MIP 302 - Exam 1 2024 with Answers to
Questions
How do molds reproduce? - ✔✔Yeasts reproduce asexually by budding and sexually
through the production of spores; molds and mushrooms reproduce asexually through
spores and sexually through gamete and spore formation
What do yeasts produce under aerobic conditions? - ✔✔Carbon dioxide
What do yeasts produce under anaerobic conditions? - ✔✔Carbon dioxide and
alcohol through fermentation
What are hyphae? - ✔✔Long, threadlike filaments that join together to form the
mycelium
What are hyphae called when protoplasms stream continuously through them? -
✔✔Coenocytic hyphae
What are hyphae called when they have cross walls between them? - ✔✔Septate
hyphae
What are the different variations of hyphae? - ✔✔Coenocytic hyphae and septate
hyphae
What are mycelium? - ✔✔A network of hyphae
,What are sporangium? - ✔✔Aerial hyphae that produce spores; give molds their
'hairy' appearance
What are spores? - ✔✔a minute, typically one-celled, reproductive unit capable of
giving rise to a new individual without sexual fusion
When preparing a slide for staining, why is it important to only place a small amount of
cells on the slide? - ✔✔So as not to saturate the slide while viewing it
Why are slides allowed to dry completely before heat-fixing? - ✔✔So that we don't
boil the microbes and cause them to pop
How does one determine whether one's cells are algae or protozoans? - ✔✔Algae are
photosynthetic so they will appear green or yellow; protozoa are usually motile
What is the function of protozoa in rumen fluid? - ✔✔Producing the enzymes that
degrade cellulose which allows the host to use it as an energy source
How do you calculate total magnification? - ✔✔Total Magnification = Objective
Magnification x Ocular Magnification
How do you calculate the resolving power of a microscope? - ✔✔Resolving Power =
(0.5 x Wavelength)/Numerical Aperture
What does resolving power mean? - ✔✔The microscope's ability to distinguish
between two objects that are very close together
What does numerical aperture mean? - ✔✔A lens' ability to gather light
, Would a microscope with a resolving power of 0.42 um or 0.2um be better for viewing
bacteria? Why? - ✔✔0.2um because it would have a lower resolving power meaning
you would better be able to distinguish between two bacteria that are very close
together
What is oil immersion? - ✔✔100x objective magnification using a drop of oil
Why do we use oil immersion? - ✔✔To get a better resolving power for viewing
organisms
Why is oil immersion only used with the 100x objective lens? - ✔✔To minimize
refraction and maximize the amount of light entering the lens
What is the difference between tryptic soy agar and tryptic soy broth? - ✔✔TSB is a
broth so it is liquid while TSA is an agar so it is solid; TSB allows you to determine if
bacteria are present but no other information; TSA allows you to isolate the bacteria
What kind of media is tryptic soy? - ✔✔All purpose medium that supports the growth
of bacteria that do not have special nutritional needs
What is turbidity? - ✔✔How cloudy a broth is
Why are broths and slants better suited as culture media as opposed to isolation media?
- ✔✔They allow you to see that bacteria have grown but provides no information as
to the types of bacteria
Why are agar plates well suited for bacterial isolation? - ✔✔They allow you to isolate
the bacteria and view the types of bacteria present
Questions
How do molds reproduce? - ✔✔Yeasts reproduce asexually by budding and sexually
through the production of spores; molds and mushrooms reproduce asexually through
spores and sexually through gamete and spore formation
What do yeasts produce under aerobic conditions? - ✔✔Carbon dioxide
What do yeasts produce under anaerobic conditions? - ✔✔Carbon dioxide and
alcohol through fermentation
What are hyphae? - ✔✔Long, threadlike filaments that join together to form the
mycelium
What are hyphae called when protoplasms stream continuously through them? -
✔✔Coenocytic hyphae
What are hyphae called when they have cross walls between them? - ✔✔Septate
hyphae
What are the different variations of hyphae? - ✔✔Coenocytic hyphae and septate
hyphae
What are mycelium? - ✔✔A network of hyphae
,What are sporangium? - ✔✔Aerial hyphae that produce spores; give molds their
'hairy' appearance
What are spores? - ✔✔a minute, typically one-celled, reproductive unit capable of
giving rise to a new individual without sexual fusion
When preparing a slide for staining, why is it important to only place a small amount of
cells on the slide? - ✔✔So as not to saturate the slide while viewing it
Why are slides allowed to dry completely before heat-fixing? - ✔✔So that we don't
boil the microbes and cause them to pop
How does one determine whether one's cells are algae or protozoans? - ✔✔Algae are
photosynthetic so they will appear green or yellow; protozoa are usually motile
What is the function of protozoa in rumen fluid? - ✔✔Producing the enzymes that
degrade cellulose which allows the host to use it as an energy source
How do you calculate total magnification? - ✔✔Total Magnification = Objective
Magnification x Ocular Magnification
How do you calculate the resolving power of a microscope? - ✔✔Resolving Power =
(0.5 x Wavelength)/Numerical Aperture
What does resolving power mean? - ✔✔The microscope's ability to distinguish
between two objects that are very close together
What does numerical aperture mean? - ✔✔A lens' ability to gather light
, Would a microscope with a resolving power of 0.42 um or 0.2um be better for viewing
bacteria? Why? - ✔✔0.2um because it would have a lower resolving power meaning
you would better be able to distinguish between two bacteria that are very close
together
What is oil immersion? - ✔✔100x objective magnification using a drop of oil
Why do we use oil immersion? - ✔✔To get a better resolving power for viewing
organisms
Why is oil immersion only used with the 100x objective lens? - ✔✔To minimize
refraction and maximize the amount of light entering the lens
What is the difference between tryptic soy agar and tryptic soy broth? - ✔✔TSB is a
broth so it is liquid while TSA is an agar so it is solid; TSB allows you to determine if
bacteria are present but no other information; TSA allows you to isolate the bacteria
What kind of media is tryptic soy? - ✔✔All purpose medium that supports the growth
of bacteria that do not have special nutritional needs
What is turbidity? - ✔✔How cloudy a broth is
Why are broths and slants better suited as culture media as opposed to isolation media?
- ✔✔They allow you to see that bacteria have grown but provides no information as
to the types of bacteria
Why are agar plates well suited for bacterial isolation? - ✔✔They allow you to isolate
the bacteria and view the types of bacteria present
