©SOPHIEBENNETT
2024/2025 ACADEMIC YEAR
©SOPHIEBENNETT 9/1/24 2024/2025
,Micro Certification Exam Practice
Question with 100% Correct Answers
Which bacterial species is most likely represented by the spreading, wrinkled, non-
hemolytic colony appearance? - Answer✔️✔️-Burkholderia pseudomallei
Which one of the following tests should be used to monitor a patient's response to
treatment for syphilis? - Answer✔️✔️-RPR titer
A CSF specimen was sent to the laboratory for analysis. A glucose, protein, and cell
count were performed. Based on the following results, what would be the probable
cause?
Glucose -- 50 mg/dL
Protein -- 25 mg/dL
Leukocytes -- 2 cells/mm - Answer✔️✔️-Normal CSF
When performing quantitative cultures from bronchoalveolar lavage specimens, which
of the following colony forming units per millimeter indicates a true bacterial
pneumonia? - Answer✔️✔️->10 ^4 colony forming units/mL
The bacterium in the image to the right is also referred to in the quote below, what is the
identification on this bacteria?
"If a pregnant woman be attacked by erysipelas of the womb, it is fatal." - Hippocrates -
Answer✔️✔️-Streptococcus pyogenes
,An alpha-hemolytic, catalase negative, Gram-positive coccus from a wound culture
gave these results:
Bacitracin resistant
Bile esculin hydrolysis positive
CAMP negative
Na hippurate hydrolysis negative
PYR positive
Which of the following would be the MOST probable identification? - Answer✔️✔️-
Enterococcus species
Pseudomonas aeruginosa is often used as a bacterial species for the quality control of
Kligler iron agar. What is the expected reaction? - Answer✔️✔️-Alkaline slant/alkaline
deep
The bacterial species, a typical colony of which is illustrated in this photograph, and a
common bacterial cause of otitis externa, also known as "swimmer's ear", can be
identified as: - Answer✔️✔️-Pseudomonas aeruginosa
Based on the results of in-vitro susceptibility testing, patients with septicemia from each
of the bacterial species listed below can potentially be treated with vancomycin except: -
Answer✔️✔️-Leuconostoc species
From an isolate of Escherichia coli, Klebsiella, or Proteus species testing positive for
ESBL (Extended Spectrum Beta Lactamases), all of the following antibiotics should be
reported as resistant except: - Answer✔️✔️-Cefoxitin
Routine culture methods in microbiology rely on the principles of bacterial cultivation
and identification. Molecular testing, however relies on the detection and manipulation
, of nucleic acids, allowing the microbial genes to be detected instead of the analysis of
microbial products (for e.g. enzymes, toxins, or identifiable characteristics growth of an
organism). One disadvantage to doing molecular testing is? - Answer✔️✔️-Need for
dedicated space and equipment
Any media that supports the growth of Enterococcus species can be used to recover
Vancomycin-Resistant Enterococci. However, to improve the recovery of VRE, selective
media for VRE will provide the best sensitivity. Which of the following media is most
helpful in recovering VRE from surveillance cultures? - Answer✔️✔️-CHROM VRE agar
A day-care outbreak of diarrhea revealed an oxidase-negative, non-lactose fermenting,
nonmotile, Gram-negative rod. These characteristics presumptively indicate which
organism? - Answer✔️✔️-Shigella species
Shigella serotyping is performed by using a polyvalent somatic (O) antisera. A Shigella
species grew from culture and serotyped as Group A. Which of the following Shigella
species serotypes as Group A? - Answer✔️✔️-Shigella dysenteriae
In order to speciate a swarming Proteus organism found on an eosin methylene blue
(EMB) agar plate, the best approach would be to: - Answer✔️✔️-Determine indole
production and ornithine decarboxylase
All of the following statements are associated with MALDI-TOF mass spectrophotometry
used in clinical microbiology, except? - Answer✔️✔️-Bacteria is amplified using a laser
A patient presents with cellulitis and culture growth small beta hemolytic colonies on 5%
sheep blood agar. The gram stain of the organism is included. What is the most likely
cause of infection? - Answer✔️✔️-Streptococcus pyogenes
2024/2025 ACADEMIC YEAR
©SOPHIEBENNETT 9/1/24 2024/2025
,Micro Certification Exam Practice
Question with 100% Correct Answers
Which bacterial species is most likely represented by the spreading, wrinkled, non-
hemolytic colony appearance? - Answer✔️✔️-Burkholderia pseudomallei
Which one of the following tests should be used to monitor a patient's response to
treatment for syphilis? - Answer✔️✔️-RPR titer
A CSF specimen was sent to the laboratory for analysis. A glucose, protein, and cell
count were performed. Based on the following results, what would be the probable
cause?
