MCB4203 Exam 2 Study Set @
2024-2025
Attachment to host cells - Answer colonization
Nutrient acquisition - Answer growth (Fe)
Evasion of host immune system - Answer allows persistence and survival
Dissemination - Answer species survival inside host, shed from host and survive
Species survival outside host - Answer "pre‐infection" in environment like soil lie and
wait to colonize host cells
Disease symptoms - Answer result of microbe and/or its products + host
response (can dictate severity), undesirable disease process
Virulence factors - Answer molecules produced or strategies used by disease
causing bacteria that enable them to infect and survive in the host
Growth Phase - Answer Virulence factor is growth phase dependent
*Exponential Phase
->Colonization
- Mga (adhesion, internalization, immune evasion)
*Early Stationary
->Persistence Phase
- Nra/RofA, RALPS (MSCRAMMS, Intracellular
persistence, host-cell interaction)
*Stationary Phase
->Spread
- Rgg/RopB (secreted proteins, proteolysis)
Other Bacteria
- endospore formation
- Autolysis
, - competence development
Pre-Infection - Answer -Survival in external environment
(nutrient availability, adherence sites, exposure to chemicals/predators, environ.
extremes i.e. pH, desiccation, UV, Temp.)
-Strategies
1. endospore formation
2. Metabolic dormancy
3. Resistance to DNA damage/protein oxidation
4. Attachment/ growth on abiotic objects
5. Intermediate Hosts (insect/amoeba)
Biofilms - Answer -multicellular community of bacteria encompassed in a protective
matrix, used to adhere attach proved protective film, (single or mixed species)
-Display differences in: gene expression,physiology, stress tolerance (liquid cultures),
increased resistance to antibiotic and immune system!
-Infections due to biofilm growth (Chronic Infections: dental plaque, endocarditis,
surgical implants/indwelling medical device infection, Cystic Fibrosis CF)
Motility - Answer chemotaxis- travel bw environments
Flagella
-long helical structure
-polymerized subunits of flagellin protein
-polar (one end) vs. lateral (sides)
-flagellin proteins are relatively conserved (TLR-5) and antigenic (H Ag of motile
bacteria)
-expression is usually turned off in host (regulated by phase variation "on-off switch)
Movement proton motive force drives energy to move bacteria
Colonization of Host Surfaces - Answer 1. penetration of the skin and mucin layer
2. slgA, AMPS, iron acquisition
3. Adherence to host cell/tissues
Penetration of the Skin - Answer no microbe known that can penetrate skin without
2024-2025
Attachment to host cells - Answer colonization
Nutrient acquisition - Answer growth (Fe)
Evasion of host immune system - Answer allows persistence and survival
Dissemination - Answer species survival inside host, shed from host and survive
Species survival outside host - Answer "pre‐infection" in environment like soil lie and
wait to colonize host cells
Disease symptoms - Answer result of microbe and/or its products + host
response (can dictate severity), undesirable disease process
Virulence factors - Answer molecules produced or strategies used by disease
causing bacteria that enable them to infect and survive in the host
Growth Phase - Answer Virulence factor is growth phase dependent
*Exponential Phase
->Colonization
- Mga (adhesion, internalization, immune evasion)
*Early Stationary
->Persistence Phase
- Nra/RofA, RALPS (MSCRAMMS, Intracellular
persistence, host-cell interaction)
*Stationary Phase
->Spread
- Rgg/RopB (secreted proteins, proteolysis)
Other Bacteria
- endospore formation
- Autolysis
, - competence development
Pre-Infection - Answer -Survival in external environment
(nutrient availability, adherence sites, exposure to chemicals/predators, environ.
extremes i.e. pH, desiccation, UV, Temp.)
-Strategies
1. endospore formation
2. Metabolic dormancy
3. Resistance to DNA damage/protein oxidation
4. Attachment/ growth on abiotic objects
5. Intermediate Hosts (insect/amoeba)
Biofilms - Answer -multicellular community of bacteria encompassed in a protective
matrix, used to adhere attach proved protective film, (single or mixed species)
-Display differences in: gene expression,physiology, stress tolerance (liquid cultures),
increased resistance to antibiotic and immune system!
-Infections due to biofilm growth (Chronic Infections: dental plaque, endocarditis,
surgical implants/indwelling medical device infection, Cystic Fibrosis CF)
Motility - Answer chemotaxis- travel bw environments
Flagella
-long helical structure
-polymerized subunits of flagellin protein
-polar (one end) vs. lateral (sides)
-flagellin proteins are relatively conserved (TLR-5) and antigenic (H Ag of motile
bacteria)
-expression is usually turned off in host (regulated by phase variation "on-off switch)
Movement proton motive force drives energy to move bacteria
Colonization of Host Surfaces - Answer 1. penetration of the skin and mucin layer
2. slgA, AMPS, iron acquisition
3. Adherence to host cell/tissues
Penetration of the Skin - Answer no microbe known that can penetrate skin without
