a water molecule) of the
disaccharide lactose to the monosaccharides galactose and glucose
ONPG and galactose - ANS✔✔---are colorless
Ortho-nitrophenol - ANS✔✔---is bright yellow with an absorption wavelength of 420 nm.
Because of the colored product, we can measure enzyme activity by monitoring ortho-
nitrophenol production using a spectrophotometer.
Cellular respiration - ANS✔✔---all the processes a living organism uses to produce ATP
(adenosine triphosphate). These processes include: glycolysis, fermentation, oxidation of
pyruvate, and the Krebs cycle
Cellular respiration 2 - ANS✔✔---cellular respiration will use O2 as a final electron
acceptor. This aerobic respiration results in a consumption of O2 as the cells produce ATP.
Anaerobic respiration (fermentation) - ANS✔✔---generally produces fewer ATP
molecules (through glycolysis), but is useful when O2 is not available as the final electron
acceptor. Instead, an organic molecule (or sometimes an inorganic compound) is used as
the final electron acceptor, resulting in oxidation of NADH back to NAD+ at the end of the
reactions. In the absence of O2, the NAD+ must be continually regenerated through these
reactions in order to continue to produce ATP through the glycolytic pathway
Accuracy - ANS✔✔---a measure of how close a result matches a correct or standard
value
