ASCP Recall study questions with correct
answers.
Procedure #1 detected 50/100 true positives & 100/100 true negatives. Procedure #2 detected 80/100
true positives and 70/100 true negatives. - Correct Answer Procedure 2 is more sensitive (refers to % of
true positives that are accurately identified as positives). Specificity refers to # of true negatives
identified as negative.
TP/TP+FN= - Correct Answer Sensitivity
TN/TN+FP= - Correct Answer Specificity
What might the following indicate in urine: RBCs, WBCs, nitrite, and bacteria? - Correct Answer
Pyelonephritis (UTI); WBCs and nitrites in urine are classical indication of bacterial infection (UTI)
Why is albumin the first protein to be detected in tests for renal failure? - Correct Answer It's molecular
size is the smallest
Cortisol excess will result in: - Correct Answer Elevated glucose in blood; cortisol counteracts insulin
What is the reason for the following discrepency?
Anti A: 3+
Anti B: 3+
A1: 3+
B cells: 0 - Correct Answer Patient may be A2B
This spiral form organism is seen in urine and cultured on fletcher's medium - Correct Answer Leptospira
Organism that gives off a bleach-like odor in culture: - Correct Answer Eikenella (GN pits agar)
,Presence of Rheumatoid factor in blood may result in false positives for what test? - Correct Answer
VDRL; nonspecific screening test for syphillis
Disease associated with the following: increased TSH increased T3 and increased FT4: - Correct Answer
Pituitary tumor
If excess PTH is released, what woulde you find elevated amounts of in serum? - Correct Answer Calcium
Mucoid pink colonies on plate that produce gase, indole positive, yellow/yellow on TSI - Correct Answer
Klebsiella oxytoca (Klebsiella pneumo is indole negative)
PAD positive, indole positive, organism stains gram negative. What is it? - Correct Answer Proteus
vulgaris (p. mirabilis is indole negative)
Curved gram negative bacilli. Cultured form GI tract of persons with ulcers. What test would you do to
confirm its identity? - Correct Answer Test for Urease. Most likely H. pylori which is curved, infects GI
tract, and the urease activity can cause ulcers.
Enzyme controls run on a machine gives result around -3SD. Samples run on the same machine give
results <1 SD. What is the problem? - Correct Answer Controls were left @ room temperature for
several days.
HIV-1 and HIV-2 combination ELISA test is positive in a patient with symptoms of immune deficiency.
Western blot was inconclusive for HIV-1. What do you do next? - Correct Answer DO HIV-2 western blot.
What are steps of PCR? - Correct Answer Denaturation, annealing, and transcription.
RAST test detects what? - Correct Answer IgE to particular antigens (RIST is for total IgE)
After collecting a blood sample in an EDTA tube for CBC you find that the Hct is very high (68%). What
should you do next? - Correct Answer Report results. Always use EDTA for CBC specimens.
, When you conduct a procedure using fluorescence it is important to protect yourself from: - Correct
Answer Exciting light; high energy light produced by the machine
Blood was collected Nov. 1st then frozen with glycol on Nov. 5th. What is the new expiration date? -
Correct Answer Nov. 1 10 years from now.
A person was successfully treated for syphillis 12 years ago. he just came in worried about being
reinfected. what would you look for in his blood? - Correct Answer VDRL; TPA is not correct because it
can remain active for the life of the patient.
Patient demonstrates positive antibody screen. You suspect either Jk(a), K or c. You know from previous
history that the patient has Jk(a) antigen on their red cells. You react the patients serum with cells
positive for certain antigens and see: K cells: 0, c cells: 4+. What can be concluded - Correct Answer You
can rule out c antigen but cannot confirm absence or presence of K antigen on patients red cells. Patient
producing anti-c and therefore does not have the c antigen. K did not react so patient either has K
antigen or has never been exposed to K and therefore does not make antibodies to K.
Urine protein chemistry dipstick detected no proteins by sulfosalicylic acid (SSA) test did detect proteins.
Why? - Correct Answer Bence-jones proteins in urine
Several immature granulocytes in PBS. What stain should be used to figure this persons problem out? -
Correct Answer LAP
Syndrome of inappropriate antidiuretic hormone secretions (SIADH) would result in what in blood? -
Correct Answer Deficient in sodium
Fiber strands in urine resemble what under the microscope? - Correct Answer Hyaline casts
Which of the following regulates myocyte contraction - Correct Answer Cardiac troponins
HBA1C levels cannot always be used to monitor glucose levels in conditions such as: - Correct Answer
Sickle cell disease; any disorder that causes RBCs to die prematurely (sickle cell, g6pd etc) will result in
underestimation of HBA1C levels
answers.