Glucose -- 50 mg/dL
Protein -- 25 mg/dL
Leukocytes -- 2 cells/mm - Answer✔️✔️-Normal CSF
When performing quantitative cultures from bronchoalveolar lavage specimens, which
of the following colony forming units per millimeter indicates a true bacterial
pneumonia? - Answer✔️✔️->10 ^4 colony forming units/mL
The bacterium in the image to the right is also referred to in the quote below, what is the
identification on this bacteria?
"If a pregnant woman be attacked by erysipelas of the womb, it is fatal." - Hippocrates -
Answer✔️✔️-Streptococcus pyogenes
,An alpha-hemolytic, catalase negative, Gram-positive coccus from a wound culture
gave these results:
Bacitracin resistant
Bile esculin hydrolysis positive
CAMP negative
Na hippurate hydrolysis negative
PYR positive
Which of the following would be the MOST probable identification? - Answer✔️✔️-
Enterococcus species
Pseudomonas aeruginosa is often used as a bacterial species for the quality control of
Kligler iron agar. What is the expected reaction? - Answer✔️✔️-Alkaline slant/alkaline
deep
The bacterial species, a typical colony of which is illustrated in this photograph, and a
common bacterial cause of otitis externa, also known as "swimmer's ear", can be
identified as: - Answer✔️✔️-Pseudomonas aeruginosa
Based on the results of in-vitro susceptibility testing, patients with septicemia from each
of the bacterial species listed below can potentially be treated with vancomycin except: -
Answer✔️✔️-Leuconostoc species
From an isolate of Escherichia coli, Klebsiella, or Proteus species testing positive for
ESBL (Extended Spectrum Beta Lactamases), all of the following antibiotics should be
reported as resistant except: - Answer✔️✔️-Cefoxitin
Routine culture methods in microbiology rely on the principles of bacterial cultivation
and identification. Molecular testing, however relies on the detection and manipulation
, of nucleic acids, allowing the microbial genes to be detected instead of the analysis of
microbial products (for e.g. enzymes, toxins, or identifiable characteristics growth of an
organism). One disadvantage to doing molecular testing is? - Answer✔️✔️-Need for
dedicated space and equipment
Any media that supports the growth of Enterococcus species can be used to recover
Vancomycin-Resistant Enterococci. However, to improve the recovery of VRE, selective
media for VRE will provide the best sensitivity. Which of the following media is most
helpful in recovering VRE from surveillance cultures? - Answer✔️✔️-CHROM VRE agar
A day-care outbreak of diarrhea revealed an oxidase-negative, non-lactose fermenting,
nonmotile, Gram-negative rod. These characteristics presumptively indicate which
organism? - Answer✔️✔️-Shigella species
Shigella serotyping is performed by using a polyvalent somatic (O) antisera. A Shigella
species grew from culture and serotyped as Group A. Which of the following Shigella
species serotypes as Group A? - Answer✔️✔️-Shigella dysenteriae
In order to speciate a swarming Proteus organism found on an eosin methylene blue
(EMB) agar plate, the best approach would be to: - Answer✔️✔️-Determine indole
production and ornithine decarboxylase
All of the following statements are associated with MALDI-TOF mass spectrophotometry
used in clinical microbiology, except? - Answer✔️✔️-Bacteria is amplified using a laser
A patient presents with cellulitis and culture growth small beta hemolytic colonies on 5%
sheep blood agar. The gram stain of the organism is included. What is the most likely
cause of infection? - Answer✔️✔️-Streptococcus pyogenes
