Overview of Clinical Laboratory
Hematology
- Platelet count
- Mean platelet volume
- Prothrombin time
- APTT
- Thrombin time
- Fibrinogen assay
- D-dimer assay
- Mixing studies - ANSWER types of coagulation test
.5% to 2.5%; 1.5% to 6% - ANSWER reticulocyte percent
__________ in adults
__________ in neonates
> RBC diameter
> Color of hemoglobin
> RBC shape
> Cytoplasmic inclusions - ANSWER rbc morphology
0.5% to 2% - ANSWER In the wright-stained blood film __________ of RBCs exceeds the 6
to 8 um average diameter
1:200; .85%; Isotonic
1:20; dilute acid solution - ANSWER RBC - _______ dilution; _______ NSS in _________
solution
WBC - _______ dilution; in ________ solution
7.35-7.45 - ANSWER Blood pH range is?
Acute lymphoblastic leukemia - ANSWER Most common form of childhood leukemia
anemia - ANSWER > Means loss if oxygen or oxygen carrying capacity
> Low RBC count
> Low RBC HGB
Anisocytosis - ANSWER extreme RBC volume variability on the Wright-stained blood film as
variation in diameter.
, Overview of Clinical Laboratory
Hematology
anisocytosis - ANSWER variation in size
Anton van Leeuwenhoek - ANSWER gave an account of RBCs.
Athanasius Kircher - ANSWER described "worms" in the blood
Automation or visually - ANSWER > present-day hema lab, RBC, WBC, and platelet
appearance is analyzed through this
> using 500x to 1000x light microscopy examination of cells fixed to a glass microscope slide and
stained with Wright or Wright-Giemsa stain.
Band Cell / Stab Cell - ANSWER > less differentiated or less mature neutrophil
> Pink or lavender staining granules filled with bactericidal secretions
Basophilia - ANSWER > Elevated basophil count
> is rare and often signals a hematologic disease.
Basophils - ANSWER > Are cells with dark purple, irregular cytoplasmic granules that
obscure the nucleus
> its granules contain histamine and various other proteins
blood - ANSWER Red fluid that circulates throughout the body. It is composed of pale-
yellow plasma and three types of cellular elements
blood film examination - ANSWER > It is a specialized, demanding, and fundamental CBC
activity.
> Prepare a "wedge-prep" blood stained using Wright or Wright-Giemsa stain
buffy coat - ANSWER light layer between RBC and plasma; contain WBCs and platelets
Chronic lymphocytic leukemia - ANSWER More prevalent in people older than 65 years
coagulation - ANSWER > It involves the platelets.
> Platelets are a key component of hemostasis, as previously described; plasma coagulation is the
second component.
> In a surgical operation, this test are done to determine has the ability to promote clotting.
> This system employs a complex sequence of plasma proteins, some enzymes, and some enzyme
cofactors to produce clot formation after blood vessel injury
complete blood count - ANSWER Performed on automated profiling instrument and
includes rbc, wbc, and platelet measurements
Cyanmethemoglobin or hemiglobincyanide - ANSWER in using drabkin reagent,
hemoglobin is converted to stable _______________________________ and the absorbance of the
solution is measured in a spectrophotometer at 540 nm wavelength
Hematology
- Platelet count
- Mean platelet volume
- Prothrombin time
- APTT
- Thrombin time
- Fibrinogen assay
- D-dimer assay
- Mixing studies - ANSWER types of coagulation test
.5% to 2.5%; 1.5% to 6% - ANSWER reticulocyte percent
__________ in adults
__________ in neonates
> RBC diameter
> Color of hemoglobin
> RBC shape
> Cytoplasmic inclusions - ANSWER rbc morphology
0.5% to 2% - ANSWER In the wright-stained blood film __________ of RBCs exceeds the 6
to 8 um average diameter
1:200; .85%; Isotonic
1:20; dilute acid solution - ANSWER RBC - _______ dilution; _______ NSS in _________
solution
WBC - _______ dilution; in ________ solution
7.35-7.45 - ANSWER Blood pH range is?
Acute lymphoblastic leukemia - ANSWER Most common form of childhood leukemia
anemia - ANSWER > Means loss if oxygen or oxygen carrying capacity
> Low RBC count
> Low RBC HGB
Anisocytosis - ANSWER extreme RBC volume variability on the Wright-stained blood film as
variation in diameter.
, Overview of Clinical Laboratory
Hematology
anisocytosis - ANSWER variation in size
Anton van Leeuwenhoek - ANSWER gave an account of RBCs.
Athanasius Kircher - ANSWER described "worms" in the blood
Automation or visually - ANSWER > present-day hema lab, RBC, WBC, and platelet
appearance is analyzed through this
> using 500x to 1000x light microscopy examination of cells fixed to a glass microscope slide and
stained with Wright or Wright-Giemsa stain.
Band Cell / Stab Cell - ANSWER > less differentiated or less mature neutrophil
> Pink or lavender staining granules filled with bactericidal secretions
Basophilia - ANSWER > Elevated basophil count
> is rare and often signals a hematologic disease.
Basophils - ANSWER > Are cells with dark purple, irregular cytoplasmic granules that
obscure the nucleus
> its granules contain histamine and various other proteins
blood - ANSWER Red fluid that circulates throughout the body. It is composed of pale-
yellow plasma and three types of cellular elements
blood film examination - ANSWER > It is a specialized, demanding, and fundamental CBC
activity.
> Prepare a "wedge-prep" blood stained using Wright or Wright-Giemsa stain
buffy coat - ANSWER light layer between RBC and plasma; contain WBCs and platelets
Chronic lymphocytic leukemia - ANSWER More prevalent in people older than 65 years
coagulation - ANSWER > It involves the platelets.
> Platelets are a key component of hemostasis, as previously described; plasma coagulation is the
second component.
> In a surgical operation, this test are done to determine has the ability to promote clotting.
> This system employs a complex sequence of plasma proteins, some enzymes, and some enzyme
cofactors to produce clot formation after blood vessel injury
complete blood count - ANSWER Performed on automated profiling instrument and
includes rbc, wbc, and platelet measurements
Cyanmethemoglobin or hemiglobincyanide - ANSWER in using drabkin reagent,
hemoglobin is converted to stable _______________________________ and the absorbance of the
solution is measured in a spectrophotometer at 540 nm wavelength
