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Exam (elaborations)

Exam (elaborations) CHL2601

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ENTAILS QUESTIONS AND ANSWERS

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CHL2601
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September 21, 2024
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CHL2601 Assignment 8 (COMPLETE ANSWERS) 2024
(165437) - DUE 23 September 2024

,Which of the following shows the moist heat methods in order of the temperature used
(highest to lowest)?

Autoclave>Ultrahigh>Batch Pasteurization>Boiling
Ultrahigh>Boiling>Autoclave>Batch Pasteurization
Ultrahigh> Boiling>Batch Pasteurization >Autoclave
Ultrahigh>Autoclave>Boiling>Batch Pasteurization - CORRECT ANSWERS-
Ultrahigh>Autoclave>Boiling>Batch Pasteurization

_______________ is a process used to destroy pathogens in food and drinks. It must
be done at a lower temperature than other methods so that _______________. -
CORRECT ANSWERS-Pasteurization; the taste of the food or drink will not be altered

During a flood, a community's well water system is contaminated with a pathogenic
fungus. If the residents boil their water for 15 minutes prior to consumption, will they be
protected? - CORRECT ANSWERS-yes; most likely

During production of a drug, all work area surfaces must be disinfected using sterilized
disinfectants. Which of the following statements about disinfectants are true?
Select all that apply.

Disinfectants destroy all microbes present on a surface.
Disinfectants are used to inhibit or destroy pathogens.
Disinfection can occur by physical or chemical means.
Disinfection can be used to treat work surfaces as well as workers (i.e., washing
hands).
Endospores and viruses can resist disinfectant treatment. - CORRECT ANSWERS-
Disinfectants are used to inhibit or destroy pathogens.
Disinfection can occur by physical or chemical means.
Endospores and viruses can resist disinfectant treatment.

Assume that you have an antimicrobial agent specific for each of the targets listed
below. Indicate which type of microbe would be most susceptible to the agent by placing
it in the appropriate bin. - CORRECT ANSWERS-All bacteria:
-membrane proteins
-glycolytic enzymes

Gram-positive Bacteria:
-peptidoglycan

Viruses:
-envelope proteins

All bact and viruses:
-nucleic acids

, Apply your knowledge of the structural features of microbes and the mechanisms of
action for antimicrobial agents to rank the following microbes according to their
susceptibility. - CORRECT ANSWERS-Least susceptible
-prions
-endospores
-gram-negative bacteria
-gram-positive bacteria
-enveloped virus
Most susceptible

Choose the statement about autoclaving that is true.

The effectiveness of an autoclave is dependent on an increase in pressure and
corresponding increase in temperature.
Autoclaving requires extensive exposure times in order to achieve sterilization.
When autoclaving solid items, they should be wrapped in foil to help maintain sterility.
Autoclaving is preferred over dry heat because it can effectively be used to sterilize a
wide variety of items, including liquids, powders, metal instruments, and glassware. -
CORRECT ANSWERS-The effectiveness of an autoclave is dependent on an increase
in pressure and corresponding increase in temperature.

One alternative to autoclaving for sterilization of liquids is filtration. Imagine that you are
using a filter to sterilize a drug solution. You use a filter with a 0.22 μm pore size. Which
of the following microbes will effectively be removed by this filter?
Select all that apply.

fungi
bacteria such as E. coli
small viruses
yeast - CORRECT ANSWERS-fungi
bacteria such as E. coli
yeast

It has been determined that the temperature in an autoclave should reach __________
for sterilization. - CORRECT ANSWERS-121 degrees Celsius

What role does pressure play in an autoclave? - CORRECT ANSWERS-Pressure is
applied to boiling water to prevent heat from escaping as steam.

How do scientists ensure that autoclaved materials were actually sterilized? -
CORRECT ANSWERS-They may place a sterility indicator with a special dye to detect
the growth of endospores

What is the purpose of an autoclave?

to incubate cultures
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