Procedure #1 detected 50/100 true positives & 100/100 true negatives. Procedure #2 detected 80/100
true positives and 70/100 true negatives. - Correct Answer Procedure 2 is more sensitive (refers to % of
true positives that are accurately identified as positives). Specificity refers to # of true negatives
identified as negative.
TP/TP+FN= - Correct Answer Sensitivity
TN/TN+FP= - Correct Answer Specificity
What might the following indicate in urine: RBCs, WBCs, nitrite, and bacteria? - Correct Answer
Pyelonephritis (UTI); WBCs and nitrites in urine are classical indication of bacterial infection (UTI)
Why is albumin the first protein to be detected in tests for renal failure? - Correct Answer It's molecular
size is the smallest
Cortisol excess will result in: - Correct Answer Elevated glucose in blood; cortisol counteracts insulin
What is the reason for the following discrepency?
Anti A: 3+
Anti B: 3+
A1: 3+
B cells: 0 - Correct Answer Patient may be A2B
This spiral form organism is seen in urine and cultured on fletcher's medium - Correct Answer Leptospira
Organism that gives off a bleach-like odor in culture: - Correct Answer Eikenella (GN pits agar)
,Presence of Rheumatoid factor in blood may result in false positives for what test? - Correct Answer
VDRL; nonspecific screening test for syphillis
Disease associated with the following: increased TSH increased T3 and increased FT4: - Correct Answer
Pituitary tumor
If excess PTH is released, what woulde you find elevated amounts of in serum? - Correct Answer Calcium
Mucoid pink colonies on plate that produce gase, indole positive, yellow/yellow on TSI - Correct Answer
Klebsiella oxytoca (Klebsiella pneumo is indole negative)
PAD positive, indole positive, organism stains gram negative. What is it? - Correct Answer Proteus
vulgaris (p. mirabilis is indole negative)
Curved gram negative bacilli. Cultured form GI tract of persons with ulcers. What test would you do to
confirm its identity? - Correct Answer Test for Urease. Most likely H. pylori which is curved, infects GI
tract, and the urease activity can cause ulcers.
Enzyme controls run on a machine gives result around -3SD. Samples run on the same machine give
results <1 SD. What is the problem? - Correct Answer Controls were left @ room temperature for
several days.
HIV-1 and HIV-2 combination ELISA test is positive in a patient with symptoms of immune deficiency.
Western blot was inconclusive for HIV-1. What do you do next? - Correct Answer DO HIV-2 western blot.
What are steps of PCR? - Correct Answer Denaturation, annealing, and transcription.
RAST test detects what? - Correct Answer IgE to particular antigens (RIST is for total IgE)
After collecting a blood sample in an EDTA tube for CBC you find that the Hct is very high (68%). What
should you do next? - Correct Answer Report results. Always use EDTA for CBC specimens.
, When you conduct a procedure using fluorescence it is important to protect yourself from: - Correct
Answer Exciting light; high energy light produced by the machine
Blood was collected Nov. 1st then frozen with glycol on Nov. 5th. What is the new expiration date? -
Correct Answer Nov. 1 10 years from now.
A person was successfully treated for syphillis 12 years ago. he just came in worried about being
reinfected. what would you look for in his blood? - Correct Answer VDRL; TPA is not correct because it
can remain active for the life of the patient.
Patient demonstrates positive antibody screen. You suspect either Jk(a), K or c. You know from previous
history that the patient has Jk(a) antigen on their red cells. You react the patients serum with cells
positive for certain antigens and see: K cells: 0, c cells: 4+. What can be concluded - Correct Answer You
can rule out c antigen but cannot confirm absence or presence of K antigen on patients red cells. Patient
producing anti-c and therefore does not have the c antigen. K did not react so patient either has K
antigen or has never been exposed to K and therefore does not make antibodies to K.
Urine protein chemistry dipstick detected no proteins by sulfosalicylic acid (SSA) test did detect proteins.
Why? - Correct Answer Bence-jones proteins in urine
Several immature granulocytes in PBS. What stain should be used to figure this persons problem out? -
Correct Answer LAP
Syndrome of inappropriate antidiuretic hormone secretions (SIADH) would result in what in blood? -
Correct Answer Deficient in sodium
Fiber strands in urine resemble what under the microscope? - Correct Answer Hyaline casts
Which of the following regulates myocyte contraction - Correct Answer Cardiac troponins
HBA1C levels cannot always be used to monitor glucose levels in conditions such as: - Correct Answer
Sickle cell disease; any disorder that causes RBCs to die prematurely (sickle cell, g6pd etc) will result in
underestimation of HBA1C levels
