PRACTICAL: MCB 2610 Uconn Dr. Rossi; Questions and
Answers 100% Solved
What is the process of contamination control? CORRECT ANS-Aseptic technique
What are pipettes CORRECT ANS-calibrated glass tubes used to measure and dispense
small volumes of liquids
What color is the bigger vs. smaller micropipettors pipettes? CORRECT ANS-Green - bigger
Blue - smaller
What is the purpose of isolating bacteria? CORRECT ANS-To establish a PURE CULTURE, to
be able to study to study the physiology and morphology of a particular bacterial type
What is the quickest and most economical method of bacterial isolation? CORRECT ANS-
Streak plate method
What must you use to view items under 100x? CORRECT ANS-immersion oil
, PRACTICAL: MCB 2610 Uconn Dr. Rossi; Questions and
Answers 100% Solved
What does parfocal mean? CORRECT ANS-It means that if you focus the specimen in one
objective, it'll be in approximate focus in all objectives
What is a simple stain? CORRECT ANS-Staining procedures that only use one stain
What is a direct stain? CORRECT ANS-A simple stain that colors the bacteria
What is a negative stain? CORRECT ANS-Simple stain that stains the background but leaves
the bacteria unstained
What is the purpose of heat fixing? CORRECT ANS-1. Denaturing bacterial enzymes to
prevent autolysis
2. Enhances the adherence of bacterial cells to microscope slide
In Gram stains, why can't Gram negative bacteria retain the crystal violet? CORRECT ANS-
Because Gram negative bacteria have an outer membrane and a thinner layer of
peptidoglycan, which are easily depolarized and can only retain the counterstain (Safranin-
pink)
Answers 100% Solved
What is the process of contamination control? CORRECT ANS-Aseptic technique
What are pipettes CORRECT ANS-calibrated glass tubes used to measure and dispense
small volumes of liquids
What color is the bigger vs. smaller micropipettors pipettes? CORRECT ANS-Green - bigger
Blue - smaller
What is the purpose of isolating bacteria? CORRECT ANS-To establish a PURE CULTURE, to
be able to study to study the physiology and morphology of a particular bacterial type
What is the quickest and most economical method of bacterial isolation? CORRECT ANS-
Streak plate method
What must you use to view items under 100x? CORRECT ANS-immersion oil
, PRACTICAL: MCB 2610 Uconn Dr. Rossi; Questions and
Answers 100% Solved
What does parfocal mean? CORRECT ANS-It means that if you focus the specimen in one
objective, it'll be in approximate focus in all objectives
What is a simple stain? CORRECT ANS-Staining procedures that only use one stain
What is a direct stain? CORRECT ANS-A simple stain that colors the bacteria
What is a negative stain? CORRECT ANS-Simple stain that stains the background but leaves
the bacteria unstained
What is the purpose of heat fixing? CORRECT ANS-1. Denaturing bacterial enzymes to
prevent autolysis
2. Enhances the adherence of bacterial cells to microscope slide
In Gram stains, why can't Gram negative bacteria retain the crystal violet? CORRECT ANS-
Because Gram negative bacteria have an outer membrane and a thinner layer of
peptidoglycan, which are easily depolarized and can only retain the counterstain (Safranin-
pink)
