Hematology (Clinical Laboratory)
- maintain normal blood flow
- tether(decelerating) platelets during times of injury
- enable WBCs to escape from the vessel to the surrounding tissue when needed -
ANSWER Functions of Endothelial cells
(with the 100x oil immersion objective at 1000x magnification) - ANSWER Platelet count
magnification
(with the 40x or 50x objective at 400x or 500x magnification) - ANSWER WBC count
magnification
▪ forward-anglefluorescentlightscatter
▪ right-anglefluorescentlightscatter - ANSWER Effects of Flow cytometers
0.5% to 2.5% ; blue-gray - ANSWER In the Wright-stained blood film, _____ of RBCs
exceed the average diameter and stain slightly ____
1000x magnification - ANSWER Examine the morphology of WBCs, RBCs, and platelets by
light microscopy for abnormalities of shape, diameter, color, or inclusions using what magnification
1958 - ANSWER Year when automated particle counters became available in the clinical
laboratory
1990 - ANSWER Year when automated reticulocyte counting by the TOA Corporation
(presently Sysmex) was made
5 liters - ANSWER Average volume of human blood
500x to 1000x light microscopy examination
fixed to a glass microscope slide
stained with Wright or Wright-Giemsa stain - ANSWER Analyzing RBC, WBC, and platelet
appearance:
7 to 8 μm - ANSWER RBC diameter
Accession - ANSWER registration of the specimen in the work list
acute myeloid leukemia or acute lymphoblastic leukemia - ANSWER Acute leukemia
Age-group:
- chronic lymphocytic leukemia - older than 65 years
- acute lymphoblastic leukemia - childhood leukemia - ANSWER Age group of leukemia
Anemia - ANSWER loss of oxygen-carrying capacity, often reflected in a reduced RBC count
or decreased RBC hemoglobin concentration.
anemia or polycythemia - ANSWER Before 1900, medical professionals counted RBCs in
measured volumes to detect ___ & ___
, Hematology (Clinical Laboratory)
Anisocytosis - ANSWER extreme RBC volume variability visible on the Wright-stained blood
film as variation in
diameter
Anton van Leeuwenhoek - ANSWER gave an account of RBCs
Appropriate ; clot & hemolysis ; short draws - ANSWER Must have _____ anticoagulant and
tube; free of ____ and _____ (red-tinted plasma indicating RBC damage) "_____" result in incorrect
anticoagulant-to-blood ratios
Athanasius Kircher - ANSWER Described "worms" in the blood
Bands (band neutrophils, BANDs) - ANSWER slightly less mature neutrophils with a
nonsegmented nucleus in a U or S shape
Basophilia - ANSWER elevated basophil count rare and often signals a hematologic disease
Basophils (BASOs) - ANSWER cells with dark purple, irregular cytoplasmic granules that
obscure the nucleus
Biopsy specimen, enhanced by hematoxylin and eosin (H&E) staining - ANSWER may reveal
abnormalities in bone marrow architecture indicating leukemia, bone marrow failure, or one of a
host of additional hematologic disorders.
Bone Marrow - ANSWER RBC production site
Bone marrow aspirates and biopsy specimens - ANSWER are collected and stained to
analyze nucleated cells that are the immature precursors to blood cells.
bone marrow examinations
flow cytometry immunophenotyping
cytogenetic analysis
molecular diagnosis assays - ANSWER ADVANCED HEMATOLOGY PROCEDURES
Buffy Coat - ANSWER a light-colored layer between the RBCs and plasma; contains WBCs
and platelets excluded from the hematocrit determination
cell color, size and shape
cytoplasmic inclusions
nuclear condensation - ANSWER Cell Morphology
Cellavision DM96 - ANSWER automate the WBC, RBC and platelet morphology assessment
and WBC differential processes
cerebrospinal fluid
synovial (joint) fluid
pericardial fluid
- maintain normal blood flow
- tether(decelerating) platelets during times of injury
- enable WBCs to escape from the vessel to the surrounding tissue when needed -
ANSWER Functions of Endothelial cells
(with the 100x oil immersion objective at 1000x magnification) - ANSWER Platelet count
magnification
(with the 40x or 50x objective at 400x or 500x magnification) - ANSWER WBC count
magnification
▪ forward-anglefluorescentlightscatter
▪ right-anglefluorescentlightscatter - ANSWER Effects of Flow cytometers
0.5% to 2.5% ; blue-gray - ANSWER In the Wright-stained blood film, _____ of RBCs
exceed the average diameter and stain slightly ____
1000x magnification - ANSWER Examine the morphology of WBCs, RBCs, and platelets by
light microscopy for abnormalities of shape, diameter, color, or inclusions using what magnification
1958 - ANSWER Year when automated particle counters became available in the clinical
laboratory
1990 - ANSWER Year when automated reticulocyte counting by the TOA Corporation
(presently Sysmex) was made
5 liters - ANSWER Average volume of human blood
500x to 1000x light microscopy examination
fixed to a glass microscope slide
stained with Wright or Wright-Giemsa stain - ANSWER Analyzing RBC, WBC, and platelet
appearance:
7 to 8 μm - ANSWER RBC diameter
Accession - ANSWER registration of the specimen in the work list
acute myeloid leukemia or acute lymphoblastic leukemia - ANSWER Acute leukemia
Age-group:
- chronic lymphocytic leukemia - older than 65 years
- acute lymphoblastic leukemia - childhood leukemia - ANSWER Age group of leukemia
Anemia - ANSWER loss of oxygen-carrying capacity, often reflected in a reduced RBC count
or decreased RBC hemoglobin concentration.
anemia or polycythemia - ANSWER Before 1900, medical professionals counted RBCs in
measured volumes to detect ___ & ___
, Hematology (Clinical Laboratory)
Anisocytosis - ANSWER extreme RBC volume variability visible on the Wright-stained blood
film as variation in
diameter
Anton van Leeuwenhoek - ANSWER gave an account of RBCs
Appropriate ; clot & hemolysis ; short draws - ANSWER Must have _____ anticoagulant and
tube; free of ____ and _____ (red-tinted plasma indicating RBC damage) "_____" result in incorrect
anticoagulant-to-blood ratios
Athanasius Kircher - ANSWER Described "worms" in the blood
Bands (band neutrophils, BANDs) - ANSWER slightly less mature neutrophils with a
nonsegmented nucleus in a U or S shape
Basophilia - ANSWER elevated basophil count rare and often signals a hematologic disease
Basophils (BASOs) - ANSWER cells with dark purple, irregular cytoplasmic granules that
obscure the nucleus
Biopsy specimen, enhanced by hematoxylin and eosin (H&E) staining - ANSWER may reveal
abnormalities in bone marrow architecture indicating leukemia, bone marrow failure, or one of a
host of additional hematologic disorders.
Bone Marrow - ANSWER RBC production site
Bone marrow aspirates and biopsy specimens - ANSWER are collected and stained to
analyze nucleated cells that are the immature precursors to blood cells.
bone marrow examinations
flow cytometry immunophenotyping
cytogenetic analysis
molecular diagnosis assays - ANSWER ADVANCED HEMATOLOGY PROCEDURES
Buffy Coat - ANSWER a light-colored layer between the RBCs and plasma; contains WBCs
and platelets excluded from the hematocrit determination
cell color, size and shape
cytoplasmic inclusions
nuclear condensation - ANSWER Cell Morphology
Cellavision DM96 - ANSWER automate the WBC, RBC and platelet morphology assessment
and WBC differential processes
cerebrospinal fluid
synovial (joint) fluid
pericardial fluid
